Acquisition and maintenance of pluripotency are influenced by fibroblast growth factor, leukemia inhibitory factor, and 2i in bovine-induced pluripotent stem cells

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversity of Guelph-
Autor(es): dc.contributorDavis-
Autor(es): dc.creatorBotigelli, Ramon Cesar-
Autor(es): dc.creatorPieri, Naira Carolina Godoy-
Autor(es): dc.creatorBessi, Brendon William-
Autor(es): dc.creatorMachado, Lucas Simões-
Autor(es): dc.creatorBridi, Alessandra-
Autor(es): dc.creatorde Souza, Aline Fernanda-
Autor(es): dc.creatorRecchia, Kaiana-
Autor(es): dc.creatorNeto, Paulo Fantinato-
Autor(es): dc.creatorRoss, Pablo Juan-
Autor(es): dc.creatorBressan, Fabiana Fernandes-
Autor(es): dc.creatorNogueira, Marcelo Fábio Gouveia-
Data de aceite: dc.date.accessioned2025-08-21T21:56:17Z-
Data de disponibilização: dc.date.available2025-08-21T21:56:17Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2022-09-14-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3389/fcell.2022.938709-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/247683-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/247683-
Descrição: dc.descriptionSeveral opportunities for embryo development, stem cell maintenance, cell fate, and differentiation have emerged using induced pluripotent stem cells (iPSCs). However, the difficulty in comparing bovine iPSCs (biPSCs) with embryonic stem cells (ESCs) was a challenge for many years. Here, we reprogrammed fetal fibroblasts by transient expression of the four transcription factors (Oct4, Sox2, Klf4, and c-Myc, collectively termed “OSKM” factors) and cultured in iPSC medium, supplemented with bFGF, bFGF2i, leukemia inhibitory factor (LIF), or LIF2i, and then compared these biPSC lines with bESC to evaluate the pluripotent state. biPSC lines were generated in all experimental groups. Particularly, reprogrammed cells treated with bFGF were more efficient in promoting the acquisition of pluripotency. However, LIF2i treatment did not promote continuous self-renewal. biPSCs (line 2) labeled with GFP were injected into early embryos (day 4.5) to assess the potential to contribute to chimeric blastocysts. The biPSC lines show a pluripotency state and are differentiated into three embryonic layers. Moreover, biPSCs and bESCs labeled with GFP were able to contribute to chimeric blastocysts. Additionally, biPSCs have shown promising potential for contributing to chimeric blastocysts and for future studies.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionMultiuser Facility (FitoFarmaTec) Department of Pharmacology Biosciences Institute (IBB) São Paulo State University (UNESP)-
Descrição: dc.descriptionLaboratory of Molecular Morphophysiology and Development (LMMD) Department of Veterinary Medicine Faculty of Animal Science and Food Engineering (FZEA) University of São Paulo (USP)-
Descrição: dc.descriptionPaulista School of Medicine (EPM) Laboratory of Neurobiology Department of Biochemistry Federal University of São Paulo (UNIFESP)-
Descrição: dc.descriptionLaboratory Biomedical Science Department of Biomedical Science Ontario Veterinary College (OVC) University of Guelph-
Descrição: dc.descriptionLaboratory De partment of Animal Science University of California Davis-
Descrição: dc.descriptionSchool of Sciences and Languages Laboratory of Embryonic Micromanipulation Department of Biological Sciences São Paulo State University (UNESP)-
Descrição: dc.descriptionMultiuser Facility (FitoFarmaTec) Department of Pharmacology Biosciences Institute (IBB) São Paulo State University (UNESP)-
Descrição: dc.descriptionSchool of Sciences and Languages Laboratory of Embryonic Micromanipulation Department of Biological Sciences São Paulo State University (UNESP)-
Descrição: dc.descriptionFAPESP: 2012/50533-2 2015/26818-5 2016/16841-2 2013/08135-2 2019/10751-0 23038.006964/2014-43 433133/2018-0-
Idioma: dc.languageen-
Relação: dc.relationFrontiers in Cell and Developmental Biology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectbovine-
Palavras-chave: dc.subjectcellular reprogramming-
Palavras-chave: dc.subjectembryonic stem cells-
Palavras-chave: dc.subjectinduced pluripotent stem cells-
Palavras-chave: dc.subjectpluripotency maintenance-
Título: dc.titleAcquisition and maintenance of pluripotency are influenced by fibroblast growth factor, leukemia inhibitory factor, and 2i in bovine-induced pluripotent stem cells-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.