High sperm concentration during cryopreservation decreases post-thaw motility percentage without compromising in vitro fertilization outcomes in common carp

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorResearch Institute of Fish Culture and Hydrobiology-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorSotnikov, Anatolii-
Autor(es): dc.creatorRodina, Marek-
Autor(es): dc.creatorStechkina, Taisiya-
Autor(es): dc.creatorBenevente, Cristiane Fernanda-
Autor(es): dc.creatorGela, David-
Autor(es): dc.creatorBoryshpolets, Sergii-
Autor(es): dc.creatorKholodnyy, Vitaliy-
Autor(es): dc.creatorLinhart, Otomar-
Autor(es): dc.creatorDzyuba, Borys-
Data de aceite: dc.date.accessioned2025-08-21T18:15:40Z-
Data de disponibilização: dc.date.available2025-08-21T18:15:40Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-01-14-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.aquaculture.2022.738746-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/247598-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/247598-
Descrição: dc.descriptionTo avoid mechanical compression of spermatozoa during the cryopreservation, a high dilution ratio with the cryoprotective medium is applied. Due to this, the sample volume increases, which entails increased cryobank capacity. Moreover, the high volume of cryomedias does not allow to fertilize of large volumes of eggs in practical artificial reproduction of common carp aquaculture. The current study demonstrated that reasonable lowering of dilution rates might still be effective for carp sperm cryobanking —elevation of spermatozoa concentration up to 13 × 109 spz mL−1 resulted in a significant decrease in post-thaw sperm motility percentage to 20% compared with 39% in 0.5 × 109 spz mL−1. Nevertheless, no significant differences in sperm kinetic parameters (VCL and LIN) were found in this case. The fertilization outcome (embryo development and hatching rates) was similar after applying thawed sperm samples with “optimal” (2.2–2.4 × 109 spz mL−1) and “sub-optimal” (11.0–13.0 × 109 spz mL−1) concentrations (sperm/egg ratio at fertilization was in the range of 0.3–4.5 × 105 spz/egg). Thus, applying a low dilution rate such as one part of sperm to one of the cryoprotective mediums is favorable for decreasing cryo-storage space and the sperm volume needed to fertilize big egg numbers. The experiment also shows that the 4.5 × 105 spz/egg ratio is not sufficient for good fertilization, and it is necessary to use higher sperm concentrations per egg or improve the method of fertilization.-
Descrição: dc.descriptionGrantová Agentura České Republiky-
Descrição: dc.descriptionMinisterstvo Zemědělství-
Descrição: dc.descriptionUniversity of South Bohemia in České Budějovice Faculty of Fisheries and Protection of Waters South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II-
Descrição: dc.descriptionUNESP –Paulista State University “Júlio de Mesquita Filho” Ilha Solteira Campus Department of Biology and Zootechnics LINEO - Neotropical Ichthyology Laboratory - Rua Monção, 226 - North Zone, São Paulo-
Descrição: dc.descriptionPost-graduate Program in Biological Sciences (Zoology) - R. Prof. Dr. Antônio Celso Wagner Zanin, 250 - Rubião Junior District, São Paulo-
Descrição: dc.descriptionUNESP –Paulista State University “Júlio de Mesquita Filho” Ilha Solteira Campus Department of Biology and Zootechnics LINEO - Neotropical Ichthyology Laboratory - Rua Monção, 226 - North Zone, São Paulo-
Descrição: dc.descriptionGrantová Agentura České Republiky: N 22-14069S-
Descrição: dc.descriptionMinisterstvo Zemědělství: NAZV QK21010141-
Idioma: dc.languageen-
Relação: dc.relationAquaculture-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectComputer-assisted sperm analysis-
Palavras-chave: dc.subjectCryopreservation-
Palavras-chave: dc.subjectCypriniformes-
Palavras-chave: dc.subjectSperm concentration-
Palavras-chave: dc.subjectSperm motility-
Palavras-chave: dc.subjectSperm spectrophotometry-
Título: dc.titleHigh sperm concentration during cryopreservation decreases post-thaw motility percentage without compromising in vitro fertilization outcomes in common carp-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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