Cervicovaginal loads of Gardnerella spp. are increased in immunocompetent women with persistent high-risk human papillomavirus infection

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversity of Beira Interior-
Autor(es): dc.contributorLabfit-HPRD: Health Products Research and Development Lda-
Autor(es): dc.contributorUniversidade Federal do Paraná (UFPR)-
Autor(es): dc.creatorBelleti, Rafael-
Autor(es): dc.creatorMarcolino, Larissa Doddi-
Autor(es): dc.creatorNovak, Juliano-
Autor(es): dc.creatorFerreira, Carolina Sanitá Tafner-
Autor(es): dc.creatorBolpetti, Aline do Nascimento-
Autor(es): dc.creatorPinto, Gabriel Vitor da Silva-
Autor(es): dc.creatorOliveira, Ana Palmeira de-
Autor(es): dc.creatorSilva, Márcia Guimarães da-
Autor(es): dc.creatorMarconi, Camila-
Data de aceite: dc.date.accessioned2025-08-21T15:44:55Z-
Data de disponibilização: dc.date.available2025-08-21T15:44:55Z-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2022-05-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1099/jmm.0.001527-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/240993-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/240993-
Descrição: dc.descriptionIntroduction. Two high-oncogenic-risk human papilomavirus (hrHPV) genotypes - HPV16 and HPV18 - cause most of the cases of cervical cancer worldwide. Bacterial vaginosis is associated with increased hrHPV persistence, although the mechanism underlying this association remains unclear. Gardnerella spp. are detected in nearly all cases of bacterial vaginosis and are the major source of cervicovaginal sialidases. The NanH1 gene is present in virtually all Gardnerella sialidase-producing strains and has been proposed as a potential marker for persistent hrHPV infection.Hypothesis.Gardnerella spp. load and the NanH1 gene are associated with hrHPV persistence.Aim. To compare the cervicovaginal load of Gardnerella spp. and the frequency of the NanH1 gene between women with persistent HPV16 and/or HPV18 infection and those who cleared the infection after 11 months.Methodology. Among a population of 1638 HPV screened, we detected 104 with positive HPV16 and/or HPV18 results. Samples were obtained at two time points (baseline and at a median of 11 months at follow-up) and tested using the Linear Array HPV Genotyping kit (Roche Molecular Systems, Pleasanton, CA, USA). Based on their HPV16/HPV18 status at enrolment and follow-up, participants were assigned to 'persistence' or 'clearance' groups. We used cervicovaginal fluid samples obtained upon enrolment to determine the load of the 23 s rRNA gene of Gardnerella spp. and the presence of the NanH1 gene using real-time polymerase chain reaction (PCR). We compared Gardnerella spp. loads and NanH1 frequency between the groups by, respectively, Mann-Whitney and chi-squared tests, with a P-value <0.05 considered to be significant.Results. Of the 104 participants who were positive for HPV16/HPV18, 73 (70.2 %) persisted with at least 1 of the baseline genotypes at follow-up, while 31 (29.8 %) cleared the infection in this time frame. Participants in the persistence group had significantly higher loads of Gardnerella spp. [5.8E+02 (0-3.0E+05) copies µl-1] than those in the clearance group [9.9E+01 (0-7.7E+04) copies µl-1] (P=0.03). The baseline frequency of NanH1 was higher in the persistence' (n=46, 63.0 %) than in the clearance (n=14, 45.2 %) group, although this was not statistically significant (P=0.09).Conclusion. These findings reinforce the negative effect of vaginal microbiota for the clearance of hrHPV and indicate a possible association between sialidase-producing species with hrHPV persistence.-
Descrição: dc.descriptionDepartment of Pathology Botucatu Medical School UNESP São Paulo State University-
Descrição: dc.descriptionHealth Sciences Research Center (CICS-UBI) Faculty of Health Sciences University of Beira Interior-
Descrição: dc.descriptionLabfit-HPRD: Health Products Research and Development Lda-
Descrição: dc.descriptionDepartment of Basic Pathology Setor de Ciências Biológicas UFPR Universidade Federal do Paraná-
Descrição: dc.descriptionDepartment of Pathology Botucatu Medical School UNESP São Paulo State University-
Idioma: dc.languageen-
Relação: dc.relationJournal of medical microbiology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectclearance-
Palavras-chave: dc.subjectGardnerella-
Palavras-chave: dc.subjecthigh risk HPV-
Palavras-chave: dc.subjectHPV-
Palavras-chave: dc.subjectpersistence-
Palavras-chave: dc.subjectsialidase-
Palavras-chave: dc.subjectvaginal microbiota-
Título: dc.titleCervicovaginal loads of Gardnerella spp. are increased in immunocompetent women with persistent high-risk human papillomavirus infection-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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