Expanding the Functionality of an Autoinduction Device for Repression of Gene Expression in Bacillus subtilis

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade Federal do ABC (UFABC)-
Autor(es): dc.creatorSilva, Bruna F.-
Autor(es): dc.creatorCorrêa, Graciely G.-
Autor(es): dc.creatorZocca, Vitória F. B.-
Autor(es): dc.creatorPicheli, Flavio P.-
Autor(es): dc.creatorLins, Milca R. C. R.-
Autor(es): dc.creatorPedrolli, Danielle B.-
Data de aceite: dc.date.accessioned2025-08-21T23:21:45Z-
Data de disponibilização: dc.date.available2025-08-21T23:21:45Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2022-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/ijms24010084-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/246629-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/246629-
Descrição: dc.descriptionAutonomous control of gene expression through engineered quorum-sensing processes is broadly applicable to biosynthetic pathways, including simultaneous control of different genes. It is also a powerful tool for balancing growth and production. We had previously engineered a modular autoinduction device for the control of gene expression in B. subtilis. Now, we expand its functionality to repress gene expression autonomously. The engineered R8 promoter responds to AHL accumulation in the culture medium. In a riboflavin-producing strain, the AHL-Lux complex exerts 5-fold repression on the R8-driven expression of the flavokinase/FAD synthetase gene ribC, resulting in a higher titer of the vitamin. We engineered a strain able to autonomously induce and repress different genes simultaneously, demonstrating the potential of the device for use in metabolic engineering.-
Descrição: dc.descriptionDepartment of Bioprocess Engineering and Biotechnology School of Pharmaceutical Sciences Universidade Estadual Paulista (UNESP), Rodovia Araraquara-Jau km 1-
Descrição: dc.descriptionCenter for Natural and Human Sciences Federal University of ABC (UFABC), Campus Santo André, Avenida dos Estados 5001-
Descrição: dc.descriptionDepartment of Bioprocess Engineering and Biotechnology School of Pharmaceutical Sciences Universidade Estadual Paulista (UNESP), Rodovia Araraquara-Jau km 1-
Idioma: dc.languageen-
Relação: dc.relationInternational Journal of Molecular Sciences-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectautoinduction-
Palavras-chave: dc.subjectautonomous control-
Palavras-chave: dc.subjectBacillus subtilis-
Palavras-chave: dc.subjectdynamic regulation-
Palavras-chave: dc.subjectquorum-sensing-
Palavras-chave: dc.subjectriboflavin-
Título: dc.titleExpanding the Functionality of an Autoinduction Device for Repression of Gene Expression in Bacillus subtilis-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.