Evaluation of a serum-free culture medium for the enhanced vitrification cryosurvival of bovine in vitro-derived embryos

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorFederal University of Pampa-
Autor(es): dc.contributorABS Pecplan-
Autor(es): dc.contributorFederal University of ABC-
Autor(es): dc.contributorSupera Innovation and Technology Park-
Autor(es): dc.contributorUFG / UFCAT-
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorMackenzie Presbyterian University-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.creatorde Camargo, Janine-
Autor(es): dc.creatorRodrigues, Rafaela-
Autor(es): dc.creatorValente, Roniele Santana-
Autor(es): dc.creatorMuller, Diego Borba-
Autor(es): dc.creatorVireque, Alessandra Aparecida-
Autor(es): dc.creatorBelaz, Katia Roberta Anacleto-
Autor(es): dc.creatorBohrer, Rodrigo Camponogara-
Autor(es): dc.creatorBasso, Andrea Cristina-
Autor(es): dc.creatorEberlin, Marcos Nogueira-
Autor(es): dc.creatorFontes, Patricia Kubo-
Autor(es): dc.creatorNogueira, Marcelo Fábio Gouveia-
Autor(es): dc.creatorSudano, Mateus José-
Data de aceite: dc.date.accessioned2025-08-21T19:44:27Z-
Data de disponibilização: dc.date.available2025-08-21T19:44:27Z-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2022-06-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.livsci.2022.104922-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/241746-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/241746-
Descrição: dc.descriptionThis is a feasibility study aimed to determine the effectiveness of a culture media designed to enhance embryonic cryopreservation based on laboratory-accessed quality phenotypes of in vitro produced bovine embryos. Recovered bovine oocytes were matured and fertilized in vitro and the presumptive zygotes that were obtained were randomly distributed into three culture media: (i) Control (C): modified synthetic oviduct fluid (mSOF) culture medium supplemented with 5 mg/mL BSA and 2.5% fetal calf serum; (ii) Medium A (MA): serum-free altered mSOF medium A; and iii) Medium B (MB): serum-free altered mSOF medium B. Embryos cultured in MA and MB presented a better viability after cryopreservation (P < 0.05) without affecting the production rates of blastocysts (P > 0.05, MB only) when compared to C. The MB-derived blastocysts had the highest (P < 0.05) hatching rate (26.1%, 53/203) and higher (P < 0.05) transferable embryos rate (92.1%, 187/203) compared to control-derived blastocysts (85.8%, 175/204). The total cell number in the embryos after vitrification/warming process was greater (P < 0.05) in the MA (144.9 ± 5.9) and MB (151.3 ± 7.1) compared to that in the control (121.4 ± 6.1). The number of large and giant cytoplasmic lipid droplets was reduced (P < 0.05) in MB-derived embryos compared to control-derived embryos. Additionally, the lipid profiles of MB-derived blastocysts were closely related to those observed in the in vivo-derived blastocysts. We observed different transcriptional profiles abundance of in vitro produced bovine embryos derived from control, MA and MB groups. Therefore, the novel MB culture medium increased embryonic cryosurvival and favored the production of bovine embryos in vitro with improved cellular and molecular laboratory-accessed quality phenotypes.-
Descrição: dc.descriptionSchool of Veterinary Medicine Federal University of Pampa, RS-
Descrição: dc.descriptionIn Vitro Brasil ABS Pecplan, SP-
Descrição: dc.descriptionCenter for Natural and Human Sciences Federal University of ABC, SP-
Descrição: dc.descriptionInvitra – Assisted Reproductive Technologies Ltd. Supera Innovation and Technology Park, São Paulo-
Descrição: dc.descriptionInstitute of Chemistry Federal University of Catalão UFG / UFCAT, GO-
Descrição: dc.descriptionThoMSon Mass Spectrometry Laboratory Institute of Chemistry University of Campinas, SP-
Descrição: dc.descriptionSchool of Engineering Mackenzie Presbyterian University, SP-
Descrição: dc.descriptionMulti-user Laboratory of Phytomedicines Pharmacology and Biotechnology (PhitoPharmaTec) Department of Pharmacology São Paulo State University Institute of Biosciences-
Descrição: dc.descriptionDepartment of Biological Sciences School of Sciences and Languages São Paulo State University-
Descrição: dc.descriptionDepartment of Genetics and Evolution Federal University of São Carlos, São Paulo-
Descrição: dc.descriptionMulti-user Laboratory of Phytomedicines Pharmacology and Biotechnology (PhitoPharmaTec) Department of Pharmacology São Paulo State University Institute of Biosciences-
Descrição: dc.descriptionDepartment of Biological Sciences School of Sciences and Languages São Paulo State University-
Idioma: dc.languageen-
Relação: dc.relationLivestock Science-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBovine embryo-
Palavras-chave: dc.subjectCryosurvival-
Palavras-chave: dc.subjectLipids-
Palavras-chave: dc.subjectVitrification-
Título: dc.titleEvaluation of a serum-free culture medium for the enhanced vitrification cryosurvival of bovine in vitro-derived embryos-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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