Cytotoxicity of resin-based light-cured liners

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorHebling, Josimeri-
Autor(es): dc.creatorLessa, Fernanda Campos Rosetti-
Autor(es): dc.creatorNogueira, Indri-
Autor(es): dc.creatorCarvalho, Ricardo Marins de-
Autor(es): dc.creatorSouza Costa, Carlos Alberto de-
Data de aceite: dc.date.accessioned2025-08-21T22:35:02Z-
Data de disponibilização: dc.date.available2025-08-21T22:35:02Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2013-09-30-
Data de envio: dc.date.issued2014-05-20-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2013-09-30-
Data de envio: dc.date.issued2014-05-20-
Data de envio: dc.date.issued2009-06-01-
Fonte completa do material: dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/19650592-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/243876-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/243876-
Descrição: dc.descriptionPurpose: To evaluate the cytotoxic effects of resin-based light-cured liners on culture of pulp cells. Methods: Discs measuring 4 mill in diameter and 2 mm thick were fabricated from TheraCal (TCMTA), Vitrebond (VIT), and Ultrablend Plus (UBP). These specimens were immersed in serum-free culture medium (DMEM) for 24 hours or 7 days to produce the extracts. After incubating the pulp cells for 72 hours, the extracts were applied on the cells and the cytotoxic effects were determined based on the cell metabolism (MTT), total protein expression and cell morphology (SEM). In the control group, fresh DMEM was used. Data from MTT analysis and protein expression were submitted to Kruskal-Wallis and Mann-Whitney tests at the preset level of significance of 5%. Results: When in contact with the 24-hour extract, TCMTA, VIT, and UBP decreased the cell metabolism by 31.5%, 73.5% and 71.0%, respectively. The total protein expressed by the cells in contact with VIT and UBP was lower than TCMTA and DMEM (Mann-Whitney, P< 0.05). When in contact with the 7-day extract, TCMTA, VIT, and UBP decreased the metabolic activity by 45.9%, 77.1% and 64.4%, respectively. All the liners expressed statistically lower amounts of proteins when compared to the control. A reduction in the number of cells was observed for all liners. The remaining cells from TCMTA group resembled those from the control group while for VIT and UBP the cells presented significant morphological alterations. (Ani J Dent 2009;22:137-142).-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionUniversity of São Paulo State UNESP, Rua Humaitá, 1680, CEP: 14.801-903, Araraquara, SP-
Descrição: dc.descriptionDepartment of Orthodontics and Pediatric Dentistry-
Descrição: dc.descriptionDepartment of Physiology and Pathology School of Dentistry University of São Paulo State, Araraquara, São Paulo-
Descrição: dc.descriptionDepartment of Prosthodontics School of Dentistry University of São Paulo, Bauru, SP-
Descrição: dc.descriptionUniv São Paulo State, UNESP, Dept Physiol & Pathol, Sch Dent, BR-14801903 Araraquara, SP, Brazil-
Descrição: dc.descriptionUniv São Paulo State, UNESP, Dept Orthodont & Pediat Dent, BR-14801903 Araraquara, SP, Brazil-
Descrição: dc.descriptionUniv São Paulo, Sch Dent, Dept Prosthodont, Bauru, SP, Brazil-
Descrição: dc.descriptionUniversity of São Paulo State UNESP, Rua Humaitá, 1680, CEP: 14.801-903, Araraquara, SP-
Descrição: dc.descriptionUniv São Paulo State, UNESP, Dept Physiol & Pathol, Sch Dent, BR-14801903 Araraquara, SP, Brazil-
Descrição: dc.descriptionUniv São Paulo State, UNESP, Dept Orthodont & Pediat Dent, BR-14801903 Araraquara, SP, Brazil-
Descrição: dc.descriptionCNPq: 476137/2006-3-
Descrição: dc.descriptionCNPq: 301029/2007-5-
Formato: dc.format137-142-
Idioma: dc.languageen-
Publicador: dc.publisherMosher & Linder, Inc-
Relação: dc.relationAmerican Journal of Dentistry-
Relação: dc.relation0.760-
Relação: dc.relation0,509-
Direitos: dc.rightsinfo:eu-repo/semantics/restrictedAccess-
???dc.source???: dc.sourceScopus-
???dc.source???: dc.sourceWeb of Science-
Título: dc.titleCytotoxicity of resin-based light-cured liners-
Tipo de arquivo: dc.typelivro digital-
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