High Frequency of Epstein-Barr Virus and Absence of Papillomavirus in Breast Cancer Patients from Brazilian Northeast

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorFederal University of Ceará-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorde Oliveira, Emanuele Silva-
Autor(es): dc.creatorFerreira, Márcia Valéria Pitombeira-
Autor(es): dc.creatorRahal, Paula-
Autor(es): dc.creatorBranco, Martan Barroso Castelo-
Autor(es): dc.creatorRabenhorst, Silvia Helena Barem-
Data de aceite: dc.date.accessioned2025-08-21T18:46:49Z-
Data de disponibilização: dc.date.available2025-08-21T18:46:49Z-
Data de envio: dc.date.issued2023-03-02-
Data de envio: dc.date.issued2023-03-02-
Data de envio: dc.date.issued2021-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.31557/APJCP.2022.23.7.2351-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/242105-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/242105-
Descrição: dc.descriptionObjective: This study aimed to determine the presence of Epstein-Barr Virus (EBV) and Human papillomavirus (HPV) in breast cancer with patients from Northeast of Brazil, considering the molecular subtypes and also taking in account the relation with TP53 immunoexpression. Methods: Seventy-five samples of invasive breast carcinoma with no special type were selected from pathology archives at Federal University of Ceará. EBV was detected by In situ hybridization (ISH) and immunohistochemistry (IHC) and HPV was detected by PCR. ISH was performed using EBER1 probe (Shibata et al., 1991; Bacchi et al., 1996) while IHC was performed on histological formalin-fixed paraffin-embedded tissue samples (Hsu et al., 1981). PCR methodology (Haws et al., 2004) was used to amplify the genetic material of human papillomavirus. The amplification products were electrophoretic analyzed on 1% agarose gel. The data analyses were carried out using the statistical software EPINFO® version 6.04d and SPSS version 17.0 (SPSS Inc., Chicago, IL). Statistically significant differences were evaluated by the chi-square test and Fisher’s exact test and correlations between groups were analyzed by Spearman’s and Pearson’s rank correlation coefficient. Results: 69.4% of the cases were EBNA1 positives by IHC. EBNA1 positive tumors had lower Ki-67 index (0-40%), while EBNA1 negative cases had relevant higher Ki-67 index (41-100%) (p = 0.06). EBV was present in all tumor grades, with a high frequency in grade I and III tumors comparing to EBNA1 negative cases. No HPV positive cases were observed. Conclusion: Regarding the results from this study, we support the hypothesis that EBV can be involved on breast tumorigenesis-
Descrição: dc.descriptionPathology and Forensic Medicine Department Federal University of Ceará, CE-
Descrição: dc.descriptionLaboratory of Genomic Studies Biology Department São Paulo State University (UNESP), SP-
Descrição: dc.descriptionLaboratory of Genomic Studies Biology Department São Paulo State University (UNESP), SP-
Formato: dc.format2351-2359-
Idioma: dc.languageen-
Relação: dc.relationAsian Pacific Journal of Cancer Prevention-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBiomarkers-
Palavras-chave: dc.subjectBreast-
Palavras-chave: dc.subjectEpidemiology-
Palavras-chave: dc.subjectMolecular oncology-
Título: dc.titleHigh Frequency of Epstein-Barr Virus and Absence of Papillomavirus in Breast Cancer Patients from Brazilian Northeast-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.