Inhibitory activity of S-PRG filler on collagen-bound MMPs and dentin matrix degradation

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade Estadual de Maringá (UEM)-
Autor(es): dc.creatorMendes Soares, Igor Paulino-
Autor(es): dc.creatorAnselmi, Caroline-
Autor(es): dc.creatorGuiné, Isabela-
Autor(es): dc.creatorFernandes, Lídia de Oliveira-
Autor(es): dc.creatorPires, Maria Luiza Barucci Araujo-
Autor(es): dc.creatorde Souza Costa, Carlos Alberto-
Autor(es): dc.creatorScheffel, Débora Lopes Salles-
Autor(es): dc.creatorHebling, Josimeri-
Data de aceite: dc.date.accessioned2025-08-21T16:22:27Z-
Data de disponibilização: dc.date.available2025-08-21T16:22:27Z-
Data de envio: dc.date.issued2023-03-02-
Data de envio: dc.date.issued2023-03-02-
Data de envio: dc.date.issued2022-09-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.jdent.2022.104237-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/242084-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/242084-
Descrição: dc.descriptionObjectives: To evaluate the inhibitory activity of an ion-releasing filler (S-PRG) eluate on dentin collagen-bound metalloproteinases (MMPs) and dentin matrix degradation. Methods: Dentin beams (5 × 2 × 0.5 mm) from human molars were completely demineralized to produce dentin matrix specimens. The dry mass was measured, and a colorimetric assay (Sensolyte) determined the initial total MMP activity to allocate the beams into four treatment groups (n = 10/group): 1) water for 1 min (negative control); 2) 2% chlorhexidine digluconate (CHX – inhibitor control) for 1 min; 3) S-PRG eluate for 1 min; 4) S-PRG eluate for 30 min. After the treatments, the total MMP activity was reassessed. The specimens were stored in simulated body fluid (SBF) at 37 °C for up to 21 days. The dry mass was reassessed weekly. On day 7, the dentin matrix degradation was analyzed for the presence of collagen fragments (CF; Sirius Red) and hydroxyproline (Hyp) in the SBF. Statistical analyses were performed with ANOVA/Tukey, paired t-tests, and RM-ANOVA/Sidak (α = 5%). Results: S-PRG eluate exposure for 1 and 30 min reduced (p < 0.0001) MMP activity. S-PRG exposure for 30 min presented MMP activity inhibition equivalent to CHX (p = 0.061). S-PRG and CHX decreased CF (p ≤ 0.007) and Hyp (p < 0.046) release. After 21 days of storage, S-PRG-treated beams, regardless of exposure time, presented a reduced (p ≤ 0.017) mass loss, intermediate between CHX and control. Conclusion: Treating demineralized dentin with S-PRG eluate for 1 or 30 min reduced matrix-bound MMP activity and dentin matrix degradation for up to 21 days. Clinical significance: S-PRG filler may hinder the progression of dentin carious/erosive lesions and enhance the stabilization of dentin bonding interfaces.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Genetics Morphology Orthodontics and Pediatric Dentistry School of Dentistry São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Restorative Dentistry School of Dentistry São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Physiology and Pathology School of Dentistry São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Dentistry Maringá State University (UEM), Paraná-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Genetics Morphology Orthodontics and Pediatric Dentistry School of Dentistry São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Restorative Dentistry School of Dentistry São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Physiology and Pathology School of Dentistry São Paulo State University (UNESP)-
Descrição: dc.descriptionCNPq: 138984/2020–4-
Descrição: dc.descriptionFAPESP: 2019/16473–1-
Descrição: dc.descriptionCNPq: 303391/2019–7-
Idioma: dc.languageen-
Relação: dc.relationJournal of Dentistry-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCollagen-
Palavras-chave: dc.subjectDentin-
Palavras-chave: dc.subjectHydroxyproline-
Palavras-chave: dc.subjectMMP-
Palavras-chave: dc.subjectPre-reacted glass filler-
Palavras-chave: dc.subjectProteolysis-
Título: dc.titleInhibitory activity of S-PRG filler on collagen-bound MMPs and dentin matrix degradation-
Tipo de arquivo: dc.typelivro digital-
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