Post-thaw dilution of Rhamdia quelen sperm improves the reproductive success

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorFederal University of Rio Grande do Sul-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorCatholic University of Temuco-
Autor(es): dc.contributorUFRGS-
Autor(es): dc.contributorUniversity of Vale of Taquari - Univates-
Autor(es): dc.creatorFrança, Thales de Souza-
Autor(es): dc.creatorGomes, Itamar Cossina-
Autor(es): dc.creatorSanches, Eduardo Antônio-
Autor(es): dc.creatorAtehortúa, Maritza Pérez-
Autor(es): dc.creatorTeixeira, Nathalia Santos-
Autor(es): dc.creatorRodrigues, Rômulo Batista-
Autor(es): dc.creatorde Freitas, Thaiza Rodrigues-
Autor(es): dc.creatorGaluppo, Andrea Giannotti-
Autor(es): dc.creatorQuirino, Monike-
Autor(es): dc.creatorBenato, Jhony Lisbôa-
Autor(es): dc.creatorMachado, Thales Lysakowski Flores-
Autor(es): dc.creatorMarques, Lis Santos-
Autor(es): dc.creatorBustamante-Filho, Ivan Cunha-
Autor(es): dc.creatorBortolozzo, Fernando Pandolfo-
Autor(es): dc.creatorStreit Jr, Danilo Pedro-
Data de aceite: dc.date.accessioned2025-08-21T17:03:04Z-
Data de disponibilização: dc.date.available2025-08-21T17:03:04Z-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2022-08-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.anireprosci.2022.107018-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/241177-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/241177-
Descrição: dc.descriptionThe aim was to evaluate the effect of a post-thaw dilution of Rhamdia quelen sperm in 1.1% NaCl (325 mOsm kg-1; pH 7.6; 24 °C) solution on the quality and reproductive capacity. Sperm from eight males were cryopreservation in nitrogen vapor at − 170 °C for 18 h in 0.25 mL straws in a freezing medium containing 5% fructose, 5% Powdered milk, and 10% methanol. The samples were thawed and post-thaw diluted (1:20) in NaCl solution or not (control). The higher spermatozoa velocities were observed in the post-thaw diluted samples (curvilinear (VCL) - 69 ± 11 µm s-1; average path (VAP) - 45 ± 8 µm s-1; straight-line (VSL) - 43 ± 8 µm s-1) compared to the control (VCL - 47 ± 10 µm s-1; VAP - 31 ± 6 µm s-1; VSL - 30 ± 6 µm s-1). Greater straightness (STR), progression (PROG), and beat cross frequency (BCF) were observed in the post-thaw diluted samples (STR - 96 ± 7%; PROG - 666 ± 128 µm; BCF - 42 ± 2 Hz) than in control (STR - 95 ± 5%; PROG - 463 ± 92 µm; BCF - 40 ± 2 Hz). The strongly curled tail was the only morphology change that differ between the post-thaw diluted (5 ± 2%) and control (2 ± 1%). Membrane integrity, mitochondrial activity, and normal larvae rate were not different between treatments. Fertilization and hatching were higher in the post-thaw diluted sperm (93 ± 3%; 82 ± 9%) when compared to control samples (65 ± 13%; 55 ± 17%). Were used oocytes from one female, limiting these results. The post-thaw dilution improved the sperm kinetics and reproductive parameters. Thus, this methodology can be included in the sperm cryopreservation protocol for R. quelen.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado do Rio Grande do Sul-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionAquam Research Group Animal Science Research Program Federal University of Rio Grande do Sul, Rio Grande do Sul-
Descrição: dc.descriptionFishery Engineering Course and Aquaculture Centre (CAUNESP) São Paulo State University, São Paulo-
Descrição: dc.descriptionDepartment of Agricultural and Aquacultural Sciences Faculty of Natural Resources Catholic University of Temuco-
Descrição: dc.descriptionDept. Veterinary Medicine Federal University of Rio Grande do Sul UFRGS, Rio Grande do Sul-
Descrição: dc.descriptionBiotechnology Laboratory University of Vale of Taquari - Univates, Avelio Tallini Street, 171-
Descrição: dc.descriptionFishery Engineering Course and Aquaculture Centre (CAUNESP) São Paulo State University, São Paulo-
Descrição: dc.descriptionCNPq: 141717/2019-0-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado do Rio Grande do Sul: 17/2551-0000917-7-
Descrição: dc.descriptionCAPES: 88882.346090/2015-1-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado do Rio Grande do Sul: FAPERGS-
Idioma: dc.languageen-
Relação: dc.relationAnimal Reproduction Science-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectArtificial fertilization-
Palavras-chave: dc.subjectHatching-
Palavras-chave: dc.subjectSouth American silver catfish-
Palavras-chave: dc.subjectSperm cryopreservation-
Palavras-chave: dc.subjectSperm dilution-
Palavras-chave: dc.subjectSperm quality-
Título: dc.titlePost-thaw dilution of Rhamdia quelen sperm improves the reproductive success-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.