Naringenin and proanthocyanidins pre-treatment decreases synthesis and activity of gelatinases induced by zoledronic acid in a dental implant surface in vitro model

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorRibeirão Preto University (UNAERP)-
Autor(es): dc.creatorCardoso, Lais Medeiros-
Autor(es): dc.creatorPansani, Taisa Nogueira-
Autor(es): dc.creatorde Souza Costa, Carlos Alberto-
Autor(es): dc.creatorBasso, Fernanda Gonçalves-
Data de aceite: dc.date.accessioned2025-08-21T18:03:12Z-
Data de disponibilização: dc.date.available2025-08-21T18:03:12Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.archoralbio.2023.105703-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/247281-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/247281-
Descrição: dc.descriptionObjective: To assess the effects of pre-treatment with proanthocyanidins (PA) flavonoids, from grape seed extract, and synthetic naringenin (NA) on the synthesis of matrix metalloproteinases (MMPs) gelatinases and their tissue inhibitors (TIMPs), as well as the gelatinolytic activity of MMPs by human gingival fibroblasts (HGF) and osteoblasts (Ob) exposed to zoledronic acid (ZA) in a dental implant surface in vitro model. Design: The highest non-cytotoxic concentrations of NA and PA were determined for HGF (10 μg/mL; defined by previous study) and Ob (0.5 μg/mL; defined by prestoBlue assay). Then, HFG and Ob were individually seeded onto titanium discs, and after 24 h, cells were pre-treated (or not) with NA or PA, followed (or not) by exposure to ZA. Next, MMP-2, MMP-9, TIMP-1, TIMP-2 synthesis (ELISA), and gelatinolytic activity (in situ zymography) was evaluated. Data were analyzed by one-way ANOVA and Tukey tests (α = 0.05). Results: ZA treatment increased the synthesis (p < 0.05) and activity of MMPs; flavonoids pre-treatment controlled ZA-induced gelatinolytic effects, down-regulating MMPs synthesis (p < 0.05) and activity by HGF and Ob. For HGF, NA and PA pre-treatment did not up-regulate TIMP synthesis after ZA exposure (p > 0.05); for Ob, TIMP-2 was up-regulated (p < 0.05) by flavonoids, followed by ZA. Conclusions: NA and PA pre-treatment provides interesting results in the modulation of ZA deleterious effects, down-regulating MMP-2 and MMP-9 synthesis and activity by HGF and Ob and up-regulating TIMP-2 by Ob.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics São Paulo State University (UNESP) – School of Dentistry, 1680 Humaitá Street, São Paulo-
Descrição: dc.descriptionDepartment of Physiology and Pathology São Paulo State University (UNESP) – School of Dentistry, 1680 Humaitá Street, São Paulo-
Descrição: dc.descriptionDepartment of Dentistry Ribeirão Preto University (UNAERP), 2201 Costábile Romano Avenue, São Paulo-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics São Paulo State University (UNESP) – School of Dentistry, 1680 Humaitá Street, São Paulo-
Descrição: dc.descriptionDepartment of Physiology and Pathology São Paulo State University (UNESP) – School of Dentistry, 1680 Humaitá Street, São Paulo-
Descrição: dc.descriptionCNPq: 302108/2019-0-PQ2-
Descrição: dc.descriptionCNPq: 303574/2022-4-
Idioma: dc.languageen-
Relação: dc.relationArchives of Oral Biology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectFibroblasts-
Palavras-chave: dc.subjectFlavonoids-
Palavras-chave: dc.subjectMatrix metalloproteinases-
Palavras-chave: dc.subjectOsteoblasts-
Palavras-chave: dc.subjectTitanium-
Palavras-chave: dc.subjectZoledronic acid-
Título: dc.titleNaringenin and proanthocyanidins pre-treatment decreases synthesis and activity of gelatinases induced by zoledronic acid in a dental implant surface in vitro model-
Tipo de arquivo: dc.typelivro digital-
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