Adipose-derived stem cells (ASCs) culture in spinner flask: improving the parameters of culture in a microcarrier-based system

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorSchool of Medicine of Marilia-
Autor(es): dc.contributorButantan Institute-
Autor(es): dc.creatorSimão, Vinícius Augusto-
Autor(es): dc.creatorBrand, Heloisa-
Autor(es): dc.creatorda Silveira-Antunes, Roseli Nunes-
Autor(es): dc.creatorFukasawa, Josianne Thomazini-
Autor(es): dc.creatorLeme, Jaci-
Autor(es): dc.creatorTonso, Aldo-
Autor(es): dc.creatorRibeiro-Paes, João Tadeu-
Data de aceite: dc.date.accessioned2025-08-21T22:42:49Z-
Data de disponibilização: dc.date.available2025-08-21T22:42:49Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2022-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s10529-023-03367-x-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/247380-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/247380-
Descrição: dc.descriptionPrior to clinical use, extensive in vitro proliferation of human adipose-derived stem cells (ASCs) is required. Among the current options, spinner-type stirred flasks, which use microcarriers to increase the yield of adherent cells, are recommended. Here, we propose a methodology for ASCs proliferation through cell suspension culture using Cultispher-S® microcarriers (MC) under agitation in a spinner flask, with the aim of establishing a system that reconciles the efficiency of cell yield with high viability of the culture during two distinct phases: seeding and proliferation. The results showed that cell adhesion was potentiated under intermittent stirring at 70 rpm in the presence of 10% FBS for an initial cell concentration of 2.4 × 104 cells/mL in the initial 24 h of cultivation. In the proliferation phase, kinetic analysis showed that cell growth was higher under continuous agitation at 50 rpm with a culture medium renewal regime of 50% every 72 h, which was sufficient to maintain the culture at optimal levels of nutrients and metabolites for up to nine days of cultivation, representing an 11.1-fold increase and a maximum cell productivity of 422 cells/mL/h (1.0 × 105 viable cells/mL). ASCs maintained the immunophenotypic characteristics and mesodermal differentiation potential of both cell lines from different donors. The established protocol represents a more efficient and cost-effective method to obtain a high proliferation rate of ASCs in a microcarrier-based system, which is necessary for large-scale use in cell therapy, highlighting that the manipulation of critical parameters optimizes the ASCs production process.-
Descrição: dc.descriptionDepartment of Genetics School of Medicine University of São Paulo, São Paulo-
Descrição: dc.descriptionDepartment of Biotechnology School of Sciences and Letters São Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionDepartment of Hematology Hemocenter School of Medicine of Marilia, São Paulo-
Descrição: dc.descriptionCenter for Development and Innovation Laboratory of Viral Biotechnology Butantan Institute, São Paulo-
Descrição: dc.descriptionDepartment of Chemical Engineering Polytechnic School University of São Paulo, São Paulo-
Descrição: dc.descriptionDepartment of Biotechnology School of Sciences and Letters São Paulo State University (UNESP), São Paulo-
Idioma: dc.languageen-
Relação: dc.relationBiotechnology Letters-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAdipose tissue-
Palavras-chave: dc.subjectCell proliferation-
Palavras-chave: dc.subjectCultispher-S® microcarrier-
Palavras-chave: dc.subjectHuman mesenchymal stem stromal cells-
Palavras-chave: dc.subjectKinetic analysis-
Palavras-chave: dc.subjectSpinner flask-
Palavras-chave: dc.subjectSuspension culture-
Título: dc.titleAdipose-derived stem cells (ASCs) culture in spinner flask: improving the parameters of culture in a microcarrier-based system-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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