Comparative assessment of the bacterial communities associated with Anopheles darlingi immature stages and their breeding sites in the Brazilian Amazon

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Autor(es): dc.contributorUppsala University-
Autor(es): dc.contributorSwedish University of Agricultural Sciences (SLU)-
Autor(es): dc.contributorUniversidade do Estado do Amazonas-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorIndiana University-
Autor(es): dc.contributorLund University-
Autor(es): dc.contributorInstituto Nacional de Pesquisas da Amazônia-
Autor(es): dc.contributorUniversidade Federal do Amazonas-
Autor(es): dc.creatorMosquera, Katherine D.-
Autor(es): dc.creatorNilsson, Louise K. J.-
Autor(es): dc.creatorde Oliveira, Marta Rodrigues-
Autor(es): dc.creatorRocha, Elerson Matos-
Autor(es): dc.creatorMarinotti, Osvaldo-
Autor(es): dc.creatorHåkansson, Sebastian-
Autor(es): dc.creatorTadei, Wanderli P.-
Autor(es): dc.creatorde Souza, Antonia Queiroz Lima-
Autor(es): dc.creatorTerenius, Olle-
Data de aceite: dc.date.accessioned2025-08-21T18:08:48Z-
Data de disponibilização: dc.date.available2025-08-21T18:08:48Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1186/s13071-023-05749-6-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/247336-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/247336-
Descrição: dc.descriptionBackground: The neotropical anopheline mosquito Anopheles darlingi is a major malaria vector in the Americas. Studies on mosquito-associated microbiota have shown that symbiotic bacteria play a major role in host biology. Mosquitoes acquire and transmit microorganisms over their life cycle. Specifically, the microbiota of immature forms is largely acquired from their aquatic environment. Therefore, our study aimed to describe the microbial communities associated with An. darlingi immature forms and their breeding sites in the Coari municipality, Brazilian Amazon. Methods: Larvae, pupae, and breeding water were collected in two different geographical locations. Samples were submitted for DNA extraction and high-throughput 16S rRNA gene sequencing was conducted. Microbial ecology analyses were performed to explore and compare the bacterial profiles of An. darlingi and their aquatic habitats. Results: We found lower richness and diversity in An. darlingi microbiota than in water samples, which suggests that larvae are colonized by a subset of the bacterial community present in their breeding sites. Moreover, the bacterial community composition of the immature mosquitoes and their breeding water differed according to their collection sites, i.e., the microbiota associated with An. darlingi reflected that in the aquatic habitats where they developed. The three most abundant bacterial classes across the An. darlingi samples were Betaproteobacteria, Clostridia, and Gammaproteobacteria, while across the water samples they were Gammaproteobacteria, Bacilli, and Alphaproteobacteria. Conclusions: Our findings reinforce the current evidence that the environment strongly shapes the composition and diversity of mosquito microbiota. A better understanding of mosquito–microbe interactions will contribute to identifying microbial candidates impacting host fitness and disease transmission. Graphical Abstract: [Figure not available: see fulltext.].-
Descrição: dc.descriptionCentre for Interdisciplinary Mathematics, Uppsala Universitet-
Descrição: dc.descriptionCarl Tryggers Stiftelse för Vetenskaplig Forskning-
Descrição: dc.descriptionUppsala Universitet-
Descrição: dc.descriptionVetenskapsrådet-
Descrição: dc.descriptionDepartment of Cell and Molecular Biology Biomedical Centre (BMC) Uppsala University-
Descrição: dc.descriptionDepartment of Ecology Swedish University of Agricultural Sciences (SLU)-
Descrição: dc.descriptionPrograma de Pós-graduação em Biodiversidade e Biotecnologia (PPG-BIONORTE) Universidade do Estado do Amazonas-
Descrição: dc.descriptionDepartment of Entomology and Acarology Luiz de Queiroz College of Agriculture (ESALQ) University of São Paulo-
Descrição: dc.descriptionSchool of Agricultural Sciences Department of Bioprocesses and Biotechnology Central Multi User Laboratory São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Biology Indiana University-
Descrição: dc.descriptionDivision of Applied Microbiology Department of Chemistry Lund University-
Descrição: dc.descriptionLaboratório de Malária e Dengue Instituto Nacional de Pesquisas da Amazônia-
Descrição: dc.descriptionFaculdade de Ciências Agrárias Universidade Federal do Amazonas-
Descrição: dc.descriptionSchool of Agricultural Sciences Department of Bioprocesses and Biotechnology Central Multi User Laboratory São Paulo State University (UNESP)-
Descrição: dc.descriptionVetenskapsrådet: 348-2012-622-
Idioma: dc.languageen-
Relação: dc.relationParasites and Vectors-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAmazon-
Palavras-chave: dc.subjectAnopheles darlingi-
Palavras-chave: dc.subjectBreeding sites-
Palavras-chave: dc.subjectMalaria-
Palavras-chave: dc.subjectMicrobiota-
Título: dc.titleComparative assessment of the bacterial communities associated with Anopheles darlingi immature stages and their breeding sites in the Brazilian Amazon-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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