A Sporothrix spp. enolase derived multi-epitope vaccine confers protective response in BALB/c mice challenged with Sporothrix brasiliensis

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorGC01 Immunology and Allergy Group. Maimonides Biomedical Research Institute of Cordoba (IMIBIC). Reina Sofía University Hospital-
Autor(es): dc.contributorPontificia Universidad Católica de Valparaíso-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidad Autónoma de Chile-
Autor(es): dc.creatorPortuondo, Deivys Leandro-
Autor(es): dc.creatorBatista-Duharte, Alexander-
Autor(es): dc.creatorCardenas, Constanza-
Autor(es): dc.creatorOliveira, Carlos S. de-
Autor(es): dc.creatorBorges, Júlio César-
Autor(es): dc.creatorTéllez-Martínez, Damiana-
Autor(es): dc.creatorSantana, Paula Andrea-
Autor(es): dc.creatorGauna, Adriana-
Autor(es): dc.creatorMercado, Luis-
Autor(es): dc.creatorCastilho, Bruna Mateus de-
Autor(es): dc.creatorCosta, Paulo-
Autor(es): dc.creatorGuzmán, Fanny-
Autor(es): dc.creatorCarlos, Iracilda Zeppone-
Data de aceite: dc.date.accessioned2025-08-21T17:41:02Z-
Data de disponibilização: dc.date.available2025-08-21T17:41:02Z-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2022-05-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.micpath.2022.105539-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/240910-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/240910-
Descrição: dc.descriptionSporotrichosis is a cosmopolitan mycosis caused by pathogenic species of Sporothrix genus, that in Brazil is often acquired by zoonotic transmission involved infected cats with S. brasiliensis. Previous studies showed that the Sporothrix spp. recombinant enolase (rSsEno), a multifunctional protein with immunogenic properties, could be a promising target for vaccination against sporotrichosis in cats. Nevertheless, the considerable sequence identity (62%) of SsEno with its feline counterpart is a great concern. Here, we report the identification in silico, chemical synthesis and biological validation of six peptides of SsEno with low sequence identity to its cat orthologue. All synthesized peptides exhibit B-cell epitopes on the molecular surface of SsEno and proved to be highly reactive with the serum of infected mice with S. brasiliensis and sera of cats with sporotrichosis. Interestingly, our study revealed that anti-peptide sera did not react with the recombinant enolase from Felis catus (cats, rFcEno), thus, may not trigger autoimmune response in these felines if used as a vaccine antigen. The immunization with peptide mixture (PeptMix) formulated with Freund adjuvant (FA), induced high levels of antigen-specific IgG, IgG1 and IgG2b antibodies that conferred protection upon passive transference in infected BALB/c mice with S. brasiliensis. We also observed, that the FA+PeptMix formulation induced a Th1/Th2/Th17 cytokine profile ex vivo, associated with protecting effect against the experimental sporotrichosis. Our results suggest that the six SsEno-derived peptides here evaluated, could be used as safe antigens for the development of vaccine strategies against feline sporotrichosis, whether prophylactic or therapeutic.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Pharmaceutical Sciences Department of Clinical Analysis, SP-
Descrição: dc.descriptionGC01 Immunology and Allergy Group. Maimonides Biomedical Research Institute of Cordoba (IMIBIC). Reina Sofía University Hospital, IMIBIC Building-
Descrição: dc.descriptionNúcleo de Biotecnología Curauma (NBC) Pontificia Universidad Católica de Valparaíso, Campus Curauma-
Descrição: dc.descriptionSão Carlos Institute of Chemistry University of São Paulo, P.O. Box 780, SP-
Descrição: dc.descriptionFacultad de Ingeniería Instituto de Ciencias Químicas Aplicadas Universidad Autónoma de Chile, el Llano Subercaseaux, Santiago-
Descrição: dc.descriptionGrupo de Marcadores Inmunológicos Laboratorio de Genética e Inmunología Molecular Instituto de Biología Pontificia Universidad Católica de Valparaíso, Avenida Universidad #330-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Pharmaceutical Sciences Department of Clinical Analysis, SP-
Descrição: dc.descriptionFAPESP: 2015/09340–4-
Descrição: dc.descriptionFAPESP: 2017/13228–0-
Descrição: dc.descriptionFAPESP: 2017/26774–3-
Idioma: dc.languageen-
Relação: dc.relationMicrobial Pathogenesis-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectEnolase-
Palavras-chave: dc.subjectFelis catus-
Palavras-chave: dc.subjectPeptide vaccine-
Palavras-chave: dc.subjectSporothrix brasiliensis-
Palavras-chave: dc.subjectSporotrichosis-
Título: dc.titleA Sporothrix spp. enolase derived multi-epitope vaccine confers protective response in BALB/c mice challenged with Sporothrix brasiliensis-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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