Inactivation of SARS-CoV-2 by a chitosan/α-Ag2WO4 composite generated by femtosecond laser irradiation

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Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.contributorUniversity Jaume I (UJI)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorOswaldo Cruz Institute-
Autor(es): dc.contributorUniversitat Jaume I-
Autor(es): dc.contributorUniversity of Wuppertal-
Autor(es): dc.creatorPereira, Paula Fabiana Santos-
Autor(es): dc.creatorde Paula e Silva, Ana Carolina Alves-
Autor(es): dc.creatorda Silva Pimentel, Bruna Natália Alves-
Autor(es): dc.creatorPinatti, Ivo Mateus-
Autor(es): dc.creatorSimões, Alexandre Zirpoli-
Autor(es): dc.creatorVergani, Carlos Eduardo-
Autor(es): dc.creatorBarreto-Vieira, Débora Ferreira-
Autor(es): dc.creatorda Silva, Marcos Alexandre Nunes-
Autor(es): dc.creatorMiranda, Milene Dias-
Autor(es): dc.creatorMonteiro, Maria Eduarda Santos-
Autor(es): dc.creatorTucci, Amanda-
Autor(es): dc.creatorDoñate-Buendía, Carlos-
Autor(es): dc.creatorMínguez-Vega, Gladys-
Autor(es): dc.creatorAndrés, Juan-
Autor(es): dc.creatorLongo, Elson-
Data de aceite: dc.date.accessioned2025-08-21T15:46:56Z-
Data de disponibilização: dc.date.available2025-08-21T15:46:56Z-
Data de envio: dc.date.issued2023-03-02-
Data de envio: dc.date.issued2023-03-02-
Data de envio: dc.date.issued2022-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1038/s41598-022-11902-5-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/241847-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/241847-
Descrição: dc.descriptionIn the current COVID-19 pandemic, the next generation of innovative materials with enhanced anti-SARS-CoV-2 activity is urgently needed to prevent the spread of this virus within the community. Herein, we report the synthesis of chitosan/α-Ag2WO4 composites synthetized by femtosecond laser irradiation. The antimicrobial activity against Escherichia coli, Methicilin-susceptible Staphylococcus aureus (MSSA), and Candida albicans was determined by estimating the minimum inhibitory concentration (MIC) and minimal bactericidal/fungicidal concentration (MBC/MFC). To assess the biocompatibility of chitosan/α-Ag2WO4 composites in a range involving MIC and MBC/MFC on keratinocytes cells (NOK-si), an alamarBlue™ assay and an MTT assay were carried out. The SARS-CoV-2 virucidal effects was analyzed in Vero E6 cells through viral titer quantified in cell culture supernatant by PFU/mL assay. Our results showed a very similar antimicrobial activity of chitosan/α-Ag2WO4 3.3 and 6.6, with the last one demonstrating a slightly better action against MSSA. The chitosan/α-Ag2WO4 9.9 showed a wide range of antimicrobial activity (0.49–31.25 µg/mL). The cytotoxicity outcomes by alamarBlue™ revealed that the concentrations of interest (MIC and MBC/MFC) were considered non-cytotoxic to all composites after 72 h of exposure. The Chitosan/α-Ag2WO4 (CS6.6/α-Ag2WO4) composite reduced the SARS-CoV-2 viral titer quantification up to 80% of the controls. Then, our results suggest that these composites are highly efficient materials to kill bacteria (Escherichia coli, Methicillin-susceptible Staphylococcus aureus, and the yeast strain Candida albicans), in addition to inactivating SARS-CoV-2 by contact, through ROS production.-
Descrição: dc.descriptionCDMF LIEC Department of Chemistry Federal University of São Carlos (UFSCar), P.O. Box 676, SP-
Descrição: dc.descriptionDepartment of Physical and Analytical Chemistry University Jaume I (UJI)-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP), 1680 Humaitá Street, SP-
Descrição: dc.descriptionFaculty of Engineering of Guaratinguetá São Paulo State University (UNESP), SP-
Descrição: dc.descriptionLaboratory of Viral Morphology and Morphogenesis Oswaldo Cruz Institute, Fiocruz, Avenida Brasil-
Descrição: dc.descriptionLaboratory of Respiratory Viruses and Measles Oswaldo Cruz Institute, Fiocruz, Avenida Brasil-
Descrição: dc.descriptionGROC UJI Institute of New Imaging Technologies Universitat Jaume I, Avda. Sos Baynat sn-
Descrição: dc.descriptionMaterials Science and Additive Manufacturing University of Wuppertal, Gaußstr. 20-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP), 1680 Humaitá Street, SP-
Descrição: dc.descriptionFaculty of Engineering of Guaratinguetá São Paulo State University (UNESP), SP-
Idioma: dc.languageen-
Relação: dc.relationScientific Reports-
???dc.source???: dc.sourceScopus-
Título: dc.titleInactivation of SARS-CoV-2 by a chitosan/α-Ag2WO4 composite generated by femtosecond laser irradiation-
Tipo de arquivo: dc.typelivro digital-
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