Antagonism of cysteinyl leukotriene receptors by zafirlukast modulated acute inflammatory reaction in tilapia, Oreochromis niloticus

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorFederal University of Rondônia (UNIR)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorBrazil University – (UB)-
Autor(es): dc.creatorAracati, Mayumi Fernanda-
Autor(es): dc.creatorLuporini de Oliveira, Susana-
Autor(es): dc.creatorRodrigues, Leticia Franchin-
Autor(es): dc.creatorCarlino da Costa, Camila-
Autor(es): dc.creatorCristina de Moraes, Alessandra-
Autor(es): dc.creatorda Rosa Prado, Ed Johnny-
Autor(es): dc.creatorFernandes, Dayanne Carla-
Autor(es): dc.creatorVaz Farias, Thais Heloisa-
Autor(es): dc.creatorEto, Silas Fernandes-
Autor(es): dc.creatorCharlie-Silva, Ives-
Autor(es): dc.creatorde Andrade Belo, Marco Antonio-
Data de aceite: dc.date.accessioned2025-08-21T19:54:05Z-
Data de disponibilização: dc.date.available2025-08-21T19:54:05Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2022-10-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.fsi.2022.09.030-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/247654-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/247654-
Descrição: dc.descriptionTo identify activation pathways and effector mechanisms of innate immunity in fish has become relevant for the sanitary management of intensive fish farming. However, little is known about the blocking of cysteinyl leukotrienes receptors (CysLTRs) and their effects in teleost fish. Our study evaluated the anti-inflammatory effect of 250 and 500 μg zafirlukast (antagonist of CysLTRs)/kg b.w., administered orally in the diet, during acute inflammatory reaction induced by Aeromonas hydrophila bacterins in Oreochromis niloticus. 80 tilapia were distributed in 10 aquariums (100L of water each, n = 8) to constitute three treatments: Control (inoculated with A. hydrophila bacterin and untreated); Treated with 250 μg or 500 μg of zafirlukast/kg b.w. and inoculated. To be evaluated in three periods: 6, 24 and 48 h post-inoculation (HPI), totaling nine aquariums. A tenth group was sampled without any stimulus to constitute reference values (Physiological standards). Tilapia treated with zafirlukast demonstrated dose-response effect in the decrease of accumulated inflammatory cells, strongly influenced by granulocytes and macrophages. Zafirlukast treated-tilapia showed decrease in blood leukocyte counts (mainly neutrophils, and monocytes) and reactive oxygen species production. Treatment with zafirlukast resulted in down-regulation of ceruloplasmin, complement 3, alpha2-macroglobulin, transferrin and apolipoprotein A1, as well as up-regulation of haptoglobin. Our study provided convincing results in the pathophysiology of tilapia inflammatory reaction, considering that treatment with zafirlukast, antagonist of cysteinyl leukotriene receptors, resulted in a dose-response effect by suppressing the dynamics between leukocytes in the bloodstream and cell accumulation in the inflamed focus, as well as modulated the leukocyte oxidative burst and the acute phase protein response.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Preventive Veterinary Medicine São Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Veterinary Medicine Federal University of Rondônia (UNIR), RO-
Descrição: dc.descriptionAquaculture Center São Paulo State University (CAUNESP), SP-
Descrição: dc.descriptionDeparment of Pharmacology University of São Paulo-ICB/USP, SP-
Descrição: dc.descriptionLaboratory of Animal Pharmacology and Toxicology Brazil University – (UB), SP-
Descrição: dc.descriptionDepartment of Preventive Veterinary Medicine São Paulo State University (UNESP), SP-
Descrição: dc.descriptionAquaculture Center São Paulo State University (CAUNESP), SP-
Descrição: dc.descriptionFAPESP: 2017/03094-7-
Formato: dc.format323-331-
Idioma: dc.languageen-
Relação: dc.relationFish and Shellfish Immunology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCichlids-
Palavras-chave: dc.subjectCysteine leukotrienes-
Palavras-chave: dc.subjectInnate immunity-
Palavras-chave: dc.subjectLipoxygenase-
Palavras-chave: dc.subjectMacrophages-
Palavras-chave: dc.subjectNeutrophils-
Título: dc.titleAntagonism of cysteinyl leukotriene receptors by zafirlukast modulated acute inflammatory reaction in tilapia, Oreochromis niloticus-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.