Dissecting and Reconstructing Matrix in Malignant Mesothelioma Through Histocell-Histochemistry Gradients for Clinical Applications

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Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorBalancin, Marcelo Luiz-
Autor(es): dc.creatorBaldavira, Camila Machado-
Autor(es): dc.creatorPrieto, Tabatha Gutierrez-
Autor(es): dc.creatorMachado-Rugolo, Juliana-
Autor(es): dc.creatorFarhat, Cecília-
Autor(es): dc.creatorAssato, Aline Kawassaki-
Autor(es): dc.creatorVelosa, Ana Paula Pereira-
Autor(es): dc.creatorTeodoro, Walcy Rosolia-
Autor(es): dc.creatorAb'Saber, Alexandre Muxfeldt-
Autor(es): dc.creatorTakagaki, Teresa Yae-
Autor(es): dc.creatorCapelozzi, Vera Luiza-
Data de aceite: dc.date.accessioned2025-08-21T19:01:27Z-
Data de disponibilização: dc.date.available2025-08-21T19:01:27Z-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2022-04-13-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3389/fmed.2022.871202-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/241644-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/241644-
Descrição: dc.descriptionBackground: Malignant pleural mesotheliomas (MM) are known for their heterogenous histology and clinical behavior. MM histology reveals three major tumor cell populations: epithelioid, sarcomatoid, and biphasic. Using a dissecting approach, we showed that histochemical gradients help us better understand tumor heterogeneity and reconsider its histologic classifications. We also showed that this method to characterize MM tumor cell populations provides a better understanding of the underlying mechanisms for invasion and disease progression. Methods: In a cohort of 87 patients with surgically excised MM, we used hematoxylin and eosin to characterize tumor cell populations and Movat's pentachrome staining to dissect the ECM matrisome. Next, we developed a computerized semi-assisted protocol to quantify and reconstruct the ECM in 3D and examined the clinical association between the matricellular factors and patient outcome. Results: Epithelioid cells had a higher matrix composition of elastin and fibrin, whereas, in the sarcomatoid type, hyaluronic acid and total collagen were most prevalent. The 3D reconstruction exposed the collagen I and III that form channels surrounding the neoplastic cell blocks. The estimated volume of the two collagen fractions was 14% of the total volume, consistent with the median estimated area of total collagen (12.05 mm2) for epithelioid MM. Conclusion: Differential patterns in matricellular phenotypes in MM could be used in translational studies to improve patient outcome. More importantly, our data raise the possibility that cancer cells can use the matrisome for disease expansion and could be effectively targeted by anti-collagen, anti-elastin, and/or anti-hyaluronic acid therapies.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionLaboratory of Genomics and Histomorphometry Department of Pathology University of São Paulo Medical School (USP)-
Descrição: dc.descriptionHealth Technology Assessment Center (NATS) Clinical Hospital (HCFMB) Medical School of São Paulo State University (UNESP)-
Descrição: dc.descriptionRheumatology Division of the Hospital das Clinicas da Faculdade de Medicina da Universidade de São Paulo FMUSP-
Descrição: dc.descriptionDivision of Pneumology Instituto do Coração (Incor) University of São Paulo Medical School (USP)-
Descrição: dc.descriptionHealth Technology Assessment Center (NATS) Clinical Hospital (HCFMB) Medical School of São Paulo State University (UNESP)-
Idioma: dc.languageen-
Relação: dc.relationFrontiers in Medicine-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectimmunohistochemistry-
Palavras-chave: dc.subjectmalignant mesothelioma-
Palavras-chave: dc.subjectMovat's pentachrome stain-
Palavras-chave: dc.subjectPicrosirius-
Palavras-chave: dc.subjectprognosis-
Título: dc.titleDissecting and Reconstructing Matrix in Malignant Mesothelioma Through Histocell-Histochemistry Gradients for Clinical Applications-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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