Advancing respiratory–cardiovascular physiology with the working heart–brainstem preparation over 25 years

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Autor(es): dc.contributorUniversity of Auckland-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversity of Bristol-
Autor(es): dc.contributorNational Institutes of Health-
Autor(es): dc.contributorUniversity of Melbourne-
Autor(es): dc.contributorUniversity of Calgary-
Autor(es): dc.contributorMount Royal University-
Autor(es): dc.contributorAix-Marseille Université-
Autor(es): dc.contributorGeisel School of Medicine at Dartmouth-
Autor(es): dc.creatorPaton, Julian F. R.-
Autor(es): dc.creatorMachado, Benedito H.-
Autor(es): dc.creatorMoraes, Davi J. A.-
Autor(es): dc.creatorZoccal, Daniel B.-
Autor(es): dc.creatorAbdala, Ana P.-
Autor(es): dc.creatorSmith, Jeffrey C.-
Autor(es): dc.creatorAntunes, Vagner R.-
Autor(es): dc.creatorMurphy, David-
Autor(es): dc.creatorDutschmann, Mathias-
Autor(es): dc.creatorDhingra, Rishi R.-
Autor(es): dc.creatorMcAllen, Robin-
Autor(es): dc.creatorPickering, Anthony E.-
Autor(es): dc.creatorWilson, Richard J. A.-
Autor(es): dc.creatorDay, Trevor A.-
Autor(es): dc.creatorBarioni, Nicole O.-
Autor(es): dc.creatorAllen, Andrew M.-
Autor(es): dc.creatorMenuet, Clément-
Autor(es): dc.creatorDonnelly, Joseph-
Autor(es): dc.creatorFelippe, Igor-
Autor(es): dc.creatorSt-John, Walter M.-
Data de aceite: dc.date.accessioned2025-08-21T21:57:05Z-
Data de disponibilização: dc.date.available2025-08-21T21:57:05Z-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2023-03-01-
Data de envio: dc.date.issued2022-05-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1113/JP281953-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/240863-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/240863-
Descrição: dc.descriptionTwenty-five years ago, a new physiological preparation called the working heart–brainstem preparation (WHBP) was introduced with the claim it would provide a new platform allowing studies not possible before in cardiovascular, neuroendocrine, autonomic and respiratory research. Herein, we review some of the progress made with the WHBP, some advantages and disadvantages along with potential future applications, and provide photographs and technical drawings of all the customised equipment used for the preparation. Using mice or rats, the WHBP is an in situ experimental model that is perfused via an extracorporeal circuit benefitting from unprecedented surgical access, mechanical stability of the brain for whole cell recording and an uncompromised use of pharmacological agents akin to in vitro approaches. The preparation has revealed novel mechanistic insights into, for example, the generation of distinct respiratory rhythms, the neurogenesis of sympathetic activity, coupling between respiration and the heart and circulation, hypothalamic and spinal control mechanisms, and peripheral and central chemoreceptor mechanisms. Insights have been gleaned into diseases such as hypertension, heart failure and sleep apnoea. Findings from the in situ preparation have been ratified in conscious in vivo animals and when tested have translated to humans. We conclude by discussing potential future applications of the WHBP including two-photon imaging of peripheral and central nervous systems and adoption of pharmacogenetic tools that will improve our understanding of physiological mechanisms and reveal novel mechanisms that may guide new treatment strategies for cardiorespiratory diseases.-
Descrição: dc.descriptionSIDS Calgary Society-
Descrição: dc.descriptionHealth Research Council of New Zealand-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionAgence Nationale de la Recherche-
Descrição: dc.descriptionAustralian Research Council-
Descrição: dc.descriptionBritish Heart Foundation-
Descrição: dc.descriptionNational Institutes of Health-
Descrição: dc.descriptionManaaki Manawa – The Centre for Heart Research Faculty of Medical & Health Science University of Auckland, Auckland-
Descrição: dc.descriptionDepartment of Physiology School of Medicine of Ribeirão Preto University of São Paulo, São Paulo-
Descrição: dc.descriptionDepartment of Physiology and Pathology School of Dentistry of Araraquara São Paulo State University, São Paulo-
Descrição: dc.descriptionSchool of Physiology Pharmacology and Neuroscience Faculty of Biomedical Sciences University of Bristol-
Descrição: dc.descriptionCellular and Systems Neurobiology Section National Institute of Neurological Disorders and Stroke National Institutes of Health-
Descrição: dc.descriptionDepartment of Physiology and Biophysics Institute of Biomedical Sciences University of São Paulo-
Descrição: dc.descriptionMolecular Neuroendocrinology Research Group Bristol Medical School: Translational Health Sciences University of Bristol-
Descrição: dc.descriptionFlorey institute of Neuroscience and Mental Health University of Melbourne-
Descrição: dc.descriptionDepartment of Physiology and Pharmacology Hotchkiss Brain Institute and Alberta Children's Hospital Research Institute Cumming School of Medicine University of Calgary-
Descrição: dc.descriptionDepartment of Biology Faculty of Science and Technology Mount Royal University-
Descrição: dc.descriptionDepartment of Anatomy & Physiology University of Melbourne-
Descrição: dc.descriptionInstitut de Neurobiologie de la Méditerranée INMED UMR1249 INSERM Aix-Marseille Université-
Descrição: dc.descriptionDepartment of Medicine Faculty of Medical and Health Sciences University of Auckland-
Descrição: dc.descriptionEmeritus Department of Physiology and Neurobiology Geisel School of Medicine at Dartmouth-
Descrição: dc.descriptionDepartment of Physiology and Pathology School of Dentistry of Araraquara São Paulo State University, São Paulo-
Descrição: dc.descriptionHealth Research Council of New Zealand: 19/687-
Descrição: dc.descriptionCNPq: 313719/2020-9-
Descrição: dc.descriptionCNPq: 437375/2018-8-
Descrição: dc.descriptionAgence Nationale de la Recherche: ANR-21-CE14-0009-01-
Descrição: dc.descriptionAustralian Research Council: DP170104582-
Descrição: dc.descriptionBritish Heart Foundation: Lectureship 1994-
Descrição: dc.descriptionNational Institutes of Health: U01 EB021960-
Formato: dc.format2049-2075-
Idioma: dc.languageen-
Relação: dc.relationJournal of Physiology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectcardiac ganglion-
Palavras-chave: dc.subjectcardiac vagus-
Palavras-chave: dc.subjectchemoreceptor-
Palavras-chave: dc.subjecteupnoea-
Palavras-chave: dc.subjectgasp-
Palavras-chave: dc.subjecthypertension-
Palavras-chave: dc.subjecthypoxia-
Palavras-chave: dc.subjectKölliker–Fuse-
Palavras-chave: dc.subjectpre-Bötzinger complex-
Palavras-chave: dc.subjectrespiratory rhythm generation-
Palavras-chave: dc.subjectsympathetic-
Palavras-chave: dc.subjectsympathetic-respiratory coupling-
Título: dc.titleAdvancing respiratory–cardiovascular physiology with the working heart–brainstem preparation over 25 years-
Tipo de arquivo: dc.typevídeo-
Aparece nas coleções:Repositório Institucional - Unesp

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