First report of Giardia duodenalis assemblage F in humans and dogs in southern Brazil

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Autor(es): dc.contributorUniversidade Estadual de Londrina (UEL)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorSilva, Ana Clécia dos Santos-
Autor(es): dc.creatorMartins, Felippe Danyel Cardoso-
Autor(es): dc.creatorLadeia, Winni Alves-
Autor(es): dc.creatorKakimori, Monica Tiemi Aline-
Autor(es): dc.creatorLucas, Juliana Izidoro-
Autor(es): dc.creatorSasse, João Pedro-
Autor(es): dc.creatorMartins, Thais Agostinho-
Autor(es): dc.creatorMacedo, Vilma de Fátima Rodrigues de-
Autor(es): dc.creatorMurad, Valter Abou-
Autor(es): dc.creatorde Barros, Luiz Daniel-
Autor(es): dc.creatorGarcia, João Luis-
Data de aceite: dc.date.accessioned2025-08-21T16:29:08Z-
Data de disponibilização: dc.date.available2025-08-21T16:29:08Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2022-10-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.cimid.2022.101878-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/245894-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/245894-
Descrição: dc.descriptionThe present study aimed to molecularly characterize Giardia duodenalis from stool samples of humans, dogs, and cats. Molecular analyses were performed on 59 samples that tested positive for G. duodenalis on coproparasitological examinations. After extraction, the samples were first tested by nested polymerase chain reaction (n-PCR) analysis of the SSU-rRNA gene, and for the samples that were positive, the β-giardin, TPI, and GDH genes were analyzed. The amplicons obtained in the n-PCR of the β-giardin gene were subjected to PCR-restriction length polymorphism (RFLP) analysis and subsequent digestion with the enzyme HaeIII to differentiate the assemblages. Seven (11.8 %), 34 (57.7 %), and 18 (30.5 %) out of 59 samples were from humans, dogs, and cats, respectively. Nested-PCR results showed that 49.2 % (29/59) of samples were positive for the SSU-rRNA gene, with 42.9 % (3/7) of humans, 55.9 % (19/34) of dogs, and 38.9 % (7/18) of catsve. Of the other genes analyzed, β-giardin was amplified most frequently, in 34.5 % (10/29) of samples, followed by GDH in 27.6 % (8/29) of samples, and TPI in 10.3 % (3/29) of samples. Only one sample from a dog showed the amplification of all genes. PCR-RFLP analysis showed assemblage F in a human, dog, and cat samples; and assemblage C and D in dog samples. This is the first description of assemblage F in humans from Brazil and the first description of assemblage F in dogs. Further studies are needed to verify the frequency with which these infections occur, and provide information that will contribute to the molecular epidemiological understanding of giardiasis.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionDepartment of Preventive Veterinary Medicine Laboratory of Animal Protozoology Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, Pr 445km 380, PR-
Descrição: dc.descriptionClinical Parasitology Laboratory of the University Hospital Ambulatory Hospital of Clínicas State University of Londrina -UEL, PR-
Descrição: dc.descriptionDepartment of Clinical Veterinary Faculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista, R. Dr. Valter Maurício Corrêa, s/n, SP-
Descrição: dc.descriptionDepartment of Clinical Veterinary Faculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista, R. Dr. Valter Maurício Corrêa, s/n, SP-
Descrição: dc.descriptionCNPq: 001-
Idioma: dc.languageen-
Relação: dc.relationComparative Immunology, Microbiology and Infectious Diseases-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectGiardiasis-
Palavras-chave: dc.subjectMolecular diagnosis-
Palavras-chave: dc.subjectOne health-
Palavras-chave: dc.subjectZoonosis-
Título: dc.titleFirst report of Giardia duodenalis assemblage F in humans and dogs in southern Brazil-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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