Detection of Prostate Cancer Biomarker PCA3 by Using Aptasensors

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorCrulhas, Bruno P.-
Autor(es): dc.creatorBasso, Caroline R.-
Autor(es): dc.creatorCastro, Gustavo R.-
Autor(es): dc.creatorPedrosa, Valber A.-
Data de aceite: dc.date.accessioned2025-08-21T16:27:16Z-
Data de disponibilização: dc.date.available2025-08-21T16:27:16Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2022-10-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.2174/0929867329666220607162250-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/247599-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/247599-
Descrição: dc.descriptionBackground: Prostate cancer cells have very high PCA3 messenger RNA lev-els, which turns them into one of the new biomarkers for prostate cancer prognosis and di-agnosis. Objective: Our goal here is to develop a new aptasensor to detect PCA3 release by the cancer cell. Methods: DNA hairpin containing PCA3 aptamer was thiolated, conjugated to methy-lene blue (MB) redox probe, and immobilized on gold electrode through self-assembly to detect label-free cancer cells. Results: Our data have evidenced stable and sensitive sensors presenting a wide linear detection range (0-150ng/mL). In addition, monitoring PCA3 released by different types of prostate cells can provide in-depth knowledge about prostate cancer dynamics; therefore, it is a powerful platform for earlier clinical diagnostic. The released PCA3 can vary de-pending on the type of adopted prostate cells. Conclusion: PCA3 release was monitored in a group of cells for 2 h; it showed signifi-cantly higher expression in both LNCaP and PC-3 cells. This strategy provides a unique and simple methodology to achieve more sensitive and specific PCA3 detection; thus, it emerged as a promising tool for early cost-effective diagnosis.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Chemistry and Biochemistry Institute of Bioscience UNESP, SP-
Descrição: dc.descriptionDepartment of Chemistry and Biochemistry Institute of Bioscience UNESP, SP-
Descrição: dc.descriptionFAPESP: 2019/13411-5-
Formato: dc.format5895-5902-
Idioma: dc.languageen-
Relação: dc.relationCurrent Medicinal Chemistry-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectaptamer-
Palavras-chave: dc.subjectAptasensor-
Palavras-chave: dc.subjectbiomarker-
Palavras-chave: dc.subjectcancer cell-
Palavras-chave: dc.subjectPCA3-
Palavras-chave: dc.subjectreleasing protein-
Título: dc.titleDetection of Prostate Cancer Biomarker PCA3 by Using Aptasensors-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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