Early infection of Zika virus in the male reproductive system of AG129 mice: molecular and immunohistochemical evaluation

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorRondonópolis Federal University (UFR)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorSão José Do Rio Preto-
Autor(es): dc.creatorLima, Maria Letícia Duarte-
Autor(es): dc.creatorCabral, Ágata Silva-
Autor(es): dc.creatorBittar, Cintia-
Autor(es): dc.creatorFalleiros Junior, Luiz Roberto-
Autor(es): dc.creatorGuerra, Luiz Henrique Alves-
Autor(es): dc.creatorCarneiro, Bruno Moreira-
Autor(es): dc.creatorFerreira, Luis Carlos de Souza-
Autor(es): dc.creatorNogueira, Maurício Lacerda-
Autor(es): dc.creatorTaboga, Sebastião Roberto-
Autor(es): dc.creatorCalmon, Marilia Freitas-
Autor(es): dc.creatorRahal, Paula-
Data de aceite: dc.date.accessioned2025-08-21T17:35:45Z-
Data de disponibilização: dc.date.available2025-08-21T17:35:45Z-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2021-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s42770-022-00761-x-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/240876-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/240876-
Descrição: dc.descriptionSexual transmission of Zika virus (ZIKV), an important arbovirus, and the virus persistence in semen raise several questions about how and where it circulates in the male reproductive system (MRS). Several studies reported detection of the virus in testes, epididymis, and prostate at 5 days post-infection (dpi) or more in animal models. In the present study, we investigated the interactions of ZIKV with mouse MRS using the AG129 strain, a ZIKV permissive immunodeficient mouse strain, at two dpi. Viral RNA was detected in blood, testes, epididymis, and prostatic complexes (prostate and seminal vesicles). Immunohistochemical (IHC) analyses, based on the envelope protein, showed an early infection in organs of MRS since ZIKV positive antigens were detected in cells within or surrounding blood vessels, Sertoli, and germ cells in testes and epithelial cells in epididymis and prostate. Positive antigens for NS5 protein, the virus RNA-dependent RNA polymerase, were also detected by IHC in these organs and circulating leukocytes, suggesting that the virus replicates in these sites as early as 2 days post-infection. Analysis of the early stages of ZIKV infection in MRS may improve the current knowledge about this issue and contribute to the development of therapies directed to the infection at this site.-
Descrição: dc.descriptionDepartment of Biology Institute of Biosciences Humanities and Exact Sciences São Paulo State University (UNESP) São José Do Rio Preto-
Descrição: dc.descriptionInstitute of Exact and Natural Science Rondonópolis Federal University (UFR), Mato Grosso-
Descrição: dc.descriptionDepartment of Microbiology Institute of Biomedical Science São Paulo University (USP)-
Descrição: dc.descriptionDepartment of Infectious Dermatological and Parasitic Infections São José Do Rio Preto Medical School (FAMERP) São José Do Rio Preto-
Descrição: dc.descriptionDepartment of Biology Institute of Biosciences Humanities and Exact Sciences São Paulo State University (UNESP) São José Do Rio Preto-
Idioma: dc.languageen-
Relação: dc.relationBrazilian Journal of Microbiology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAG129-
Palavras-chave: dc.subjectMale reproductive system-
Palavras-chave: dc.subjectMice-
Palavras-chave: dc.subjectZika virus-
Palavras-chave: dc.subjectZIKV-
Título: dc.titleEarly infection of Zika virus in the male reproductive system of AG129 mice: molecular and immunohistochemical evaluation-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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