In Vitro Transdifferentiation Potential of Equine Mesenchymal Stem Cells into Schwann-Like Cells

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Autor(es): dc.contributorMed Sch Botucatu-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorFerreira, Lucas Vinicius de Oliveira-
Autor(es): dc.creatorKamura, Beatriz da Costa-
Autor(es): dc.creatorOliveira, Joao Pedro Marmo del-
Autor(es): dc.creatorChimenes, Natielly Dias-
Autor(es): dc.creatorCarvalho, Marcio de-
Autor(es): dc.creatorSantos, Leandro Alves dos-
Autor(es): dc.creatorDias-Melicio, Luciane Alarcao-
Autor(es): dc.creatorAmorim, Renee Laufer-
Autor(es): dc.creatorAmorim, Rogerio Martins-
Data de aceite: dc.date.accessioned2025-08-21T19:54:11Z-
Data de disponibilização: dc.date.available2025-08-21T19:54:11Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-06-07-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1089/scd.2022.0274-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/245660-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/245660-
Descrição: dc.descriptionSchwann cells (SCs) are essential for the regenerative processes of peripheral nerve injuries. However, their use in cell therapy is limited. In this context, several studies have demonstrated the ability of mesenchymal stem cells (MSCs) to transdifferentiate into Schwann-like cells (SLCs) using chemical protocols or co-culture with SCs. Here, we describe for the first time the in vitro transdifferentiation potential of MSCs derived from equine adipose tissue (AT) and equine bone marrow (BM) into SLCs using a practical method. In this study, the facial nerve of a horse was collected, cut into fragments, and incubated in cell culture medium for 48 h. This medium was used to transdifferentiate the MSCs into SLCs. Equine AT-MSCs and BM-MSCs were incubated with the induction medium for 5 days. After this period, the morphology, cell viability, metabolic activity, gene expression of glial markers glial fibrillary acidic protein (GFAP), myelin basic protein (MBP), p75 and S100 beta, nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and glial cell-derived neurotrophic factor (GDNF), and the protein expression of S100 and GFAP were evaluated in undifferentiated and differentiated cells. The MSCs from the two sources incubated with the induction medium exhibited similar morphology to the SCs and maintained cell viability and metabolic activity. There was a significant increase in the gene expression of BDNF, GDNF, GFAP, MBP, p75, and S100 beta in equine AT-MSCs and GDNF, GFAP, MBP, p75, and S100 beta in equine BM-MSCs post-differentiation. Immunofluorescence analysis revealed GFAP expression in undifferentiated and differentiated cells, with a significant increase in the integrated pixel density in differentiated cells and S100 was only expressed in differentiated cells from both sources. These findings indicate that equine AT-MSCs and BM-MSCs have great transdifferentiation potential into SLCs using this method, and they represent a promising strategy for cell-based therapy for peripheral nerve regeneration in horses.-
Descrição: dc.descriptionSchool of Veterinary Medicine and Animal Science-Sao Paulo State University (UNESP)-
Descrição: dc.descriptionCoordena��o de Aperfei�oamento de Pessoal de N�vel Superior (CAPES)-
Descrição: dc.descriptionMed Sch Botucatu, Sch Vet Med & Anim Sci, Dept Vet Clin, Botucatu, SP, Brazil-
Descrição: dc.descriptionMed Sch Botucatu, Sch Vet Med & Anim Sci, Translat Nucleus Regenerat Med NUTRAMERE, Botucatu, SP, Brazil-
Descrição: dc.descriptionMed Sch Botucatu, UNIPEX Expt Res Unit, Confocal Microscopy Lab, Botucatu, SP, Brazil-
Descrição: dc.descriptionMed Sch Botucatu, Dept Pathol, Botucatu, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ UNESP, Botucatu, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ UNESP, Sch Vet Med & Anim Sci, Med Sch Botucatu, Dept Vet Clin, Prof Doutor Walter Mauricio Correia St, BR-18618681 Botucatu, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ UNESP, Botucatu, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ UNESP, Sch Vet Med & Anim Sci, Med Sch Botucatu, Dept Vet Clin, Prof Doutor Walter Mauricio Correia St, BR-18618681 Botucatu, SP, Brazil-
Formato: dc.format11-
Idioma: dc.languageen-
Publicador: dc.publisherMary Ann Liebert, Inc-
Relação: dc.relationStem Cells And Development-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectfacial nerve-
Palavras-chave: dc.subjectnerve regeneration-
Palavras-chave: dc.subjectneurotrophic factors-
Palavras-chave: dc.subjectperipheral nerve injury-
Palavras-chave: dc.subjectSchwann-like cells-
Título: dc.titleIn Vitro Transdifferentiation Potential of Equine Mesenchymal Stem Cells into Schwann-Like Cells-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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