Rapamycin did not prevent the excessive exercise-induced hepatic fat accumulation

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Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorUniversity of Waterloo-
Autor(es): dc.creatorPinto, Ana P.-
Autor(es): dc.creatorda Rocha, Alisson L.-
Autor(es): dc.creatorTeixeira, Giovana R.-
Autor(es): dc.creatorRovina, Rafael L.-
Autor(es): dc.creatorVeras, Allice S.C.-
Autor(es): dc.creatorFrantz, Fabiani-
Autor(es): dc.creatorPauli, José R.-
Autor(es): dc.creatorde Moura, Leandro P.-
Autor(es): dc.creatorCintra, Dennys E.-
Autor(es): dc.creatorRopelle, Eduardo R.-
Autor(es): dc.creatorQuadrilatero, Joe-
Autor(es): dc.creatorda Silva, Adelino S.R.-
Data de aceite: dc.date.accessioned2025-08-21T16:05:37Z-
Data de disponibilização: dc.date.available2025-08-21T16:05:37Z-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2022-10-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.lfs.2022.120800-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/241351-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/241351-
Descrição: dc.descriptionAims: The excessive eccentric exercise led to hepatic fat accumulation, which occurred concomitantly with elevation in the mammalian target of the rapamycin complex 1 (mTORC1) and insulin signaling pathways. Since mTORC1 and insulin can inhibit the autophagy pathway and explain the liver lipid content elevation, the main objective of the present investigation was to verify the responses of genes and proteins related to the autophagy and lipogenesis pathways in the hepatic tissue of mice submitted to the excessive downhill running protocol with and without rapamycin administration, a drug able to inhibit the mTORC1 pathway. Main methods: C57BL/6J mice were divided into four experimental groups: Control (CT; sedentary), Excessive exercise in downhill running (EE), Excessive exercise in downhill running with chronic administration of rapamycin (EE/Rapa), and Endurance exercise (END). At the end of the protocols, the blood and liver were collected for serum analysis, histology, immunohistochemistry, hepatic fat content, reverse transcription-quantitative polymerase chain reaction, and immunoblotting. Key findings: 1) higher levels of glucose, insulin, HOMA-IR, cortisol, ALT, and cholesterol, but lower levels of T4 for the EE/Rapa group; 2) hepatic fat accumulation for the EE and EE/Rapa groups; 3) upregulation of LC3 immunoexpression and downregulation of autophagic flux index for the EE and EE/Rapa groups; 4) reduction of P70S6K phosphorylation and SQSTM1 and increase of FOXO1A phosphorylation for the EE/Rapa group. Significance: The excessive exercise in downhill running with or without rapamycin led to increased liver fat content. Although rapamycin was effective in inhibiting mTORC1, the autophagy flux was not upregulated.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionPostgraduate Program in Rehabilitation and Functional Performance Ribeirão Preto Medical School University of São Paulo (USP), São Paulo-
Descrição: dc.descriptionMulticentric Program of Postgraduate in Physiological Sciences São Paulo State University (UNESP) School of Dentistry of Araçatuba, São Paulo-
Descrição: dc.descriptionDepartment of Physical Education State University of São Paulo (UNESP), São Paulo-
Descrição: dc.descriptionSchool of Physical Education and Sport of Ribeirão Preto University of São Paulo (USP), São Paulo-
Descrição: dc.descriptionFaculty of Pharmaceutical Sciences of Ribeirão Preto Department of Clinical Toxicological and Bromatological Analysis University of São Paulo (USP), São Paulo-
Descrição: dc.descriptionLaboratory of Molecular Biology of Exercise (LaBMEx) School of Applied Sciences University of Campinas (UNICAMP), São Paulo-
Descrição: dc.descriptionDepartment of Kinesiology and Health Sciences University of Waterloo-
Descrição: dc.descriptionMulticentric Program of Postgraduate in Physiological Sciences São Paulo State University (UNESP) School of Dentistry of Araçatuba, São Paulo-
Descrição: dc.descriptionDepartment of Physical Education State University of São Paulo (UNESP), São Paulo-
Descrição: dc.descriptionCAPES: 001-
Descrição: dc.descriptionFAPESP: 2017/09038-1-
Descrição: dc.descriptionFAPESP: 2017/12765-2-
Descrição: dc.descriptionFAPESP: 2017/19869-8-
Descrição: dc.descriptionFAPESP: 2019/00137-2-
Descrição: dc.descriptionFAPESP: 2019/15428-2-
Descrição: dc.descriptionFAPESP: 2019/17058-8-
Descrição: dc.descriptionCNPq: 301279/2019-5-
Idioma: dc.languageen-
Relação: dc.relationLife Sciences-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAutophagy-
Palavras-chave: dc.subjectExercise-
Palavras-chave: dc.subjectLipogenesis-
Palavras-chave: dc.subjectLiver-
Palavras-chave: dc.subjectMTOR-
Título: dc.titleRapamycin did not prevent the excessive exercise-induced hepatic fat accumulation-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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