MiR-150 regulates the Leishmania infantum parasitic load and granzyme B levels in peripheral blood mononuclear cells of dogs with canine visceral leishmaniosis

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorSoares, Matheus Fujimura-
Autor(es): dc.creatorCosta, Sidnei Ferro-
Autor(es): dc.creatorde Freitas, Jéssica Henrique-
Autor(es): dc.creatorRebech, Gabriela Torres-
Autor(es): dc.creatordos Santos, Marilene Oliveira-
Autor(es): dc.creatorde Lima, Valéria Marçal Felix-
Data de aceite: dc.date.accessioned2025-08-21T22:42:44Z-
Data de disponibilização: dc.date.available2025-08-21T22:42:44Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-08-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.vetpar.2023.109958-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/247472-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/247472-
Descrição: dc.descriptionLeishmania infantum causes visceral leishmaniosis, a neglected tropical disease that can modulate the host immune response by altering the expression of small non-coding RNAs called microRNAs (miRNAs). Some miRNAs are differentially expressed in peripheral blood mononuclear cells (PBMCs) of dogs with canine visceral leishmaniosis (CanL), like the down-regulated miR-150. Even though miR-150 is negatively correlated with L. infantum parasitic load, it is unclear if miR-150 directly affects L. infantum parasitic load and (if so) how this miRNA would contribute to infection. Here, we isolated PBMCs from 14 naturally infected dogs (CanL group) and six healthy dogs (Control group) and treated them in vitro with miR-150 mimic or inhibitor. We measured L. infantum parasitic load using qPCR and compared treatments. We also measured miR-150 in silico predicted target protein levels (STAT1, TNF-α, HDAC8, and GZMB) using flow cytometry or enzyme-linked immunosorbent assays. Increasing miR-150 activity diminished L. infantum parasitic load in CanL PBMCs. We also found that inhibition of miR-150 reduced GZMB (granzyme B) levels. These findings demonstrate that miR-150 plays an important role in L. infantum infection in canine PBMCs, and they merit further studies aiming at drug development.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Clinical Medicine Surgery and Animal Reproduction São Paulo State University (UNESP) School of Veterinary Medicine, 793 Clóvis Pestana St-
Descrição: dc.descriptionDepartment of Clinical Medicine Surgery and Animal Reproduction São Paulo State University (UNESP) School of Veterinary Medicine, 793 Clóvis Pestana St-
Descrição: dc.descriptionFAPESP: 2020/00565-1-
Descrição: dc.descriptionFAPESP: 2021/07283-4-
Idioma: dc.languageen-
Relação: dc.relationVeterinary Parasitology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectDog-
Palavras-chave: dc.subjectEpigenetics-
Palavras-chave: dc.subjectInflammation-
Palavras-chave: dc.subjectMicroRNA-
Palavras-chave: dc.subjectPeripheral blood mononuclear cell-
Título: dc.titleMiR-150 regulates the Leishmania infantum parasitic load and granzyme B levels in peripheral blood mononuclear cells of dogs with canine visceral leishmaniosis-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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