Molecular detection of equid herpesvirus in bronchoalveolar lavage fluid from asymptomatic horses in Southern Brazil

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Autor(es): dc.contributorUniversidade Federal do Paraná (UFPR)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorVirginia Tech-
Autor(es): dc.contributorPurdue University-
Autor(es): dc.contributorMolecular Diagnostics-
Autor(es): dc.creatorFinger, Mariane Angélica-
Autor(es): dc.creatorBiava, Janaina Socolovski-
Autor(es): dc.creatorDornbusch, Peterson Triches-
Autor(es): dc.creatorPerotta, João Henrique-
Autor(es): dc.creatorUllmann, Leila Sabrina-
Autor(es): dc.creatorda SiIva Serpa, Priscila Beatriz-
Autor(es): dc.creatorKmetiuk, Louise Bach-
Autor(es): dc.creatordos Santos, Andrea Pires-
Autor(es): dc.creatorBiondo, Alexander Welker-
Autor(es): dc.creatorLeutenegger, Christian-
Autor(es): dc.creatorde Barros Filho, Ivan Roque-
Data de aceite: dc.date.accessioned2025-08-21T23:25:46Z-
Data de disponibilização: dc.date.available2025-08-21T23:25:46Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2022-10-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.14202/vetworld.2022.2597-2602-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/246434-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/246434-
Descrição: dc.descriptionBackground and Aim: Molecular approaches to diagnose respiratory viruses have provided an opportunity for early and subclinical pathogen detection, particularly in samples from the upper respiratory tract. This study aimed to investigate the presence of herpesviruses, particularly equid herpesvirus (EHV)-2 and EHV-5, in samples from the lower respiratory tract of healthy racehorses from Southern Brazil. Materials and Methods: Samples from the lower respiratory tract (i.e., bronchoalveolar lavage fluid [BALF]) were assessed by video endoscopy, cytological evaluation of BALF, and tracheal aspirates (TA), along with quantitative polymerase chain reaction (qPCR), to detect equine herpesvirus infection in the lower respiratory tract samples and compare corresponding cytological and endoscopic findings. Results: At least one abnormality per horse during endoscopy examination was observed, including, but not limited to, mucous secretion in the airways and pharyngeal lymphoid hyperplasia. The presence of EHV-2 and/or EHV-5 was detected by qPCR in 3/10 animals. One horse was positive for EHV-2 alone, one for EHV-5 alone, and one for both. Conclusion: To the authors' knowledge, this is the first molecular detection of EHV-2 and EHV-5 in Brazilian thoroughbred horses. These findings may provide new insights into the epidemiology of EHV-2 and EHV-5 in Brazilian horses, evidencing the importance of the molecular investigation, early detection, and prevention of respiratory diseases.-
Descrição: dc.descriptionGrayson-Jockey Club Research Foundation-
Descrição: dc.descriptionDepartment of Veterinary Medicine UFPR - Federal University of Paraná, Rua dos Funcionários, 1540, PR-
Descrição: dc.descriptionDepartment of Animal Science College of Agriculture “Luiz de Queiroz” University of São Paulo, SP-
Descrição: dc.descriptionBiotechnology Institute Sao Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Biomedical Sciences and Pathobiology Virginia-Maryland College of Veterinary Medicine Virginia Tech-
Descrição: dc.descriptionDepartment of Comparative Pathobiology Purdue University-
Descrição: dc.descriptionAntech Diagnostics Molecular Diagnostics-
Descrição: dc.descriptionBiotechnology Institute Sao Paulo State University (UNESP), SP-
Formato: dc.format2597-2602-
Idioma: dc.languageen-
Relação: dc.relationVeterinary World-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectbronchoalveolar lavage fluid-
Palavras-chave: dc.subjectendoscopy-
Palavras-chave: dc.subjectequine-
Palavras-chave: dc.subjectgammaherpesvirus-
Palavras-chave: dc.subjectpolymerase chain reaction-
Palavras-chave: dc.subjecttracheal wash-
Título: dc.titleMolecular detection of equid herpesvirus in bronchoalveolar lavage fluid from asymptomatic horses in Southern Brazil-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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