Odd-chain fatty acids as an alternative method to predict ruminal microbial nitrogen flow of feedlot Nellore steers fed grain-based diets supplemented with different nitrogen sources

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorVirginia Polytechnic Institute and State University-
Autor(es): dc.contributorCIEI Nus-
Autor(es): dc.contributorUniversidade Federal de Viçosa (UFV)-
Autor(es): dc.creatorCampos, Letícia M.-
Autor(es): dc.creatorSouza, Vinícius C.-
Autor(es): dc.creatorGranja-Salcedo, Yury T.-
Autor(es): dc.creatorMessana, Juliana D.-
Autor(es): dc.creatorPrestegaard-Wilson, Jacquelyn M.-
Autor(es): dc.creatorGanga, Maria Júlia G.-
Autor(es): dc.creatorDias, Ana Veronica L.-
Autor(es): dc.creatorCosta, Vladimir E.-
Autor(es): dc.creatorBerchielli, Telma T.-
Data de aceite: dc.date.accessioned2025-08-21T22:41:55Z-
Data de disponibilização: dc.date.available2025-08-21T22:41:55Z-
Data de envio: dc.date.issued2023-03-02-
Data de envio: dc.date.issued2023-03-02-
Data de envio: dc.date.issued2022-05-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1093/jas/skac089-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/241980-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/241980-
Descrição: dc.descriptionThis study aimed to evaluate the use of total odd-chain fatty acids (OCFA) as a marker to estimate microbial nitrogen flow (MicN) and calculate the efficiency of microbial nitrogen synthesis (EMNS) in Nellore steers fed high-concentrate diets supplemented with different nitrogen supplements (NS). Ruminally and duodenally cannulated Nellore steers (n = 6; 354 ± 12 kg) were used in a 6 × 6 repeated switchback design balanced for residual effects. Treatments were arranged in a 3 × 3 factorial of three nitrogen (N) supplements (urea plus soybean meal; corn gluten meal; dried distillers' grains plus solubles) and three microbial markers (OCFA; double-labeled urea, 15N; microbial nucleic acid bases, MNAB). The total mixed ration was composed of fresh chopped sugarcane as the forage source in an 83:17 concentrate: forage ratio (dry matter basis). Linear regression was used to develop predictions of MicN from OCFA using 15N and MNAB as response variables. Microbial N flow was underestimated by the MNAB marker compared to 15N. Neither NS nor their respective interactions with the marker methods (MM) affected MicN or EMNS (P > 0.05). However, MicN was different for 15N and MNAB (P > 0.001 for both treatments). Marker methods affected EMNS in all energetic bases (total digestible carbohydrates P < 0.001; rumen-fermentable carbohydrates P < 0.001; organic matter truly degradable in the rumen P < 0.001). Equations that utilized OCFA as a regressor to predict MicN under different MM resulted in good fits of the data as observed by the coefficient of determination (R2; 15N = 0.78; MNAB = 0.69). Microbial N flow estimated from OCFA was overpredicted (15N by 7.46%; MNAB by 4.30%) compared with observed values. The OCFA model presented a small slope bias when methodological validation was applied (15N = 0.96%; MNAB = 3.90%), ensuring reliability of the proposed alternative method. Based on the conditions of this experiment, OCFA may be a suitable alternative to other methods that quantify MicN under different dietary conditions.-
Descrição: dc.descriptionDepartment of Animal Science São Paulo State University, SP-
Descrição: dc.descriptionDepartment of Dairy Science Virginia Polytechnic Institute and State University-
Descrição: dc.descriptionCorporación Colombiana de Investigación Agropecuaria - AGROSAVIA CIEI Nus, Antioquía-
Descrição: dc.descriptionDepartment of Physics and Biophysics São Paulo State University, SP-
Descrição: dc.descriptionINCT/CA-UFV-Department of Animal Science, MG-
Descrição: dc.descriptionDepartment of Animal Science São Paulo State University, SP-
Descrição: dc.descriptionDepartment of Physics and Biophysics São Paulo State University, SP-
Idioma: dc.languageen-
Relação: dc.relationJournal of Animal Science-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectisotopes-
Palavras-chave: dc.subjectmicrobial efficiency-
Palavras-chave: dc.subjectmicrobial markers-
Palavras-chave: dc.subjectmicrobial nucleic acid bases-
Título: dc.titleOdd-chain fatty acids as an alternative method to predict ruminal microbial nitrogen flow of feedlot Nellore steers fed grain-based diets supplemented with different nitrogen sources-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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