Caffeine and Chlorogenic Acid Combination Attenuate Early-Stage Chemically Induced Colon Carcinogenesis in Mice: Involvement of oncomiR miR-21a-5p

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversity of Granada-
Autor(es): dc.contributorPfizer/University of Granada/Andalusian Regional Government-
Autor(es): dc.contributorComplejo Hospitalario de Jaen-
Autor(es): dc.contributorSapienza University of Rome-
Autor(es): dc.creatorBartolomeu, Ariane Rocha-
Autor(es): dc.creatorRomualdo, Guilherme Ribeiro-
Autor(es): dc.creatorLisón, Carmen Griñán-
Autor(es): dc.creatorBesharat, Zein Mersini-
Autor(es): dc.creatorCorrales, Juan Antonio Marchal-
Autor(es): dc.creatorChaves, Maria Ángel García-
Autor(es): dc.creatorBarbisan, Luís Fernando-
Data de aceite: dc.date.accessioned2025-08-21T21:59:20Z-
Data de disponibilização: dc.date.available2025-08-21T21:59:20Z-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2022-06-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/ijms23116292-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/241091-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/241091-
Descrição: dc.descriptionColorectal cancer (CRC) is one of most common cancers worldwide, with high rates of mortality. Epidemiological findings demonstrate that coffee consumption reduces the risk of developing CRC by ~13%. In general, in vivo and in vitro findings demonstrate the antiproliferative, antioxidant and proapoptotic effects of brewed coffee or major bioavailable coffee compounds. Thus, it was assessed whether caffeine (CAF) and/or chlorogenic acid (CGA) attenuates the early-stage of chemically induced mouse colon carcinogenesis. Male Swiss mice were submitted to a 1,2-dimethylhydrazine/deoxycholic acid (DMH/DCA)-induced colon carcinogenesis model. These animals received CAF (50 mg/kg), CGA (25 mg/kg) or CAF+CGA (50 + 25 mg/kg) intragastrically for five times/week for ten weeks. CAF+CGA had the most pronounced effects on decreasing epithelial cell proliferation (Ki-67) and increasing apoptosis (cleaved caspase-3) in colonic crypts. This treatment also decreased the levels of proinflammatory cytokines IL-6, IL-17 and TNF-α, and downregulated the oncomiR miR-21a-5p in the colon. Accordingly, the analysis of miR-21a-5p targets demonstrated the genes involved in the negative regulation of proliferation and inflammation, and the positive regulation of apoptosis. Ultimately, CAF+CGA attenuated preneoplastic aberrant crypt foci (ACF) development. Our findings suggest that a combination of coffee compounds reduces early-stage colon carcinogenesis by the modulation of miR-21a-5p expression, highlighting the importance of coffee intake to prevent CRC.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Pathology Botucatu Medical School São Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Structural and Functional Biology Biosciences Institute São Paulo State University (UNESP), SP-
Descrição: dc.descriptionBiosanitary Research Institute of Granada (ibs.GRANADA) University Hospitals of Granada University of Granada-
Descrição: dc.descriptionGENYO (Centre for Genomics and Oncological Research) Pfizer/University of Granada/Andalusian Regional Government-
Descrição: dc.descriptionUGC de Oncología Médica Complejo Hospitalario de Jaen-
Descrição: dc.descriptionDepartment of Experimental Medicine Sapienza University of Rome-
Descrição: dc.descriptionDepartment of Human Anatomy and Embryology School of Medicine University of Granada-
Descrição: dc.descriptionDepartment of Biochemistry and Molecular Biology III and Immunology University of Granada-
Descrição: dc.descriptionDepartment of Pathology Botucatu Medical School São Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Structural and Functional Biology Biosciences Institute São Paulo State University (UNESP), SP-
Descrição: dc.descriptionFAPESP: #2016/12015–0-
Descrição: dc.descriptionFAPESP: #2016/14420–0-
Descrição: dc.descriptionFAPESP: #2017/26217-7-
Idioma: dc.languageen-
Relação: dc.relationInternational Journal of Molecular Sciences-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectcaffeine-
Palavras-chave: dc.subjectchemically induced colon carcinogenesis-
Palavras-chave: dc.subjectchlorogenic acid-
Palavras-chave: dc.subjectmice-
Palavras-chave: dc.subjectmiRNA expression-
Título: dc.titleCaffeine and Chlorogenic Acid Combination Attenuate Early-Stage Chemically Induced Colon Carcinogenesis in Mice: Involvement of oncomiR miR-21a-5p-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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