Potential Cytoprotective and Regulatory Effects of Ergothioneine on Gene Expression of Proteins Involved in Erythroid Adaptation Mechanisms and Redox Pathways in K562 Cells

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.contributorFundação Universidade Regional de Blumenau (FURB)-
Autor(es): dc.contributorUniversidade Federal de Mato Grosso do Sul (UFMS)-
Autor(es): dc.creatorBernardo, Victoria Simões-
Autor(es): dc.creatorTorres, Flaviene Felix-
Autor(es): dc.creatorde Paula, Carla Peres-
Autor(es): dc.creatorda Silva, João Pedro Maia de Oliveira-
Autor(es): dc.creatorde Almeida, Eduardo Alves-
Autor(es): dc.creatorda Cunha, Anderson Ferreira-
Autor(es): dc.creatorda Silva, Danilo Grünig Humberto-
Data de aceite: dc.date.accessioned2025-08-21T22:13:45Z-
Data de disponibilização: dc.date.available2025-08-21T22:13:45Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2022-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/genes13122368-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/246515-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/246515-
Descrição: dc.descriptionThis study aimed to establish the importance of ergothioneine (ERT) in the erythroid adaptation mechanisms by appraising the expression levels of redox-related genes associated with the PI3K/AKT/FoxO3 and Nrf2-ARE pathways using K562 cells induced to erythroid differentiation and H2O2-oxidative stress. Cell viability and gene expression were evaluated. Two concentrations of ERT were assessed, 1 nM (C1) and 100 µM (C2), with and without stress induction (100 µM H2O2). Assessments were made in three periods of the cellular differentiation process (D0, D2, and D4). The C1 treatment promoted the induction of FOXO3 (D0 and 2), PSMB5, and 6 expressions (D4); C1 + H2O2 treatment showed the highest levels of NRF2 transcripts, KEAP1 (D0), YWHAQ (D2 and 4), PSMB5 (D2) and PSMB6 (D4); and C2 + H2O2 (D2) an increase in FOXO3 and MST1 expression, with a decrease of YWHAQ and NRF2 was observed. in C2 + H2O2 (D2) an increase in FOXO3 and MST1, with a decrease in YWHAQ and NRF2 was observed All ERT treatments increased gamma-globin expression. Statistical multivariate analyzes highlighted that the Nrf2-ARE pathway presented a greater contribution in the production of PRDX1, SOD1, CAT, and PSBM5 mRNAs, whereas the PI3K/AKT/FoxO3 pathway was associated with the PRDX2 and TRX transcripts. In conclusion, ERT presented a cytoprotective action through Nrf2 and FoxO3, with the latter seeming to contribute to erythroid proliferation/differentiation.-
Descrição: dc.descriptionDepartment of Biology Universidade Estadual Paulista (UNESP), SP-
Descrição: dc.descriptionDepartment of Genetics and Evolution Universidade Federal de São Carlos (UFSCar), SP-
Descrição: dc.descriptionDepartment of Natural Sciences Fundação Universidade Regional de Blumenau (FURB), SC-
Descrição: dc.descriptionUniversidade Federal de Mato Grosso do Sul (CPTL/UFMS), Campus de Três Lagoas, MS-
Descrição: dc.descriptionDepartment of Biology Universidade Estadual Paulista (UNESP), SP-
Idioma: dc.languageen-
Relação: dc.relationGenes-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectantioxidants-
Palavras-chave: dc.subjectproteasome-
Palavras-chave: dc.subjectredox adaptation-
Palavras-chave: dc.subjecttherapeutic agent-
Título: dc.titlePotential Cytoprotective and Regulatory Effects of Ergothioneine on Gene Expression of Proteins Involved in Erythroid Adaptation Mechanisms and Redox Pathways in K562 Cells-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.