Transcriptomic analysis of Malpighian tubules from the stingless bee Melipona scutellaris reveals thiamethoxam-induced damages

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.creatorMiotelo, Lucas-
Autor(es): dc.creatorFerro, Milene-
Autor(es): dc.creatorMaloni, Geovana-
Autor(es): dc.creatorOtero, Igor Vinicius Ramos-
Autor(es): dc.creatorNocelli, Roberta Cornélio Ferreira-
Autor(es): dc.creatorBacci, Mauricio-
Autor(es): dc.creatorMalaspina, Osmar-
Data de aceite: dc.date.accessioned2025-08-21T22:30:05Z-
Data de disponibilização: dc.date.available2025-08-21T22:30:05Z-
Data de envio: dc.date.issued2023-03-02-
Data de envio: dc.date.issued2023-03-02-
Data de envio: dc.date.issued2022-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.scitotenv.2022.158086-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/242160-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/242160-
Descrição: dc.descriptionThe concern about pesticide exposure to neotropical bees has been increasing in the last few years, and knowledge gaps have been identified. Although stingless bees, (e.g.: Melipona scutellaris), are more diverse than honeybees and they stand out in the pollination of several valuable economical crops, toxicity assessments with stingless bees are still scarce. Nowadays new approaches in ecotoxicological studies, such as omic analysis, were pointed out as a strategy to reveal mechanisms of how bees deal with these stressors. To date, no molecular techniques have been applied for the evaluation of target and/or non-target organs in stingless bees, such as the Malpighian tubules (Mt). Therefore, in the present study, we evaluated the differentially expressed genes (DEGs) in the Mt of M. scutellaris after one and eight days of exposure to LC50/100 (0.000543 ng a.i./μL) of thiamethoxam (TMX). Through functional annotation analysis of four transcriptome libraries, the time course line approach revealed 237 DEGs (nine clusters) associated with carbon/energy metabolism and cellular processes (lysosomes, autophagy, and glycan degradation). The expression profiles of Mt were altered by TMX in processes, such as detoxification, excretion, tissue regeneration, oxidative stress, apoptosis, and DNA repair. Transcriptome analysis showed that cell metabolism in Mt was mainly affected after 8 days of exposure. Nine genes were selected from different clusters and validated by RT-qPCR. According to our findings, TMX promotes several types of damage in Mt cells at the molecular level. Therefore, interference of different cellular processes directly affects the health of M. scutellaris by compromising the function of Mt.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionDepartment of General and Applied Biology Institute of Biosciences São Paulo State University (UNESP), SP-
Descrição: dc.descriptionCenter of Agrarian Sciences Federal University of São Carlos (UFSCar), Anhanguera Road Km 174-
Descrição: dc.descriptionDepartment of General and Applied Biology Institute of Biosciences São Paulo State University (UNESP), SP-
Descrição: dc.descriptionCNPq: 170714/2017-9-
Descrição: dc.descriptionFAPESP: 2017/21097-3-
Descrição: dc.descriptionFAPESP: 2020/03527-3-
Descrição: dc.descriptionFAPESP: 2021/01359-9-
Descrição: dc.descriptionCNPq: 400540/2018-5-
Descrição: dc.descriptionCAPES: 88887.310764/2018-00-
Descrição: dc.descriptionCAPES: 88887.572722/2020-00-
Idioma: dc.languageen-
Relação: dc.relationScience of the Total Environment-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectDe novo assembly-
Palavras-chave: dc.subjectDifferentially gene expressed-
Palavras-chave: dc.subjectExcretion organ-
Palavras-chave: dc.subjectNeonicotinoid-
Palavras-chave: dc.subjectSublethal effects-
Título: dc.titleTranscriptomic analysis of Malpighian tubules from the stingless bee Melipona scutellaris reveals thiamethoxam-induced damages-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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