Molecular detection of Babesia spp. and Rickettsia spp. in coatis (Nasua nasua) and associated ticks from midwestern Brazil

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorMato Grosso Do Sul Federal University-
Autor(es): dc.contributorDom Bosco Catholic University-
Autor(es): dc.contributorUniversity of Bari-
Autor(es): dc.contributorBu-Ali Sina University-
Autor(es): dc.creatorPerles, L.-
Autor(es): dc.creatorBarreto, W. T.G.-
Autor(es): dc.creatorde Macedo, G. C.-
Autor(es): dc.creatorCalchi, A. C.-
Autor(es): dc.creatorBezerra-Santos, M.-
Autor(es): dc.creatorMendoza-Roldan, J. A.-
Autor(es): dc.creatorOtranto, D.-
Autor(es): dc.creatorHerrera, H. M.-
Autor(es): dc.creatorBarros-Battesti, D. M.-
Autor(es): dc.creatorMachado, R. Z.-
Autor(es): dc.creatorAndré, M. R.-
Data de aceite: dc.date.accessioned2025-08-21T22:26:44Z-
Data de disponibilização: dc.date.available2025-08-21T22:26:44Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-05-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s00436-023-07815-5-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/246965-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/246965-
Descrição: dc.descriptionProcyonids are reservoirs of many zoonotic infectious diseases, including tick-borne pathogens. The role of coatis (Nasua nasua) in the epidemiology of piroplasmids and Rickettsia has not been fully addressed in Brazil. To molecularly study these agents in coatis and associated ticks, animals were sampled in two urban areas in Midwestern Brazil. Blood (n = 163) and tick (n = 248) DNA samples were screened by PCR assays targeting the 18S rRNA and gltA genes of piroplasmids and Rickettsia spp., respectively. Positive samples were further molecularly tested targeting cox-1, cox-3, β-tubulin, cytB, and hsp70 (piroplasmid) and ompA, ompB, and htrA 17-kDa (Rickettsia spp.) genes, sequenced and phylogenetically analyzed. All coatis’ blood samples were negative for piroplasmids, whereas five pools of ticks (2%) were positive for two different sequences of Babesia spp. The first from Amblyomma sculptum nymphs was close (i.e., ≥ 99% nucleotide identity) to a Babesia sp. previously found in capybaras (Hydrochoerus hydrochaeris); the second from Amblyomma dubitatum nymphs and Amblyomma spp. larvae was identical (100% nucleotide identity) to a Babesia sp. detected in opossums (Didelphis albiventris) and associated ticks. Four samples (0.8%) were positive by PCR to two different Rickettsia spp. sequences, being the first from Amblyomma sp. larva identical to Rickettsia belli and the second from A. dubitatum nymph identical to Rickettsia species from Spotted Fever Group (SFG). The detection of piroplasmids and SFG Rickettsia sp. highlights the importance of Amblyomma spp. in the maintenance of tick-borne agents in urban parks where humans and wild and domestic animals are living in sympatry.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionVector-Borne Bioagents Laboratory (VBBL) Department of Pathology Reproduction and One Health School of Agricultural and Veterinarian Sciences São Paulo State University (Unesp), Via de Acesso Prof. Paulo Donato Castellane, S/N, Zona Rural, SP-
Descrição: dc.descriptionPost-Graduation of Ecology and Conservation Mato Grosso Do Sul Federal University, MS-
Descrição: dc.descriptionLaboratory of Parasitic Biology Environmental Sciences and Farming Sustainability Dom Bosco Catholic University, MS-
Descrição: dc.descriptionDepartment of Veterinary Medicine University of Bari-
Descrição: dc.descriptionFaculty of Veterinary Sciences Bu-Ali Sina University-
Descrição: dc.descriptionVector-Borne Bioagents Laboratory (VBBL) Department of Pathology Reproduction and One Health School of Agricultural and Veterinarian Sciences São Paulo State University (Unesp), Via de Acesso Prof. Paulo Donato Castellane, S/N, Zona Rural, SP-
Descrição: dc.descriptionFAPESP: 2019/15150-4-
Descrição: dc.descriptionFAPESP: 2020/12037-0-
Descrição: dc.descriptionCNPq: 303701/2021-8-
Descrição: dc.descriptionCNPq: 308768/2017-5-
Formato: dc.format1151-1158-
Idioma: dc.languageen-
Relação: dc.relationParasitology Research-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAmblyomma dubitatum-
Palavras-chave: dc.subjectAmblyomma sculptum-
Palavras-chave: dc.subjectBabesia sp-
Palavras-chave: dc.subjectSFG Rickettsia sp-
Palavras-chave: dc.subjectTick-borne pathogens-
Palavras-chave: dc.subjectZoonosis-
Título: dc.titleMolecular detection of Babesia spp. and Rickettsia spp. in coatis (Nasua nasua) and associated ticks from midwestern Brazil-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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