Antimicrobial photodynamic therapy as an adjunctive treatment to ultrasound for the dentin caries-like lesion removal

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversity of Araraquara - UNIARA-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorde Melo, Priscila Borges Gobbo-
Autor(es): dc.creatorBesegato, João Felipe-
Autor(es): dc.creatorde Abreu Bernardi, Adilson César-
Autor(es): dc.creatorBagnato, Vanderlei Salvador-
Autor(es): dc.creatorde Souza Rastelli, Alessandra Nara-
Data de aceite: dc.date.accessioned2025-08-21T15:17:30Z-
Data de disponibilização: dc.date.available2025-08-21T15:17:30Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2022-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.pdpdt.2022.103148-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/246195-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/246195-
Descrição: dc.descriptionObjective: To evaluate in vitro the efficacy of ultrasound device to remove caries-like dentin and the curcumin-mediated antimicrobial photodynamic therapy (aPDT) to decontaminate the affected dentin. Methods: Bovine dentin specimens (n = 173) of 4 × 4 × 2 mm were first submitted to Knoop surface microhardness to standardize the specimens (29 ± 3 KHN). Artificial caries lesion was induced by Streptococcus mutans strain by biological model for 7 days. Infected dentin was removed (1 min) with the following techniques: dentin excavator, bur at low-speed rotation and ultrasound device. After that, aPDT application was performed using blue LED under 460 nm. Polarized light microscopy (PLM), removal rate (n = 10), cross-sectional microhardness (n = 10), colony forming units per milliliter (CFU) (n = 9) and confocal laser microscopy (CM) (n = 2) were performed. ANOVA with Welch correction, post-hoc Games-Howell and two-way ANOVA followed by Tukey's post-hoc tests were used. Results: PLM confirmed the caries lesion formation with a depth of ∼147.9 µm. Groups treated with ultrasound showed lower removal rate (p = 0.001). Regardless of the treatment, the microhardness values increased as function of depth (p ≤ 0.05). Carbide bur showed the highest microhardness value, followed by ultrasound and excavator. CFU and CM showed a significant reduction in S. mutans after aPDT application. Conclusion: Ultrasound was efficient, since it removed infected dentin, preserving the affected dentin and aPDT can be used as a complementary therapy to decontaminate the affected dentin. Clinical significance: Ultrasound device may help the clinician to remove dentin caries-like lesions since it is a conservative technique and provided the removal of infected dentin, preserving the affected dentin. aPDT application may be used as a complimentary technique to promote antibacterial effect and possibly minimize the risk of secondary caries.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionUniversidade de São Paulo-
Descrição: dc.descriptionDepartment of Restorative Dentistry School of Dentistry Sao Paulo State University – UNESP, 1680 Humaitá Street, 3rd floor, SP-
Descrição: dc.descriptionDepartment of Biology and Health Sciences University of Araraquara - UNIARA, SP-
Descrição: dc.descriptionDepartment of Physics and Materials Science Physics Institute of Sao Carlos-IFSC University of Sao Paulo – USP, SP-
Descrição: dc.descriptionDepartment of Restorative Dentistry School of Dentistry Sao Paulo State University – UNESP, 1680 Humaitá Street, 3rd floor, SP-
Descrição: dc.descriptionCAPES: 001-
Descrição: dc.descriptionUniversidade de São Paulo: 07276-1/2013-
Descrição: dc.descriptionCAPES: 88882.432525/2019-
Idioma: dc.languageen-
Relação: dc.relationPhotodiagnosis and Photodynamic Therapy-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAntimicrobial photodynamic therapy-
Palavras-chave: dc.subjectBiofilm-
Palavras-chave: dc.subjectDental caries-
Palavras-chave: dc.subjectStreptococcus mutans-
Palavras-chave: dc.subjectUltrasound-
Título: dc.titleAntimicrobial photodynamic therapy as an adjunctive treatment to ultrasound for the dentin caries-like lesion removal-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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