Modular Label-Free Electrochemical Biosensor Loading Nature-Inspired Peptide toward the Widespread Use of COVID-19 Antibody Tests

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Autor(es): dc.contributorBrazilian Center for Research in Energy and Materials-
Autor(es): dc.contributorFederal University of ABC-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorHarvard University-
Autor(es): dc.creatorCastro, Ana C. H.-
Autor(es): dc.creatorBezerra, Ítalo R. S.-
Autor(es): dc.creatorPascon, Aline M.-
Autor(es): dc.creatorDa Silva, Gabriela H.-
Autor(es): dc.creatorPhilot, Eric A.-
Autor(es): dc.creatorDe Oliveira, Vivian L.-
Autor(es): dc.creatorMancini, Rodrigo S. N.-
Autor(es): dc.creatorSchleder, Gabriel R.-
Autor(es): dc.creatorCastro, Carlos E.-
Autor(es): dc.creatorDe Carvalho, Luciani R. S.-
Autor(es): dc.creatorFernandes, Bianca H. V.-
Autor(es): dc.creatorCilli, Eduardo M.-
Autor(es): dc.creatorSanches, Paulo R. S.-
Autor(es): dc.creatorSanthiago, Murilo-
Autor(es): dc.creatorCharlie-Silva, Ives-
Autor(es): dc.creatorMartinez, Diego S. T.-
Autor(es): dc.creatorScott, Ana L.-
Autor(es): dc.creatorAlves, Wendel A.-
Autor(es): dc.creatorLima, Renato S.-
Data de aceite: dc.date.accessioned2025-08-21T19:48:49Z-
Data de disponibilização: dc.date.available2025-08-21T19:48:49Z-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2021-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1021/acsnano.2c04364-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/241701-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/241701-
Descrição: dc.descriptionLimitations of the recognition elements in terms of synthesis, cost, availability, and stability have impaired the translation of biosensors into practical use. Inspired by nature to mimic the molecular recognition of the anti-SARS-CoV-2 S protein antibody (AbS) by the S protein binding site, we synthesized the peptide sequence of Asn-Asn-Ala-Thr-Asn-COOH (abbreviated as PEP2003) to create COVID-19 screening label-free (LF) biosensors based on a carbon electrode, gold nanoparticles (AuNPs), and electrochemical impedance spectroscopy. The PEP2003 is easily obtained by chemical synthesis, and it can be adsorbed on electrodes while maintaining its ability for AbS recognition, further leading to a sensitivity 3.4-fold higher than the full-length S protein, which is in agreement with the increase in the target-to-receptor size ratio. Peptide-loaded LF devices based on noncovalent immobilization were developed by affording fast and simple analyses, along with a modular functionalization. From studies by molecular docking, the peptide-AbS binding was found to be driven by hydrogen bonds and hydrophobic interactions. Moreover, the peptide is not amenable to denaturation, thus addressing the trade-off between scalability, cost, and robustness. The biosensor preserves 95.1% of the initial signal for 20 days when stored dry at 4 °C. With the aid of two simple equations fitted by machine learning (ML), the method was able to make the COVID-19 screening of 39 biological samples into healthy and infected groups with 100.0% accuracy. By taking advantage of peptide-related merits combined with advances in surface chemistry and ML-aided accuracy, this platform is promising to bring COVID-19 biosensors into mainstream use toward straightforward, fast, and accurate analyses at the point of care, with social and economic impacts being achieved.-
Descrição: dc.descriptionBrazilian Nanotechnology National Laboratory Brazilian Center for Research in Energy and Materials, Campinas-
Descrição: dc.descriptionCenter for Natural and Human Sciences Federal University of ABC, Santo André-
Descrição: dc.descriptionInstitute of Biomedical Sciences University of São Paulo São Paulo-
Descrição: dc.descriptionInstitute of Chemistry University of Campinas, Campinas-
Descrição: dc.descriptionSão Carlos Institute of Chemistry University of São Paulo, São Carlos-
Descrição: dc.descriptionLaboratory of Immunology Heart Institute University of São Paulo São Paulo-
Descrição: dc.descriptionCenter for Mathematics Computing and Cognition Federal University of ABC, Santo André-
Descrição: dc.descriptionMedical School University of Sao Paulo São Paulo-
Descrição: dc.descriptionInstitute of Chemistry São Paulo State University, Araraquara-
Descrição: dc.descriptionJohn A. Paulson School of Engineering and Applied Sciences Harvard University-
Descrição: dc.descriptionInstitute of Chemistry São Paulo State University, Araraquara-
Idioma: dc.languageen-
Relação: dc.relationACS Nano-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectelectrochemical impedance spectroscopy-
Palavras-chave: dc.subjectgold nanoparticle-
Palavras-chave: dc.subjectmachine learning-
Palavras-chave: dc.subjectnoncovalent immobilization-
Palavras-chave: dc.subjectSARS-CoV-2-
Título: dc.titleModular Label-Free Electrochemical Biosensor Loading Nature-Inspired Peptide toward the Widespread Use of COVID-19 Antibody Tests-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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