Neuroprotective effects on microglia and insights into the structure–activity relationship of an antioxidant peptide isolated from Pelophylax perezi

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Autor(es): dc.contributorUniversity of Porto-
Autor(es): dc.contributorUniversidade de Lisboa-
Autor(es): dc.contributorUniversity of Brasilia-
Autor(es): dc.contributorUniversity of Brasília-
Autor(es): dc.contributorFederal University of Delta do Parnaíba (UFDPar)-
Autor(es): dc.contributorScience and Technology of São Paulo-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorCRL-
Autor(es): dc.contributorUniversity of Lincoln-
Autor(es): dc.creatorPlácido, Alexandra-
Autor(es): dc.creatorAmaral, Constança do Pais do-
Autor(es): dc.creatorTeixeira, Cátia-
Autor(es): dc.creatorNogueira, Ariane-
Autor(es): dc.creatorBrango-Vanegas, José-
Autor(es): dc.creatorBarbosa, Eder Alves-
Autor(es): dc.creatorMoreira, Daniel C.-
Autor(es): dc.creatorCarvalho, Amandda É. Silva-
Autor(es): dc.creatorSilva, Maria da Gloria da-
Autor(es): dc.creatorDias, Jhones do Nascimento-
Autor(es): dc.creatorAlbuquerque, Patrícia-
Autor(es): dc.creatorSaldanha-Araújo, Felipe-
Autor(es): dc.creatorLima, Filipe C. D. A.-
Autor(es): dc.creatorBatagin-Neto, Augusto-
Autor(es): dc.creatorKuckelhaus, Selma-
Autor(es): dc.creatorBessa, Lucinda J.-
Autor(es): dc.creatorFreitas, Jaime-
Autor(es): dc.creatorDotto Brand, Guilherme-
Autor(es): dc.creatorSantos, Nuno C.-
Autor(es): dc.creatorRelvas, João B.-
Autor(es): dc.creatorGomes, Paula-
Autor(es): dc.creatorLeite, José Roberto S. A.-
Autor(es): dc.creatorEaton, Peter-
Data de aceite: dc.date.accessioned2025-08-21T16:34:05Z-
Data de disponibilização: dc.date.available2025-08-21T16:34:05Z-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2022-05-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1111/jcmm.17292-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/240882-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/240882-
Descrição: dc.descriptionTryptophyllins constitute a heterogeneous group of peptides that are one of the first classes of peptides identified from amphibian’s skin secretions. Here, we report the structural characterization and antioxidant properties of a novel tryptophyllin-like peptide, named PpT-2, isolated from the Iberian green frog Pelophylax perezi. The skin secretion of P. perezi was obtained by electrical stimulation and fractionated using RP-HPLC. De novo peptide sequencing was conducted using MALDI MS/MS. The primary structure of PpT-2 (FPWLLS-NH2) was confirmed by Edman degradation and subsequently investigated using in silico tools. PpT-2 shared physicochemical properties with other well-known antioxidants. To test PpT-2 for antioxidant activity in vitro, the peptide was synthesized by solid phase and assessed in the chemical-based ABTS and DPPH scavenging assays. Then, a flow cytometry experiment was conducted to assess PpT-2 antioxidant activity in oxidatively challenged murine microglial cells. As predicted by the in silico analyses, PpT-2 scavenged free radicals in vitro and suppressed the generation of reactive species in PMA-stimulated BV-2 microglia cells. We further explored possible bioactivities of PpT-2 against prostate cancer cells and bacteria, against which the peptide exerted a moderate antiproliferative effect and negligible antimicrobial activity. The biocompatibility of PpT-2 was evaluated in cytotoxicity assays and in vivo toxicity with Galleria mellonella. No toxicity was detected in cells treated with up to 512 µg/ml and in G. mellonella treated with up to 40 mg/kg PpT-2. This novel peptide, PpT-2, stands as a promising peptide with potential therapeutic and biotechnological applications, mainly for the treatment/prevention of neurodegenerative disorders.-
Descrição: dc.descriptionDepartment of Chemistry and Biochemistry LAQV/REQUIMTE Faculty of Sciences University of Porto-
Descrição: dc.descriptionInstituto de Medicina Molecular Faculdade de Medicina Universidade de Lisboa-
Descrição: dc.descriptionCenter for Research in Applied Morphology and Immunology (NuPMIA) University of Brasilia-
Descrição: dc.descriptionLaboratory of Synthesis and Analysis of Biomolecules (LSAB) Institute of Chemistry (IQ) University of Brasilia-
Descrição: dc.descriptionLaboratory of Hematology and Stem Cells Faculty of Health Sciences University of Brasilia-
Descrição: dc.descriptionDepartment of Cell Biology Institute of Biological Sciences University of Brasília-
Descrição: dc.descriptionBiomedicine Course Federal University of Delta do Parnaíba (UFDPar)-
Descrição: dc.descriptionFaculty of Ceilândia University of Brasilia-
Descrição: dc.descriptionFederal Institute of Education Science and Technology of São Paulo-
Descrição: dc.descriptionSão Paulo State University (UNESP) Campus of Itapeva, SP-
Descrição: dc.descriptionEgas Moniz Interdisciplinary Research Center (CiiEM) Egas Moniz - Cooperative for Higher Education CRL-
Descrição: dc.descriptionInstitute for Research and Innovation in Health (i3S) National Institute of Biomedical Engineering (INEB) University of Porto-
Descrição: dc.descriptionInstitute for Research and Innovation in Health (i3S) Institute for Molecular and Cell Biology (IBMC) University of Porto-
Descrição: dc.descriptionThe Bridge School of Chemistry Joseph Banks Laboratories University of Lincoln-
Descrição: dc.descriptionSão Paulo State University (UNESP) Campus of Itapeva, SP-
Formato: dc.format2793-2807-
Idioma: dc.languageen-
Relação: dc.relationJournal of Cellular and Molecular Medicine-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAmphibia-
Palavras-chave: dc.subjectantioxidant-
Palavras-chave: dc.subjectbioactive peptide-
Palavras-chave: dc.subjectneuroprotection-
Palavras-chave: dc.subjectPelophylax perezi-
Palavras-chave: dc.subjecttryptophyllin-
Título: dc.titleNeuroprotective effects on microglia and insights into the structure–activity relationship of an antioxidant peptide isolated from Pelophylax perezi-
Tipo de arquivo: dc.typelivro digital-
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