Evaluation of photosensitizer-containing superhydrophobic surfaces for the antibacterial treatment of periodontal biofilms

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Autor(es): dc.contributorMassachusetts General Hospital and Harvard Medical School-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorHarvard University and Massachusetts Institute of Technology-
Autor(es): dc.contributorSingletO2 Therapeutics LLC-
Autor(es): dc.contributorCity University of New York-
Autor(es): dc.contributorThe Graduate Center of the City University of New York-
Autor(es): dc.creatorTonon, Caroline Coradi-
Autor(es): dc.creatorAshraf, Shoaib-
Autor(es): dc.creatorde Souza Rastelli, Alessandra Nara-
Autor(es): dc.creatorGhosh, Goutam-
Autor(es): dc.creatorHasan, Tayyaba-
Autor(es): dc.creatorXu, QianFeng-
Autor(es): dc.creatorGreer, Alexander-
Autor(es): dc.creatorLyons, Alan M.-
Data de aceite: dc.date.accessioned2025-08-21T21:28:31Z-
Data de disponibilização: dc.date.available2025-08-21T21:28:31Z-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2022-08-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.jphotobiol.2022.112458-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/241128-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/241128-
Descrição: dc.descriptionAntimicrobial photodynamic therapy (aPDT) is a promising approach to control biofilms involved in periodontal diseases. However, certain challenges, such as staining of teeth, preferential interaction of photosensitizer (PS) with Gram-positive versus Gram-negative bacteria, and insufficient oxygen in hypoxic periodontal pockets have presented barriers to its use in the clinic. To overcome these challenges, a novel superhydrophobic (SH) film that generates airborne singlet oxygen has been developed. The SH-aPDT approach isolates the PS onto a topologically rough solid SH film on which channels allow air to diffuse to the PS surface, thus ensuring sufficient oxygen supply. Upon illumination, gas phase singlet oxygen (1O2) is produced and diffuses from the SH surface to the underlying biofilm. The killing efficacy was assessed as a function of transmitted fluence (17.9–89.5 J/cm2) and chorin e6 loading (96–1110 nmol/cm2) by counting of colony forming units, biofilm metabolism by XTT and confocal microscopy. The decrease in viability of both Gram-positive and Gram-negative bacteria in a multi-species biofilm was found to be linearly dependent on the fluence as well as the loading of the PS up to 71.6 J/cm2 when 1110 nmols/cm2 of chlorin e6 was used. A > 4.6 log bacterial reduction was observed under these conditions (p < 0.05). This novel SH-aPDT approach shows promise as an effective method to disinfect multi-species bacterial biofilms associated with periodontal disease and will be evaluated in animal models in future studies.-
Descrição: dc.descriptionNational Institute of Dental and Craniofacial Research-
Descrição: dc.descriptionWellman Center for Photomedicine Massachusetts General Hospital and Harvard Medical School, 40 Blossom St-
Descrição: dc.descriptionDepartment of Restorative Dentistry School of Dentistry Araraquara São Paulo State University-UNESP, 1680 Humaitá St.-
Descrição: dc.descriptionDivision of Health Sciences and Technology Harvard University and Massachusetts Institute of Technology-
Descrição: dc.descriptionSingletO2 Therapeutics LLC, TechBox, Suite 3, 75 Clinton St-
Descrição: dc.descriptionDepartment of Chemistry College of Staten Island City University of New York, Staten Island-
Descrição: dc.descriptionPh.D. Program in Chemistry The Graduate Center of the City University of New York, 365 Fifth Avenue-
Descrição: dc.descriptionDepartment of Chemistry Brooklyn College City University of New York-
Descrição: dc.descriptionDepartment of Restorative Dentistry School of Dentistry Araraquara São Paulo State University-UNESP, 1680 Humaitá St.-
Descrição: dc.descriptionNational Institute of Dental and Craniofacial Research: 2R44DE026083-03-
Idioma: dc.languageen-
Relação: dc.relationJournal of Photochemistry and Photobiology B: Biology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAntimicrobial photodynamic inactivation-
Palavras-chave: dc.subjectBacterial biofilms-
Palavras-chave: dc.subjectChlorin e6-
Palavras-chave: dc.subjectPeriodontal disease-
Palavras-chave: dc.subjectSinglet oxygen-
Palavras-chave: dc.subjectSuperhydrophobicity-
Título: dc.titleEvaluation of photosensitizer-containing superhydrophobic surfaces for the antibacterial treatment of periodontal biofilms-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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