Proteomic analysis of infected root canals with apical periodontitis in patients with type 2 diabetes mellitus: A cross-sectional study

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.creatorLoureiro, Caroline-
Autor(es): dc.creatorBuzalaf, Marília Afonso Rabelo-
Autor(es): dc.creatorPessan, Juliano Pelim-
Autor(es): dc.creatorVentura, Talita Mendes Oliveira-
Autor(es): dc.creatorPelá, Vinícius Taioqui-
Autor(es): dc.creatorRibeiro, Ana Paula Fernandes-
Autor(es): dc.creatorJacinto, Rogério de Castilho-
Data de aceite: dc.date.accessioned2025-08-21T17:48:38Z-
Data de disponibilização: dc.date.available2025-08-21T17:48:38Z-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2022-09-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1111/iej.13794-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/241343-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/241343-
Descrição: dc.descriptionAim: This study aimed to quantitatively and qualitatively determine the proteomic profile of apical periodontitis (AP) in type 2 diabetes mellitus (T2DM) patients in comparison with systemically noncompromised patients and to correlate the protein expression of both groups with their biological functions. Methodology: The sample consisted of 18 patients with asymptomatic AP divided into two groups according to the presence of T2DM: diabetic group—patients with T2DM (n = 9) and control group—systemically healthy patients (n = 9). After sample collection, the root canal samples were prepared for proteomic analysis using reverse-phase liquid chromatography-mass spectrometry. Label-free quantitative proteomic analysis was performed by Protein Lynx Global Service software. Differences in protein expression between groups were calculated using t-test (p <.05). Biological functions were analysed using the Homo sapiens UniProt database. Results: A total of 727 human proteins were identified in all samples. Among them, 124 proteins common to both groups were quantified, out of which 65 proteins from the diabetic group showed significant differences compared with the control: 43 upregulated (p <.05) and 22 downregulated (p <.05) proteins. No significant differences in protein expression were seen for the remaining 59 proteins (p >.05). Most proteins with differences in expression were related to immune/inflammatory response. Neutrophil gelatinase-associated lipocalin, Plastin-2, Lactotransferrin and 13 isoforms of immunoglobulins were upregulated. In contrast, Protein S100-A8, Protein S100-A9, Histone H2B, Neutrophil defensin 1, Neutrophil defensin 3 and Prolactin-inducible protein were downregulated. Conclusions: Quantitative differences were demonstrated in the expression of proteins common to diabetic and control groups, mainly related to immune response, oxidative stress, apoptosis and proteolysis. These findings revealed biological pathways that provide the basis to support clinical findings on the relationship between AP and T2DM.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Preventive and Restorative Dentistry School of Dentistry Araçatuba São Paulo State University-
Descrição: dc.descriptionDepartment of Biological Sciences Bauru School of Dentistry University of São Paulo-
Descrição: dc.descriptionDepartment of Genetics and Evolution Federal University of Sao Carlos-
Descrição: dc.descriptionDepartment of Preventive and Restorative Dentistry School of Dentistry Araçatuba São Paulo State University-
Descrição: dc.descriptionCAPES: 001-
Descrição: dc.descriptionFAPESP: 2018/18741-0-
Descrição: dc.descriptionFAPESP: 2019/14995-0-
Formato: dc.format910-922-
Idioma: dc.languageen-
Relação: dc.relationInternational Endodontic Journal-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectapical periodontitis-
Palavras-chave: dc.subjectdiabetes mellitus-
Palavras-chave: dc.subjectendodontics-
Palavras-chave: dc.subjecthost–pathogen interactions-
Palavras-chave: dc.subjectproteomics-
Título: dc.titleProteomic analysis of infected root canals with apical periodontitis in patients with type 2 diabetes mellitus: A cross-sectional study-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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