Portable near-infrared spectroscopy: A rapid and accurate blood test for diagnosis of Haemonchus contortus infection and for targeted selective treatment of sheep

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)-
Autor(es): dc.creatorSantos, Isabella B.-
Autor(es): dc.creatorFerreira, Avelardo U.C.-
Autor(es): dc.creatorRabelo, Márcio D.-
Autor(es): dc.creatorAnholeto, Luís Adriano-
Autor(es): dc.creatorSousa, Gustavo A.-
Autor(es): dc.creatorGaínza, Yousmel A.-
Autor(es): dc.creatorFigueiredo, Amanda-
Autor(es): dc.creatorEsteves, Sérgio N.-
Autor(es): dc.creatorChagas, Ana Carolina S.-
Data de aceite: dc.date.accessioned2025-08-21T15:59:59Z-
Data de disponibilização: dc.date.available2025-08-21T15:59:59Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-01-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.ijpara.2022.12.004-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/246782-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/246782-
Descrição: dc.descriptionHaemonchus contortus is the most prevalent and important gastrointestinal nematode (GIN) in small ruminants. Since it reduces the packed cell volume (PCV), causing anemia, early diagnosis can be used for targeted selective treatment (TST) of sheep, reducing antiparasitic drug use and anthelmintic resistance. This study aimed to predict PCV values through near-infrared spectroscopy (NIRS) and to develop a classification and diagnosis model of H. contortus infection using PCV values, eggs per gram of feces (EPG) counts and mean daily weight gain (DWG). A total of 1728 spectra were collected from blood samples of 216 lambs with a portable NIR spectroscope. In parallel, other parameters indicative of infection were measured: PCV by hematocrit, FAffa MAlan CHArt (FAMACHA) scores, EPG and DWG. To evaluate the relationship between NIRS spectra and the evaluated parameters, principal component analysis (PCA) was used for an exploratory analysis, regression by the partial least squares method (PLS) for the prediction of PCV values via NIRS, and PCA linear discriminant analysis (PCA-LDA) as a classification model for diagnosis. The absorption peaks in the NIRS region associated with the excitation of overtones of nitrogen-hydrogen (N-H) functional groups of proteins had a strong impact on the principal components (PCs), indicating that blood proteins, especially hemoglobin, can be estimated by the NIRS technique. The model for predicting PCV by PLS presented a standard error of prediction of 2.53, root-mean-square error of 2.48, and coefficient of determination of 0.84, indicating good correlation between the PCV values predicted by the model and the PCV obtained by hematocrit. The PCA-LDA model presented 93.33% sensitivity and 82.18% accuracy, both higher than those of the FAMACHA method, as was expected for resilient Morada Nova lambs. The multivariate models associated with the NIRS technique reported here can be used in the future as a quick and versatile tool for H. contortus infection diagnosis and TST application in lambs.-
Descrição: dc.descriptionFaculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista, Rod. Prof. Paulo Donato Castellane, São Paulo-
Descrição: dc.descriptionEmbrapa Pecuária Sudeste, Rodovia Washington Luiz, Km 234, SP-
Descrição: dc.descriptionFaculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista, Rod. Prof. Paulo Donato Castellane, São Paulo-
Formato: dc.format119-127-
Idioma: dc.languageen-
Relação: dc.relationInternational Journal for Parasitology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAnemia-
Palavras-chave: dc.subjectDiagnosis-
Palavras-chave: dc.subjectFAMACHA method-
Palavras-chave: dc.subjectLinear Discriminate Analysis-
Palavras-chave: dc.subjectNIRS-
Palavras-chave: dc.subjectParasite control-
Palavras-chave: dc.subjectSmall ruminants-
Título: dc.titlePortable near-infrared spectroscopy: A rapid and accurate blood test for diagnosis of Haemonchus contortus infection and for targeted selective treatment of sheep-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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