Immunosuppressive, antimicrobial and insecticidal activities of inhibitor cystine knot peptides produced by teratocytes of the endoparasitoid wasp Cotesia flavipes (Hymenoptera: Braconidae)

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorThe University of Queensland-
Autor(es): dc.contributorQueensland-
Autor(es): dc.creatorPinto, Ciro P. G.-
Autor(es): dc.creatorWalker, Andrew A.-
Autor(es): dc.creatorKing, Glenn F.-
Autor(es): dc.creatorRossi, Guilherme D.-
Data de aceite: dc.date.accessioned2025-08-21T17:24:12Z-
Data de disponibilização: dc.date.available2025-08-21T17:24:12Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2021-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1111/1744-7917.13154-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/246489-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/246489-
Descrição: dc.descriptionTeratocytes are specialized cells released by parasitoid wasps into their hosts. They are known for producing regulatory molecules that aid the development of immature parasitoids. We have recently reported the primary structures of cystine-rich peptides, including some containing inhibitor cystine knot (ICK) motifs, produced by teratocytes of the parasitoid Cotesia flavipes (Hymenoptera: Braconidae). ICKs are known for their stability and diverse biological functions. In this study, we produced four putative ICK peptides from the teratocytes of C. flavipes using solid-phase peptide synthesis or recombinant expression in E. coli, and investigated their functions on host immune modulation as well their potential to impair the development of two lepidopterans after ingestion of the peptides. In addition, the peptides were assayed against pathogens and human cells. The peptides did not influence total hemocyte count but suppressed cellular immunity, detectable as a reduction of hemocyte encapsulation (CftICK-I, CftICK-II, CftICK-III) and spread indexes (CftICK-IV) in the host. None of the peptides influenced the activities of prophenoloxidase and phenoloxidase in the hemolymph of larval Diatraea saccharalis (Lepidoptera: Crambidae). CftICK-I and CftICK-II with previously unknown function showed antifungal activity against Candida albicans but were non-toxic to human cells. CftICK-I, CftICK-II, and CftICK-III increased larval mortality and reduced leaf consumption of D. saccharalis, a permissive host for C. flavipes. The CftICK-III also increased larval mortality and reduced leaf consumption of Spodoptera frugiperda (Lepidoptera: Noctuidae), a non-permissive host for C. flavipes. This study highlights biological functions and biotechnological potential of ICK peptides from the teratocytes of C. flavipes.-
Descrição: dc.descriptionSchool of Agricultural and Veterinarian Sciences São Paulo State University (UNESP)-
Descrição: dc.descriptionInstitute for Molecular Bioscience The University of Queensland-
Descrição: dc.descriptionAustralian Research Council Centre of Excellence for Innovations in Peptide and Protein Science The University of Queensland Queensland-
Descrição: dc.descriptionSchool of Agricultural and Veterinarian Sciences São Paulo State University (UNESP)-
Idioma: dc.languageen-
Relação: dc.relationInsect Science-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectbiocontrol-
Palavras-chave: dc.subjectencapsulation-
Palavras-chave: dc.subjecthost regulation-
Palavras-chave: dc.subjectHymenoptera-
Palavras-chave: dc.subjectparasitoid-
Palavras-chave: dc.subjectphenoloxidase-
Título: dc.titleImmunosuppressive, antimicrobial and insecticidal activities of inhibitor cystine knot peptides produced by teratocytes of the endoparasitoid wasp Cotesia flavipes (Hymenoptera: Braconidae)-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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