Bacterial RNA virus MS2 exposure increases the expression of cancer progression genes in the LNCaP prostate cancer cell line

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorEotvos Lorand Res Network-
Autor(es): dc.contributorUniv Warsaw-
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.creatorSanmukh, Swapnil Ganesh-
Autor(es): dc.creatorSantos, Nilton Jose dos-
Autor(es): dc.creatorBarquilha, Caroline Nascimento-
Autor(es): dc.creatorCarvalho, Marcio de-
Autor(es): dc.creatorReis, Patricia Pintor dos-
Autor(es): dc.creatorDelella, Flavia Karina-
Autor(es): dc.creatorCarvalho, Hernandes F. F.-
Autor(es): dc.creatorLatek, Dorota-
Autor(es): dc.creatorFeher, Tamas-
Autor(es): dc.creatorFelisbino, Sergio Luis-
Data de aceite: dc.date.accessioned2025-08-21T21:14:28Z-
Data de disponibilização: dc.date.available2025-08-21T21:14:28Z-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-07-29-
Data de envio: dc.date.issued2023-01-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3892/ol.2023.13672-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/245574-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/245574-
Descrição: dc.descriptionBacteriophages effectively counteract diverse bacterial infections, and their ability to treat most types of cancer has been explored using phage engineering or phage-virus hybrid platforms. In the present study, it was demonstrated that the bacteriophage MS2 can affect the expression of genes associated with the proliferation and survival of LNCaP prostate epithelial cells. LNCaP cells were exposed to bacteriophage MS2 at a concentration of 1x10(7) plaque forming units/ml for 24-48 h. After exposure, various cellular parameters, including cell viability, morphology, and changes in gene expression, were examined. MS2 affected cell viability adversely, reducing viability by 25% in the first 4 h of treatment; however, cell viability recovered within 24-48 h. Similarly, the AKT, androgen receptor, integrin alpha 5, integrin beta 1, MAPK1, MAPK3, STAT3, and peroxisome proliferator-activated receptor-gamma coactivator 1 alpha genes, which are involved in various normal cellular processes and tumor progression, were significantly upregulated, whereas the expression levels of HSP90, ITGB5, ITGB3, HSP27, ITGAV, and PI3K genes were unchanged. Therefore, based on viability and gene expression changes, bacteriophage MS2 severely impaired LNCaP cells by reducing anchorage-dependent survival and androgen signaling. A caveolin-mediated endocytosis mechanism for MS2-mediated signaling in prostate cancer cells was proposed based on reports involving bacteriophages T4, M13, and MS2, and their interactions with LNCaP and PC3 cell lines.-
Descrição: dc.descriptionSao Paulo State Univ, Inst Biosci Botucatu, Dept Struct & Funct Biol, Lab Extracellular Matrix Biol, BR-18618689 Botucatu, SP, Brazil-
Descrição: dc.descriptionEotvos Lorand Res Network, Biol Res Ctr, Synthet & Syst Biol Unit, H-6726 Szeged, Hungary-
Descrição: dc.descriptionUniv Warsaw, Fac Chem, PL-02093 Warsaw, Poland-
Descrição: dc.descriptionUniv Estadual Campinas, Inst Biol, Dept Struct & Funct Biol, BR-13083970 Campinas, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Fac Med, Dept Surg & Orthoped, BR-18618687 Botucatu, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Inst Biosci Botucatu, Dept Struct & Funct Biol, Lab Extracellular Matrix Biol, 250 Antonio Celso Wagner Zanin, BR-18618689 Botucatu, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Inst Biosci Botucatu, Dept Struct & Funct Biol, Lab Extracellular Matrix Biol, BR-18618689 Botucatu, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Fac Med, Dept Surg & Orthoped, BR-18618687 Botucatu, SP, Brazil-
Descrição: dc.descriptionSao Paulo State Univ, Inst Biosci Botucatu, Dept Struct & Funct Biol, Lab Extracellular Matrix Biol, 250 Antonio Celso Wagner Zanin, BR-18618689 Botucatu, SP, Brazil-
Formato: dc.format14-
Idioma: dc.languageen-
Publicador: dc.publisherSpandidos Publ Ltd-
Relação: dc.relationOncology Letters-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectandrogen receptor-
Palavras-chave: dc.subjectgene expression-
Palavras-chave: dc.subjectintegrin-
Palavras-chave: dc.subjectbacteriophage-
Palavras-chave: dc.subjectMS2-
Título: dc.titleBacterial RNA virus MS2 exposure increases the expression of cancer progression genes in the LNCaP prostate cancer cell line-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.