Modulating the lipid profile of blastocyst cell membrane with DPPC multilamellar vesicles

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorFederal Technological University of Paraná (UTFPR)-
Autor(es): dc.contributorNational Institute of Science and Technology in Bioanalytics (INCTBio)-
Autor(es): dc.creatorDe Rossi, Hugo-
Autor(es): dc.creatorBortoliero Costa, Camila-
Autor(es): dc.creatorRodrigues-Rossi, Luana Teixeira-
Autor(es): dc.creatorBarros Nunes, Giovana-
Autor(es): dc.creatorSpinosa Chéles, Dóris-
Autor(es): dc.creatorMaran Pereira, Isabella-
Autor(es): dc.creatorRocha, Daniele F. O.-
Autor(es): dc.creatorFeitosa, Eloi-
Autor(es): dc.creatorColnaghi Simionato, Ana Valéria-
Autor(es): dc.creatorZoccal Mingoti, Gisele-
Autor(es): dc.creatorBenites Aoki, Pedro Henrique-
Autor(es): dc.creatorGouveia Nogueira, Marcelo Fábio-
Data de aceite: dc.date.accessioned2025-08-21T18:17:48Z-
Data de disponibilização: dc.date.available2025-08-21T18:17:48Z-
Data de envio: dc.date.issued2023-03-02-
Data de envio: dc.date.issued2023-03-02-
Data de envio: dc.date.issued2021-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1080/21691401.2022.2088545-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/241943-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/241943-
Descrição: dc.descriptionThe aim of this study was to evaluate the effect of multilamellar vesicles (MLVs) of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) in co-culture with in vitro-produced bovine embryos (IVPEs). The stability of five concentrations of MLVs (1.0, 1.25, 1.5, 1.75, and 2.0 mM) produced using ultrapure water or embryonic culture medium with 24 or 48 h of incubation at 38.5 °C with 5% CO2 was assessed. In addition, the toxicity of MLVs and their modulation of the lipid profile of the plasma membrane of IVPEs were evaluated after 48 h of co-culture. Both media allowed the production of MLVs. Incubation (24 and 48 h) did not impair the MLV structure but affected the average diameter. The rate of blastocyst production was not reduced, demonstrating the nontoxicity of the MLVs even at 2.0 mmol/L. The lipid profile of the embryos was different depending on the MLV concentration. In comparison with control embryos, embryos cultured with MLVs at 2.0 mmol/L had a higher relative abundance of six lipid ions (m/z 720.6, 754.9, 759.0, 779.1, 781.2, and 797.3). This study sheds light on a new culture system in which the MLV concentration could change the lipid profile of the embryonic cell membrane in a dose-dependent manner.-
Descrição: dc.descriptionDepartment of Biological Sciences School of Sciences and Languages São Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionGraduate Program in Pharmacology and Biotechnology Institute of Biosciences UNESP, São Paulo-
Descrição: dc.descriptionSchool of Veterinary Medicine Laboratory of Reproductive Physiology UNESP, São Paulo-
Descrição: dc.descriptionChemistry Institute University of Campinas and Pontifical Catholic University of Campinas, São Paulo-
Descrição: dc.descriptionAcademic Department of Chemistry and Biology Federal Technological University of Paraná (UTFPR), Paraná-
Descrição: dc.descriptionLaboratory of Analysis of Biomolecules Tiselius University of Campinas (UNICAMP), São Paulo-
Descrição: dc.descriptionNational Institute of Science and Technology in Bioanalytics (INCTBio), São Paulo-
Descrição: dc.descriptionDepartment of Biotechnology School of Sciences and Languages UNESP, São Paulo-
Descrição: dc.descriptionDepartment of Biological Sciences School of Sciences and Languages São Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionGraduate Program in Pharmacology and Biotechnology Institute of Biosciences UNESP, São Paulo-
Descrição: dc.descriptionSchool of Veterinary Medicine Laboratory of Reproductive Physiology UNESP, São Paulo-
Descrição: dc.descriptionDepartment of Biotechnology School of Sciences and Languages UNESP, São Paulo-
Formato: dc.format158-167-
Idioma: dc.languageen-
Relação: dc.relationArtificial Cells, Nanomedicine and Biotechnology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subject1,2-dipalmitoyl-sn-glycero-3-phosphocholine-
Palavras-chave: dc.subjectbovine embryo-
Palavras-chave: dc.subjectin vitro culture-
Palavras-chave: dc.subjectLipid vesicle-
Palavras-chave: dc.subjectMALDI-MS-
Palavras-chave: dc.subjectplasma membrane lipid-
Título: dc.titleModulating the lipid profile of blastocyst cell membrane with DPPC multilamellar vesicles-
Tipo de arquivo: dc.typelivro digital-
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