TiO2 nanoparticles added to dense bovine hydroxyapatite bioceramics increase human osteoblast mineralization activity

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorFernandes, Paulo Henrique Martins-
Autor(es): dc.creatorBordini, Ester Alves Ferreira-
Autor(es): dc.creatorCassiano, Fernanda Balestrero-
Autor(es): dc.creatorde Azevedo-Silva, Lucas José-
Autor(es): dc.creatorFerrairo, Brunna Mota-
Autor(es): dc.creatorLisboa-Filho, Paulo Noronha-
Autor(es): dc.creatorFortulan, Carlos Alberto-
Autor(es): dc.creatorSoares dos Passos, Diana Gabriela-
Autor(es): dc.creatorBorges, Ana Flávia Sanches-
Data de aceite: dc.date.accessioned2025-08-21T20:12:22Z-
Data de disponibilização: dc.date.available2025-08-21T20:12:22Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2022-10-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.dental.2022.08.007-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/245978-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/245978-
Descrição: dc.descriptionObjectives: This study evaluated the effect of TiO2 nanoparticles + dense hydroxyapatite (HA) on human osteoblast cells (SAOS-2). Methods: Particulate bovine HA powder with or without the addition of either 5 or 8 % TiO2 (HA, HA/TiO2Np5 % or HA/TiO2Np8 %) were pressed into disks (Ø = 12.5 mm; thickness = 1.3 mm) uniaxially (100 MPa) and isostatically (200 MPa/1 min) and sintered at 1300 °C. Y-TZP disks were used as control. The following tests were performed: Scanning Electron Microscopy and Dispersive Energy Spectroscopy (SEM/EDS), Atomic Force Microscopy (AFM), cell viability assay (Alamar Blue-AB) and mineralized matrix deposition (Alizarin Red-AR). AB and AR data were submitted to 2-way ANOVA/Tukey tests and ANOVA/Tukey tests, respectively. Results: SEM revealed that the surface of HA/TiO2Np5% resembles DPBHA surface, but also contains smaller granules. HA/TiO2Np8% characteristics resembles HA/TiO2Np5% surface, but with irregular topography. Y-TZP showed a typical oxide ceramic surface pattern. EDS revealed Ca, O, and P in all samples. C, O, and Zr appeared in Y-TZP samples. AFM data corroborates SEM analysis. AB test revealed excellent cellular viability for HA/TiO2Np5% group. AR test showed that all groups containing TiO2np had more mineralized matrix deposition than all other groups, with statistically differences between HA/TiO2Np8% and HA cultivated in non-osteogenic medium. Culture in osteogenic medium exhibited much more mineralized matrix deposition by TiO2np groups. Significance: In conclusion, the addition of TiO2np showed chemical, superficial, and biological changes in the reinforced materials. HA/TiO2Np5% showed the best results for cell viability and HA/TiO2Np8% for mineralized matrix deposition.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Operative Dentistry Endodontics and Dental Materials Bauru School of Dentistry University of São Paulo, SP-
Descrição: dc.descriptionDepartment of Prosthodontics and Periodontics Bauru School of Dentistry University of São Paulo, SP-
Descrição: dc.descriptionDepartment of Physics School of Sciences São Paulo State University, SP-
Descrição: dc.descriptionDepartment of Mechanical Engineering São Carlos School of Engineering University of São Paulo, SP-
Descrição: dc.descriptionDepartment of Physics School of Sciences São Paulo State University, SP-
Descrição: dc.descriptionCAPES: 001-
Descrição: dc.descriptionCNPq: 154705/2015–2-
Descrição: dc.descriptionFAPESP: 2013/07296–2-
Descrição: dc.descriptionFAPESP: 2016/15674-5-
Descrição: dc.descriptionFAPESP: 2018/23639–0-
Formato: dc.formate275-e283-
Idioma: dc.languageen-
Relação: dc.relationDental Materials-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBiocompatible Materials-
Palavras-chave: dc.subjectCeramics-
Palavras-chave: dc.subjectHydroxyapatite-
Palavras-chave: dc.subjectTitanium dioxide-
Título: dc.titleTiO2 nanoparticles added to dense bovine hydroxyapatite bioceramics increase human osteoblast mineralization activity-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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