Bioreactivity of a novel poly(epsilon-caprolactone) nanocapsule containing atrazine with human lung alveolar epithelial cells

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorImperial College London-
Autor(es): dc.contributorHealth Impact of Environmental Hazards at King's College London in partnership with Public Health England (PHE) in collaboration with Imperial College London-
Autor(es): dc.contributorUniversity of Sorocaba-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorMoore, Amber J. S.-
Autor(es): dc.creatorDean, Lareb S. N.-
Autor(es): dc.creatorYam, Aiken N. K.-
Autor(es): dc.creatorde Lima, Renata-
Autor(es): dc.creatorFraceto, Leonardo F.-
Autor(es): dc.creatorTetley, Teresa D.-
Data de aceite: dc.date.accessioned2025-08-21T15:19:40Z-
Data de disponibilização: dc.date.available2025-08-21T15:19:40Z-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2022-04-28-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1039/d1en01068a-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/241270-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/241270-
Descrição: dc.descriptionAtrazine (ATZ), a commonly used agricultural herbicide, is potentially harmful to animals and humans. Nanoencapsulation of ATZ (NC-ATZ) within non-toxic, biodegradable poly(ϵ-caprolactone) (PCL) improves the herbicidal activity of ATZ 10-fold and moderates its environmental persistence, possibly reducing off-target ecological effects. These compounds may reach the pulmonary respiratory units following inhalation; thus, we investigated the effect of ATZ and NC-ATZ on an immortalised human lung alveolar type 1-like epithelial cell model (TT1 cells). The concentration-dependent effect of the compounds was analysed by assessment of viability, reactive oxygen species (ROS) production and inflammatory cytokine release. Confocal microscopy was used to visualise TT1 cell-nanoparticle interactions. NC-ATZ caused cellular effects not observed with ATZ or the PCL nanocapsule alone. NC-ATZ significantly increased lactate dehydrogenase (LDH) release at ≥1 μg ml−1 after 48 hours of exposure, peaking at 5 μg ml−1 (3-fold of the non-treated control, ***p < 0.001). Moreover, ≥1 μg ml−1 NC-ATZ was pro-inflammatory at 48 hours, peaking at 5 μg ml−1 (IL-6 release ∼125 pg ml−1; IL-8 release ∼46 pg ml−1). Confocal microscopy of fluorescently-labelled ATZ and NC-ATZ indicated high intensity fluorescence nanoparticle uptake into the cytoplasm and co-localisation in the Golgi, suggesting nanoparticle recycling within 24 hours. We provide evidence that nanoencapsulation of the pesticide ATZ alters bioreactivity, stimulating more necrosis and inflammation in human lung alveolar type 1 epithelial cells in comparison to ATZ or the PCL nanocapsule alone. However, nanoencapsulation improves the efficacy of pesticides, thus lower pesticide concentrations could be used, reducing environmental contamination. Further investigation, particularly with different exposure scenarios, is warranted in order to generate safer nanoencapsulated pesticides.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionHealth Research-
Descrição: dc.descriptionPublic Health England-
Descrição: dc.descriptionUniversity of Kentucky-
Descrição: dc.descriptionUniverzita Karlova v Praze-
Descrição: dc.descriptionImperial College London-
Descrição: dc.descriptionLung Cell Biology Airways Disease National Heart and Lung Institute Imperial College London-
Descrição: dc.descriptionThe National Institute for Health Research Health Protection Research Unit (NIHR HPRU) Health Impact of Environmental Hazards at King's College London in partnership with Public Health England (PHE) in collaboration with Imperial College London-
Descrição: dc.descriptionLABiToN − Laboratory for Evaluation of Bioactivity and Toxicology of Nanomaterials University of Sorocaba-
Descrição: dc.descriptionSão Paulo State University (UNESP) Institute of Science and Technology of Sorocaba Laboratory of Environmental Nanotechnology, Sorocaba-
Descrição: dc.descriptionSão Paulo State University (UNESP) Institute of Science and Technology of Sorocaba Laboratory of Environmental Nanotechnology, Sorocaba-
Formato: dc.format2134-2148-
Idioma: dc.languageen-
Relação: dc.relationEnvironmental Science: Nano-
???dc.source???: dc.sourceScopus-
Título: dc.titleBioreactivity of a novel poly(epsilon-caprolactone) nanocapsule containing atrazine with human lung alveolar epithelial cells-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.