Single-cell-resolution transcriptome map revealed novel genes involved in testicular germ cell progression and somatic cells specification in Chinese tongue sole with sex reversal

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Autor(es): dc.contributorChinese Academy of Fishery Sciences-
Autor(es): dc.contributorQingdao National Laboratory for Marine Science and Technology-
Autor(es): dc.contributorShanghai Ocean University-
Autor(es): dc.contributorBGI-
Autor(es): dc.contributorBGI-Shenzhen-
Autor(es): dc.contributorNingbo University-
Autor(es): dc.contributorDalian Ocean University-
Autor(es): dc.contributorShandong Normal University-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorSpanish National Research Council (CSIC)-
Autor(es): dc.creatorWang, Hong-Yan-
Autor(es): dc.creatorLiu, Xiang-
Autor(es): dc.creatorChen, Jian-Yang-
Autor(es): dc.creatorHuang, Yingyi-
Autor(es): dc.creatorLu, Yifang-
Autor(es): dc.creatorTan, Fujian-
Autor(es): dc.creatorLiu, Qun-
Autor(es): dc.creatorYang, Mingming-
Autor(es): dc.creatorLi, Shuo-
Autor(es): dc.creatorZhang, Xianghui-
Autor(es): dc.creatorQin, Yating-
Autor(es): dc.creatorMa, Wenxiu-
Autor(es): dc.creatorYang, Yingming-
Autor(es): dc.creatorMeng, Liang-
Autor(es): dc.creatorLiu, Kaiqiang-
Autor(es): dc.creatorWang, Qian-
Autor(es): dc.creatorFan, Guangyi-
Autor(es): dc.creatorNóbrega, Rafael H.-
Autor(es): dc.creatorLiu, Shanshan-
Autor(es): dc.creatorPiferrer, Francesc-
Autor(es): dc.creatorShao, Changwei-
Data de aceite: dc.date.accessioned2025-08-21T22:39:19Z-
Data de disponibilização: dc.date.available2025-08-21T22:39:19Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-05-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s11427-021-2236-4-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/246383-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/246383-
Descrição: dc.descriptionFemale-to-male sex reversals (pseudomales) are common in lower vertebrates and have been found in natural populations, which is a concern under rapid changes in environmental conditions. Pseudomales can exhibit altered spermatogenesis. However, the regulatory mechanisms underlying pseudomale spermatogenesis remain unclear. Here, we characterized spermatogenesis in Chinese tongue sole (Cynoglossus semilaevis), a species with genetic and environmental sex determination, based on a high-resolution single-cell RNA-seq atlas of cells derived from the testes of genotypic males and pseudomales. We identified five germ cell types and six somatic cell types and obtained a single-cell atlas of dynamic changes in gene expression during spermatogenesis in Chinese tongue sole, including alterations in pseudomales. We detected decreased levels of Ca2+ signaling pathway-related genes in spermatogonia, insufficient meiotic initiation in spermatocytes, and a malfunction of somatic niche cells in pseudomales. However, a cluster of CaSR genes and MAPK signaling factors were upregulated in undifferentiated spermatogonia of pseudomales. Additionally, we revealed that Z chromosome-specific genes, such as piwil2, dhx37, and ehmt1, were important for spermatogenesis. These results improve our understanding of reproduction after female-to-male sex-reversal and provide new insights into the adaptability of reproductive strategies in lower vertebrates.-
Descrição: dc.descriptionKey Lab of Sustainable Development of Marine Fisheries Ministry of Agriculture and Rural Affairs Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences-
Descrição: dc.descriptionLaboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and Technology-
Descrição: dc.descriptionCollege of Fisheries and Life Science Shanghai Ocean University-
Descrição: dc.descriptionBGI Research-Qingdao BGI-
Descrição: dc.descriptionBGI-Shenzhen-
Descrição: dc.descriptionSchool of Marine Sciences Ningbo University-
Descrição: dc.descriptionCollege of Marine Technology and Environment Dalian Ocean University-
Descrição: dc.descriptionQingdao-Europe Advanced Institute for Life Sciences BGI-Shenzhen-
Descrição: dc.descriptionCollege of Life Sciences Shandong Normal University-
Descrição: dc.descriptionReproductive and Molecular Biology Group Department of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionInstitut de Ciències del Mar (ICM) Spanish National Research Council (CSIC)-
Descrição: dc.descriptionReproductive and Molecular Biology Group Department of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP), São Paulo-
Formato: dc.format1151-1169-
Idioma: dc.languageen-
Relação: dc.relationScience China Life Sciences-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCynoglossus semilaevis-
Palavras-chave: dc.subjectsex reversal-
Palavras-chave: dc.subjectsingle-cell transcriptome-
Palavras-chave: dc.subjectspermatogenesis-
Palavras-chave: dc.subjectspermatogonia-
Título: dc.titleSingle-cell-resolution transcriptome map revealed novel genes involved in testicular germ cell progression and somatic cells specification in Chinese tongue sole with sex reversal-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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