Firocoxib as a Potential Neoadjuvant Treatment in Canine Patients with Triple-Negative Mammary Gland Tumors

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversity of Milan-
Autor(es): dc.contributorFederal University of Fronteira Sul-UFFS-
Autor(es): dc.contributorCEVEPAT Veterinary Diagnostic Laboratory-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorPaulista University-UNIP-
Autor(es): dc.creatorBrandi, Andressa-
Autor(es): dc.creatorde Faria Lainetti, Patricia-
Autor(es): dc.creatorElias, Fabiana-
Autor(es): dc.creatorRodrigues, Marcela Marcondes Pinto-
Autor(es): dc.creatorFagundes Moraes, Livia-
Autor(es): dc.creatorLaufer-Amorim, Renée-
Autor(es): dc.creatorde Camargo, Laíza Sartori-
Autor(es): dc.creatorSalles Gomes, Cristina de Oliveira Massoco-
Autor(es): dc.creatorFonseca-Alves, Carlos Eduardo-
Data de aceite: dc.date.accessioned2025-08-21T17:44:47Z-
Data de disponibilização: dc.date.available2025-08-21T17:44:47Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2022-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/ani13010060-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/246614-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/246614-
Descrição: dc.descriptionThis study aimed to investigate the pro-apoptotic effects of NSAID (Previcox®) in vitro and in vivo. Two CMT cell lines, one from the primary tumor and one from bone metastasis, were treated with firocoxib and MTT assay was performed to determine the half-maximal inhibitory concentration (IC50) value. The firocoxib IC50 for the cell lines UNESP-CM5 and UNESP-MM1 were 25.21 µM and 27.41 µM, respectively. The cell lines were then treated with the respective firocoxib IC50 concentrations and annexin V/propidium iodide (PI) assay was performed, to detect the induction of apoptosis in both cells (Annexin+/PI+). We conducted an in vivo study involving female dogs affected by CMT and divided them into control and treatment groups. For both groups, a biopsy was performed on day 0 (D0) and a mastectomy was performed on day 14 (D14). In the treatment group, after biopsy on D0, the patients received Previcox® 5 mg/kg PO once a day until mastectomy was performed on D14. COX-2/caspase-3 double immunostaining was performed on samples from D0 and D14, revealing no difference in the control group. In contrast, in the treatment group Previcox® increased the number of COX-2 positive apoptotic cells. Therefore, firocoxib can induce apoptosis in CMT cells in vitro and in vivo, and Previcox® can be a potential neoadjuvant treatment for patients with mammary cancer.-
Descrição: dc.descriptionDepartment of Veterinary Surgery and Animal Reproduction São Paulo State University-UNESP-
Descrição: dc.descriptionDepartment of Veterinary Medicine and Animal Science University of Milan, Via dell’ Università 6-
Descrição: dc.descriptionVeterinary School Federal University of Fronteira Sul-UFFS-
Descrição: dc.descriptionCEVEPAT Veterinary Diagnostic Laboratory-
Descrição: dc.descriptionDepartment of Veterinary Clinic Sao Paulo State University-UNESP-
Descrição: dc.descriptionDepartment of Pathology University of São Paulo-USP-
Descrição: dc.descriptionInstitute of Health Sciences Paulista University-UNIP-
Descrição: dc.descriptionDepartment of Veterinary Surgery and Animal Reproduction São Paulo State University-UNESP-
Descrição: dc.descriptionDepartment of Veterinary Clinic Sao Paulo State University-UNESP-
Idioma: dc.languageen-
Relação: dc.relationAnimals-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectcaspase-3-
Palavras-chave: dc.subjectchemotherapy-
Palavras-chave: dc.subjectcyclooxygenase-2-
Palavras-chave: dc.subjectnonsteroidal anti-inflammatory drug-
Título: dc.titleFirocoxib as a Potential Neoadjuvant Treatment in Canine Patients with Triple-Negative Mammary Gland Tumors-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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