Comparison of conventional and contemporary root canal disinfection protocols against bacteria, lipoteichoic acid (LTA), and lipopolysaccharide (LPS)

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Autor(es): dc.contributorUniversity of Maryland-
Autor(es): dc.contributorImam Abdulrahman Bin Faisal University-
Autor(es): dc.contributorPrince Abdulrahman Advanced Dental Institute-
Autor(es): dc.contributorUniversity of Kentucky-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorAlquria, Theeb A.-
Autor(es): dc.creatorAlfirdous, Rayyan A.-
Autor(es): dc.creatorGupta, Swati-
Autor(es): dc.creatorSantamaria, Mauro P.-
Autor(es): dc.creatorSantamaria, Ingrid F.-
Autor(es): dc.creatorGomes, Ana P. M.-
Autor(es): dc.creatorTiradentes, Naiara-
Autor(es): dc.creatorSilva, Eduardo G.-
Autor(es): dc.creatorMartinho, Frederico C.-
Data de aceite: dc.date.accessioned2025-08-21T17:51:16Z-
Data de disponibilização: dc.date.available2025-08-21T17:51:16Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1038/s41598-022-26855-y-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/246697-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/246697-
Descrição: dc.descriptionThis study devised a dual-species biofilm model to investigate bacteria, lipoteichoic acid (LTA), and lipopolysaccharide (LPS) simultaneously, and compared the efficacy of conventional and contemporary disinfection protocols. Seventy single-rooted mandibular premolars were included. Fourteen teeth were negative control, and 56 teeth were infected with 3-week-old E. faecalis and E. coli GFP biofilm. Fourteen/56 teeth were positive control, with seven teeth processed for CLSM analysis and seven teeth sampled with paper points (PPs) and cryogenically ground for bacterial, LTA, and LPS analyses. Forty-two teeth were randomly divided into three groups: GWS (GentleWave system) + MIT (minimally invasive technique), PUI (passive ultrasonic irrigation) + CIT (conventional instrumentation technique), and XP-EF (XP-endo Finisher) + CIT (All, n = 14). Samples were collected before (s1) and after disinfection (s2) with PPs and after cryogenically ground (s3). CFUs were counted, and LTA and LPS were quantified with LTA-ELISA and LAL assay, respectively. XP-EF was as effective as PUI (p > 0.05). GWS + MIT was the most effective disinfection protocol against bacteria, LTA, and LPS (p < 0.05). In conclusion, PUI, XP-EF, and GWS were highly effective against bacteria, LTA, and LPS, with GWS being the most effective.-
Descrição: dc.descriptionDepartment of Advanced Oral Sciences and Therapeutics School of Dentistry University of Maryland-
Descrição: dc.descriptionDepartment of Restorative Dental Science College of Dentistry Imam Abdulrahman Bin Faisal University-
Descrição: dc.descriptionPrince Abdulrahman Advanced Dental Institute-
Descrição: dc.descriptionCollege of Dentistry University of Kentucky-
Descrição: dc.descriptionDepartment of General Dentistry School of Dentistry University of Maryland-
Descrição: dc.descriptionEndodontic Division Department of Restorative Dentistry Institute of Science and Technology São Paulo State University (Unesp)-
Descrição: dc.descriptionDepartment of Social and Pediatric Dentistry Institute of Science and Technology Institute of Science and Technology São Paulo State University (Unesp)-
Descrição: dc.descriptionEndodontic Division Department of Restorative Dentistry Institute of Science and Technology São Paulo State University (Unesp)-
Descrição: dc.descriptionDepartment of Social and Pediatric Dentistry Institute of Science and Technology Institute of Science and Technology São Paulo State University (Unesp)-
Idioma: dc.languageen-
Relação: dc.relationScientific Reports-
???dc.source???: dc.sourceScopus-
Título: dc.titleComparison of conventional and contemporary root canal disinfection protocols against bacteria, lipoteichoic acid (LTA), and lipopolysaccharide (LPS)-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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