Post-mortem muscle proteome of crossbred bulls and steers: Relationships with carcass and meat quality

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.contributorUniversity of Camerino-
Autor(es): dc.contributorInstitut Agro-
Autor(es): dc.creatorSantiago, Bismarck-
Autor(es): dc.creatorBaldassini, Welder-
Autor(es): dc.creatorNeto, Otávio Machado-
Autor(es): dc.creatorChardulo, Luis Artur-
Autor(es): dc.creatorTorres, Rodrigo-
Autor(es): dc.creatorPereira, Guilherme-
Autor(es): dc.creatorCuri, Rogério-
Autor(es): dc.creatorChiaratti, Marcos Roberto-
Autor(es): dc.creatorPadilha, Pedro-
Autor(es): dc.creatorAlessandroni, Laura-
Autor(es): dc.creatorGagaoua, Mohammed-
Data de aceite: dc.date.accessioned2025-08-21T19:28:15Z-
Data de disponibilização: dc.date.available2025-08-21T19:28:15Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-04-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.jprot.2023.104871-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/247046-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/247046-
Descrição: dc.descriptionThis study investigated the skeletal muscle proteome of crossbred bulls and steers with the aim of explaining the differences in carcass and meat quality traits. Therefore, 640 post-weaning Angus-Nellore calves were fed a high-energy diet for a period of 180 days. In the feedlot trial, comparisons of steers (n = 320) and bulls (n = 320) showed lower (P < 0.01) average daily gain (1.38 vs. 1.60 ± 0.05 kg/d), final body weight (547.4 vs. 585.1 ± 9.3 kg), which resulted in lower hot carcass weight (298.4 vs. 333.7 ± 7.7 kg) and ribeye area (68.6 vs. 81.0 ± 2.56 cm2). Steers had higher (P < 0.01) carcass fatness, meat color parameters (L*, a*, b*, chroma (C*), hue (h°)) and lower ultimate pH. Moreover, lower (P < 0.01) Warner-Bratzler shear force (WBSF) were observed in steers compared to bulls (WBSF = 3.68 vs. 4.97 ± 0.08 kg; and 3.19 vs. 4.08 ± 0.08 kg). The proteomic approach using two-dimensional electrophoresis, mass spectrometry and bioinformatics procedures revealed several differentially expressed proteins between steers and bulls (P < 0.05). Interconnected pathways and substantial changes were revealed in biological processes, molecular functions, and cellular components between the post-mortem muscle proteomes of the compared animals. Steers had increased (P < 0.05) abundance of proteins related to energy metabolism (CKM, ALDOA, and GAPDH), and bulls had greater abundance of proteins associated with catabolic (glycolysis) processes (PGM1); oxidative stress (HSP60, HSPA8 and GSTP1); and muscle structure and contraction (TNNI2 and TNNT3). The better carcass (fatness and marbling degree) and meat quality traits (tenderness and color parameters) of steers were associated with higher abundance of key proteins of energy metabolism and lower abundance of enzymes related to catabolic processes, oxidative stress, and proteins of muscle contraction Significance: Sexual condition of cattle is known to be an important factor affecting animal performances and growth as well as the carcass and meat quality traits. The investigation of skeletal muscle proteome help a better understanding of the origin of the differences in quality traits between bulls and steers. The inferior meat quality of bulls was found to be due to the greater expression of proteins associated with primary and catabolic processes, oxidative stress, and muscle contraction. Steers had greater expression of proteins, from which several are known biomarkers of beef quality (mainly tenderness).-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionSchool of Agriculture and Veterinary Sciences (FCAV) São Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionSchool of Veterinary Medicine and Animal Science (FMVZ) São Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionUniversidade Federal de São Carlos (UFSCar) Departamento de Genética e Evolução, São Paulo-
Descrição: dc.descriptionInstitute of Bioscience (IB) São Paulo State University (UNESP) Departamento de Química e Bioquímica, São Paulo-
Descrição: dc.descriptionChemistry Interdisciplinary Project (CHIP) School of Pharmacy University of Camerino, Via Madonna delle Carceri-
Descrição: dc.descriptionPEGASE INRAE Institut Agro-
Descrição: dc.descriptionSchool of Agriculture and Veterinary Sciences (FCAV) São Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionSchool of Veterinary Medicine and Animal Science (FMVZ) São Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionInstitute of Bioscience (IB) São Paulo State University (UNESP) Departamento de Química e Bioquímica, São Paulo-
Descrição: dc.descriptionFAPESP: 2018/00981-5-
Descrição: dc.descriptionFAPESP: 2019/11028-0-
Idioma: dc.languageen-
Relação: dc.relationJournal of Proteomics-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBeef tenderness-
Palavras-chave: dc.subjectFeedlot, meat color, proteomics, biological mechanisms-
Palavras-chave: dc.subjectMass spectrometry-
Palavras-chave: dc.subjectMeat quality-
Palavras-chave: dc.subjectProteome-
Título: dc.titlePost-mortem muscle proteome of crossbred bulls and steers: Relationships with carcass and meat quality-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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