Laser-Modified Ti Surface Improves Paracrine Osteogenesis by Modulating the Expression of DKK1 in Osteoblasts

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade Federal de Goiás (UFG)-
Autor(es): dc.contributorFederal University of Maranhão-
Autor(es): dc.contributorUniversidade Federal de Uberlândia (UFU)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorTeixeira, Jorge Felipe Lima-
Autor(es): dc.creatorde Souza, João Antônio Chaves-
Autor(es): dc.creatorMagalhães, Fernando Augusto Cintra-
Autor(es): dc.creatorde Oliveira, Guilherme José Pimentel Lopes-
Autor(es): dc.creatorde Santis, José Bernardo-
Autor(es): dc.creatorde Souza Costa, Carlos Alberto-
Autor(es): dc.creatorde Souza, Pedro Paulo Chaves-
Data de aceite: dc.date.accessioned2025-08-21T17:05:33Z-
Data de disponibilização: dc.date.available2025-08-21T17:05:33Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-04-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/jfb14040224-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/247261-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/247261-
Descrição: dc.descriptionTitanium surface modifications are widely used to modulate cellular behavior by recognition of topographical cues. However, how those modifications affect the expression of mediators that will influence neighboring cells is still elusive. This study aimed to evaluate the effects of conditioned media from osteoblasts cultured on laser-modified titanium surfaces on the differentiation of bone marrow cells in a paracrine manner and to analyze the expression of Wnt pathway inhibitors. Mice calvarial osteoblasts were seeded on polished (P) and Yb:YAG laser-irradiated (L) Ti surfaces. Osteoblast culture media were collected and filtered on alternate days to stimulate mice BMCs. Resazurin assay was performed every other day for 20 days to check BMC viability and proliferation. After 7 and 14 days of BMCs maintained with osteoblasts P and L-conditioned media, alkaline phosphatase activity, Alizarin Red staining, and RT-qPCR were performed. ELISA of conditioned media was conducted to investigate the expression of Wnt inhibitors Dickkopf-1 (DKK1) and Sclerostin (SOST). BMCs showed increased mineralized nodule formation and alkaline phosphatase activity. The L-conditioned media enhanced the BMC mRNA expression of bone-related markers Bglap, Alpl, and Sp7. L-conditioned media decreased the expression of DKK1 compared with P-conditioned media. The contact of osteoblasts with Yb:YAG laser-modified Ti surfaces induces the regulation of the expression of mediators that affect the osteoblastic differentiation of neighboring cells. DKK1 is among these regulated mediators.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionDepartment of Physiology and Pathology School of Dentistry São Paulo State University-
Descrição: dc.descriptionSchool of Dentistry Federal University of Goiás-
Descrição: dc.descriptionNursing Department Federal University of Maranhão-
Descrição: dc.descriptionDepartment of Periodontology and Implant Dentistry School of Dentistry Federal University of Uberlândia-
Descrição: dc.descriptionDepartment of Basic and Oral Biology Bone Research Lab School of Dentistry of Ribeirão Preto University of São Paulo-
Descrição: dc.descriptionInnovation in Biomaterials Laboratory (iBioM) School of Dentistry Federal University of Goiás-
Descrição: dc.descriptionDepartment of Physiology and Pathology School of Dentistry São Paulo State University-
Descrição: dc.descriptionCNPq: 303599/2014-6-
Idioma: dc.languageen-
Relação: dc.relationJournal of Functional Biomaterials-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectlaser ablation-
Palavras-chave: dc.subjectnanotopography-
Palavras-chave: dc.subjectosseointegration-
Palavras-chave: dc.subjectosteoblast-
Palavras-chave: dc.subjectTi-
Título: dc.titleLaser-Modified Ti Surface Improves Paracrine Osteogenesis by Modulating the Expression of DKK1 in Osteoblasts-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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