Pracaxi oil affects xenobiotic metabolisms, cellular proliferation, and oxidative stress without cytotogenotoxic effects in HepG2/C3A cells

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade Estadual de Londrina (UEL)-
Autor(es): dc.contributorUniversity of Trás-os-Montes and Alto Douro (UTAD)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.creatorPires, Camila Lehnhardt-
Autor(es): dc.creatorZanetti, Thalita Alves-
Autor(es): dc.creatorMantovani, Mario Sergio-
Autor(es): dc.creatorGaivão, Isabel O'Neill de Mascarenhas-
Autor(es): dc.creatorPerazzo, Fábio Ferreira-
Autor(es): dc.creatorRosa, Paulo Cesar Pires-
Autor(es): dc.creatorMaistro, Edson Luis-
Data de aceite: dc.date.accessioned2025-08-21T21:33:28Z-
Data de disponibilização: dc.date.available2025-08-21T21:33:28Z-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2022-09-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.tiv.2022.105392-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/241117-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/241117-
Descrição: dc.descriptionPentaclethra macroloba (Willd.) Kuntze seeds oil has been used as a topical healing agent, applied mainly to parturients and snake bites. The objective was to investigate the effects of pracaxi oil (POP) on HepG2/C3A cells under cytogenotoxicity, cell cycle and apoptosis influence, and expression of metabolism and other related cell types proliferation genes. Cytotoxicity was analyzed by MTT test and apoptosis and cell cycle interferences by flow cytometry. To identify genotoxicity were used comet and micronucleus tests. RT-qPCR investigated gene expression. PO chemical characterization has shown two significant triterpenes, identified as oleanolic acid and hederagenin. The results showed that the PO did not reduce cell viability at concentrations ranging from 31 to 500 μg/ml. Comet and micronucleus assays revealed the absence of genotoxic effects, and flow cytometry showed no cell cycle or apoptosis disturbance. RT-qPCR indicated that PO up-regulated genes related to metabolism (CYP3A4, CYP1A2, CYP1A1), cell proliferation (mTOR), and oxidative stress (GPX1). The data indicate that PO has no cytogenotoxic effects and suggest that it activated the PI3/AKT/mTOR cascade of cell growth and proliferation. Inside the cells, the PO activated xenobiotic metabolizing genes, responsible for reactive oxygen species (ROS) generation, can neutralize ROS with increased GPX1 gene expression without genetic damage, interruption of the cell cycle, or induction of apoptosis.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionBiosciences Institute, São Paulo State University – UNESP, Botucatu-
Descrição: dc.descriptionDepartment of General Biology Biological Sciences Center Londrina State University - UEL, Londrina-
Descrição: dc.descriptionDepartment of Genetics Biotechnology and Animal and Veterinary Research Centre (CECAV) University of Trás-os-Montes and Alto Douro (UTAD), Vila Real-
Descrição: dc.descriptionInstitute of Environmental, Chemical and Pharmaceutical Sciences, Federal University of São Paulo, Diadema-
Descrição: dc.descriptionFaculty of Pharmaceutical Sciences University of Campinas, Campinas-
Descrição: dc.descriptionSpeech and Hearing Therapy Department São Paulo State University - UNESP, Marília-
Descrição: dc.descriptionSpeech and Hearing Therapy Department São Paulo State University - UNESP, Marília-
Descrição: dc.descriptionFAPESP: 2017/24149–4-
Descrição: dc.descriptionCNPq: 303604/2021-2-
Idioma: dc.languageen-
Relação: dc.relationToxicology in Vitro-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCell proliferation-
Palavras-chave: dc.subjectComet assay-
Palavras-chave: dc.subjectGene expression of CYP-
Palavras-chave: dc.subjectMicronucleus test-
Palavras-chave: dc.subjectmTOR-
Palavras-chave: dc.subjectPentaclethra macroloba seed oil-
Título: dc.titlePracaxi oil affects xenobiotic metabolisms, cellular proliferation, and oxidative stress without cytotogenotoxic effects in HepG2/C3A cells-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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