Frequency, spatial distribution, and genetic diversity of Blastocystis among referred individuals to a clinical laboratory: First report of subtype 9 in Brazil

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorSagrado Coração University (USC)-
Autor(es): dc.contributorIntegrated Faculties of Bauru (FIB)-
Autor(es): dc.creatorBertozzo, Thainá Valente-
Autor(es): dc.creatorDavid, Érica Boarato-
Autor(es): dc.creatorOliveira-Arbex, Ana Paula-
Autor(es): dc.creatorVictória, Cassiano-
Autor(es): dc.creatorGuimarães, Semíramis-
Data de aceite: dc.date.accessioned2025-08-21T20:53:37Z-
Data de disponibilização: dc.date.available2025-08-21T20:53:37Z-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2022-10-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.actatropica.2022.106608-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/241389-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/241389-
Descrição: dc.descriptionThe enteric protist Blastocystis has a worldwide distribution, however its prevalence in the human population is still underestimated, especially in developing countries where proper diagnosis is not performed in the routine of clinical laboratories. In this study, we aimed to assess the frequency, genetic diversity, and spatial distribution of Blastocystis isolates detected in fecal samples referred to a clinical laboratory for routine examination in inner São Paulo State, Brazil. A total of 348 leftover stool samples available for disposal from female and male individuals with age ranging from 3 months to 88 years were analyzed by both microscopic examination and PCR/sequencing of the SSU rRNA gene. The overall frequency of Blastocystis sp. was 31% (108/348), including 20.1% (70/348) and 31% (108/348) by microscopic examination and PCR/sequencing, respectively. Significant association was found only between Blastocystis infection and age, since the highest rate of positive samples was detected among 5–9 years old individuals (p < 0.0001). In addition, spatial distribution revealed a wide distribution of the positive samples, however they were densely concentrated in more populated areas. Seven subtypes were identified, namely ST1 (40.7%), ST2 (9.2%), ST3 (45.3%), ST4 (0.9%), ST6 (1.8%), ST7 (0.9%) and ST9 (0.9%). The intra-subtype analysis revealed a total of 25 different alleles previously reported. Here, the findings lead us to highlight the following aspects: (1) the identification of a ST9 isolate is a relevant finding since it is considered a very rare subtype in human infections as well as this is the first report in Brazil; (2) the high frequency of Blastocystis in fecal samples submitted for examination in a clinical laboratory points to the need to consider its search in routine parasitological examinations, (3) the spatial distribution of Blastocystis infection was not homogeneous but concentrated in more populated areas where the access for population to diagnostic services in healthcare is likely to be easier and, (4) the genetic variability of Blastocystis isolates suggests exposure of inhabitants living in inner municipalities to different sources of contamination involving anthroponotic and zoonotic transmission pathways.-
Descrição: dc.descriptionTropical Diseases Posgraduate Program Medical School São Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Health Sciences Sagrado Coração University (USC), SP-
Descrição: dc.descriptionIntegrated Faculties of Bauru (FIB), SP-
Descrição: dc.descriptionFaculty of Veterinary Medicine and Animal Science Department of Animal Production and Preventive Veterinary Medicine São Paulo State University (UNESP), SP-
Descrição: dc.descriptionInstitute of Bioscience Division of Parasitology São Paulo State University (UNESP), Campus de Botucatu, PO Box: 510-
Descrição: dc.descriptionTropical Diseases Posgraduate Program Medical School São Paulo State University (UNESP), SP-
Descrição: dc.descriptionFaculty of Veterinary Medicine and Animal Science Department of Animal Production and Preventive Veterinary Medicine São Paulo State University (UNESP), SP-
Descrição: dc.descriptionInstitute of Bioscience Division of Parasitology São Paulo State University (UNESP), Campus de Botucatu, PO Box: 510-
Idioma: dc.languageen-
Relação: dc.relationActa Tropica-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBlastocystis sp-
Palavras-chave: dc.subjectClinical laboratory-
Palavras-chave: dc.subjectFrequency-
Palavras-chave: dc.subjectSpatial analysis-
Palavras-chave: dc.subjectSubtypes-
Título: dc.titleFrequency, spatial distribution, and genetic diversity of Blastocystis among referred individuals to a clinical laboratory: First report of subtype 9 in Brazil-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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