Antioxidant and Neuroprotective Effects of the First Tryptophyllin Found in Snake Venom (Bothrops moojeni)

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Autor(es): dc.contributorUniversity of Brasilia-
Autor(es): dc.contributorParnaiba Delta Federal University-
Autor(es): dc.contributorUniversity of Brasília-
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversity of Porto-
Autor(es): dc.contributorUniversity of Lincoln-
Autor(es): dc.creatorDematei, Anderson-
Autor(es): dc.creatorCosta, Samuel Ribeiro-
Autor(es): dc.creatorMoreira, Daniel C.-
Autor(es): dc.creatorBarbosa, Eder Alves-
Autor(es): dc.creatorFriaça Albuquerque, Lucas F.-
Autor(es): dc.creatorVasconcelos, Andreanne G.-
Autor(es): dc.creatorNascimento, Tiago-
Autor(es): dc.creatorSilva, Pedro Costa-
Autor(es): dc.creatorSilva-Carvalho, Amandda É.-
Autor(es): dc.creatorSaldanha-Araújo, Felipe-
Autor(es): dc.creatorSilva Mancini, Mariana Camargo-
Autor(es): dc.creatorSaboia Ponte, Luis Gustavo-
Autor(es): dc.creatorNeves Bezerra, Rosangela Maria-
Autor(es): dc.creatorSimabuco, Fernando Moreira-
Autor(es): dc.creatorBatagin-Neto, Augusto-
Autor(es): dc.creatorBrand, Guilherme-
Autor(es): dc.creatorBorges, Tatiana Karla S.-
Autor(es): dc.creatorEaton, Peter-
Autor(es): dc.creatorLeite, José Roberto S. A.-
Data de aceite: dc.date.accessioned2025-08-21T21:57:53Z-
Data de disponibilização: dc.date.available2025-08-21T21:57:53Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2022-12-22-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1021/acs.jnatprod.2c00304-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/246476-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/246476-
Descrição: dc.descriptionIn this study, we report the isolation, characterization, and synthesis of the peptide BmT-2 belonging to the tryptophyllins family, isolated from the venom of the snake Bothrops moojeni. This is the first time a tryptophyllin is identified in snake venom. We tested whether BmT-2 had cytotoxic effects and antioxidant activity in a set of experiments that included both in vitro and cell-based assays. BmT-2 presented a radical scavenging activity toward ABTS• and AAPH-derived radicals. BmT-2 protected fluorescein, DNA molecules, and human red blood cells (RBCs) from free radicals generated by the thermal decomposition of AAPH. The novel tryptophyllin was not toxic in cell viability tests, where it (up to 0.4 mg/mL) did not cause hemolysis of human RBCs and did not cause significant loss of cell viability, showing a CC50 > 1.5 mM for cytotoxic effects against SK-N-BE(2) neuroblastoma cells. BmT-2 prevented the arsenite-induced upregulation of Nrf2 in Neuro-2a neuroblasts and the phorbol myristate acetate-induced overgeneration of reactive oxygen species and reactive nitrogen species in SK-N-BE(2) neuroblastoma cells. Electronic structure calculations and full atomistic reactive molecular dynamics simulations revealed the relevant contribution of aromatic residues in BmT-2 to its antioxidant properties. Our study presents a novel peptide classified into the family of the tryptophyllins, which has been reported exclusively in amphibians. Despite the promising results on its antioxidant activity and low cytotoxicity, the mechanisms of action of BmT-2 still need to be further elucidated.-
Descrição: dc.descriptionCenter for Tropical Medicine (NMT) Faculty of Medicine University of Brasilia-
Descrição: dc.descriptionResearch Center in Morphology and Applied Immunology (NuPMIA) Faculty of Medicine University of Brasilia-
Descrição: dc.descriptionLaboratory for the Synthesis and Analysis of Biomolecules (LSAB) Institute of Chemistry University of Brasilia-
Descrição: dc.descriptionResearch Center on Biodiversity and Biotechnology (Biotec) Parnaiba Delta Federal University-
Descrição: dc.descriptionLaboratory of Hematology and Stem Cells (LHCT) Faculty of Health Sciences University of Brasília-
Descrição: dc.descriptionMultidisciplinary Laboratory of Food and Health (LabMAS) School of Applied Sciences University of Campinas-
Descrição: dc.descriptionInstitute of Science and Engineering São Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionLAQV/REQUIMTE Department of Chemistry and Biochemistry Faculty of Sciences University of Porto-
Descrição: dc.descriptionThe Bridge Joseph Banks Laboratories School of Chemistry University of Lincoln-
Descrição: dc.descriptionInstitute of Science and Engineering São Paulo State University (UNESP), São Paulo-
Formato: dc.format2695-2705-
Idioma: dc.languageen-
Relação: dc.relationJournal of Natural Products-
???dc.source???: dc.sourceScopus-
Título: dc.titleAntioxidant and Neuroprotective Effects of the First Tryptophyllin Found in Snake Venom (Bothrops moojeni)-
Tipo de arquivo: dc.typelivro digital-
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