Comparison of two microscopic interpretations of vaginal microbiota with molecular profiling

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Federal do Paraná (UFPR)-
Autor(es): dc.contributorUniversity of Maryland School of Medicine-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorDurski, Mariana-
Autor(es): dc.creatorRavel, Jacques-
Autor(es): dc.creatorSpautz, Ana C.P.G.-
Autor(es): dc.creatorCarvalho, Newton S.-
Autor(es): dc.creatorSilva, Márcia G.-
Autor(es): dc.creatorMarconi, Camila-
Data de aceite: dc.date.accessioned2025-08-21T17:12:55Z-
Data de disponibilização: dc.date.available2025-08-21T17:12:55Z-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2022-09-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.diagmicrobio.2022.115728-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/241255-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/241255-
Descrição: dc.descriptionObjective: To compare the diagnosis of bacterial vaginosis (BV) by Nugent scoring criteria (Nugent-BV) and the diagnosis of BV and/or aerobic vaginitis (AV) using Donders criteria (Donders-BV/AV) for identifying Molecular-BV detected by bacterial 16s rRNA profiling. Methods: We enrolled 512 women of reproductive age in Brazil with data available on Nugent and Donders microscopic analysis and 16S rRNA sequencing. We constructed receiver operating characteristic (ROC) curves of Nugent-BV and Donders-BV/AV and calculated their area under the curves (AUCs) and 95% confidence interval (CI) for matching Molecular-BV. Results: A total of 155 (28.7%) participants were positive for Nugent-BV. Donders-BV and -AV were detected in 90 (17.6%) and 75 (14.6%) participants, respectively, while 28 (5.5%) had concurrent Donders-BV and -AV. Molecular-BV was identified in 139 (27.1%) participants. Analysis of ROC curves showed that diagnosis of Nugent-BV more accurately aligned with presence of Molecular-BV (AUC: 0.88, 95% CI: 0.84–0.91) when compared to Donders-AV/BV (AUC: 0.84; CI: 0.80–0.87) (P = 0.005). Conclusion: The use of Nugent-BV is more representative of Molecular-BV than Donders-AV/BV.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionPrograma de Pós-Graduação em Tocoginecologia e Saúde da Mulher University of Paraná (UFPR), Paraná-
Descrição: dc.descriptionInstitute for Genome Sciences Department of Microbiology and Immunology University of Maryland School of Medicine-
Descrição: dc.descriptionUniversity of Paraná (UFPR) Sector of Medical Sciences Department of Gynecology and Obstetrics, Paraná-
Descrição: dc.descriptionSão Paulo State University (UNESP) Botucatu Medical School Department of Pathology, São Paulo-
Descrição: dc.descriptionFederal University of Paraná (UFPR) Sector of Biological Sciences Department of Basic Pathology, Paraná-
Descrição: dc.descriptionSão Paulo State University (UNESP) Botucatu Medical School Department of Pathology, São Paulo-
Descrição: dc.descriptionFAPESP: 2012/10403-2-
Descrição: dc.descriptionFAPESP: 2012/16800-3-
Descrição: dc.descriptionFAPESP: 2014/22463-5-
Idioma: dc.languageen-
Relação: dc.relationDiagnostic Microbiology and Infectious Disease-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subject16S rRNA gene-
Palavras-chave: dc.subjectaerobic vaginitis-
Palavras-chave: dc.subjectbacterial vaginosis-
Palavras-chave: dc.subjectmicroscopy-
Palavras-chave: dc.subjectmolecular diagnostics-
Palavras-chave: dc.subjectvaginal microbiota-
Título: dc.titleComparison of two microscopic interpretations of vaginal microbiota with molecular profiling-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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