Cytogenotoxicity assessment of 3-(3,4-dihydroxyphenyl)-7,8-dihydroxycoumarin on HepG2/C3A cells and leukocytes

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorFAMEMA-
Autor(es): dc.contributorUniversidad Estatal Amazonica-
Autor(es): dc.contributorUniversity of Santiago de Compostela-
Autor(es): dc.contributorUniversity of Porto-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorCastanha, Ana Paula Martins-
Autor(es): dc.creatorAlmeida-Terassi, Laíza Moura-
Autor(es): dc.creatorGuardado-Yordi, Estela-
Autor(es): dc.creatorMatos, Maria João-
Autor(es): dc.creatorMaistro, Edson Luis-
Data de aceite: dc.date.accessioned2025-08-21T22:00:45Z-
Data de disponibilização: dc.date.available2025-08-21T22:00:45Z-
Data de envio: dc.date.issued2023-03-02-
Data de envio: dc.date.issued2023-03-02-
Data de envio: dc.date.issued2021-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1002/jat.4384-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/242245-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/242245-
Descrição: dc.description3-(3,4-Dihydroxyphenyl)-7,8-dihydroxycoumarin is a newly synthesized coumarin derivative with a potent antioxidant effect. The aim of the present study is to investigate the safety of this compound, determining the in vitro cytotoxic and genotoxic in human peripheral blood mononuclear cells (PBMC) and in HepG2/C3A cells. Cell viability has been investigated by the trypan blue staining test and MTT assay and the genotoxicity by the comet assay and micronucleus test, using concentrations between 0.01 and 10 μg/ml. The compound proved to be noncytotoxic in both cell lines, at all tested concentrations, protecting the cells from the DNA damage. In addition, this molecule does not show clastogenic/aneugenic effects when performing the micronucleus test with cytokinesis blockade. Based on the obtained data, and the conditions of the experiments, we can conclude that the 3-(3,4-dihydroxyphenyl)-7,8-dihydroxycoumarin is a safe molecule up to a concentration of 10 μg/ml, which encourages further studies aiming to explore its potential as a drug candidate.-
Descrição: dc.descriptionMarilia Medical School FAMEMA-
Descrição: dc.descriptionUniversidad Estatal Amazonica-
Descrição: dc.descriptionDepartment of Organic Chemistry Faculty of Pharmacy University of Santiago de Compostela-
Descrição: dc.descriptionCIQUP/Department of Chemistry and Biochemistry Faculty of Sciences University of Porto-
Descrição: dc.descriptionFaculty of Philosophy and Sciences Speech and Hearing Therapy Department São Paulo State University—UNESP-
Descrição: dc.descriptionFaculty of Philosophy and Sciences Speech and Hearing Therapy Department São Paulo State University—UNESP-
Idioma: dc.languageen-
Relação: dc.relationJournal of Applied Toxicology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subject3-hydroxyphenylcoumarins-
Palavras-chave: dc.subjectcomet assay-
Palavras-chave: dc.subjectgenotoxic assessment-
Palavras-chave: dc.subjectmicronucleus test-
Palavras-chave: dc.subjectsynthetic coumarin-
Título: dc.titleCytogenotoxicity assessment of 3-(3,4-dihydroxyphenyl)-7,8-dihydroxycoumarin on HepG2/C3A cells and leukocytes-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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